Analysis of Chemical Constituents in Oroxyli Semen by UPLC-Q-TOF-MS

Yin WU; Wan-jun BAI; Xin WEI

doi:10.13422/j.cnki.syfjx.20181916

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Analysis of Chemical Constituents in Oroxyli Semen by UPLC-Q-TOF-MS

Quality Evaluation | 更新时间：2021-02-09

- Analysis of Chemical Constituents in Oroxyli Semen by UPLC-Q-TOF-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 2, Pages: 196-200(2019)
- 作者机构：
  
  1.河北省人民医院，石家庄　 050071;
  2.中国中医科学院　中药研究所，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181916
  CLC： R284.1;R289;R22;R2-031
- Received：04 April 2018，
  
  Published Online：13 July 2018，
  
  Published：20 January 2019
- 稿件说明：
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Yin WU, Wan-jun BAI, Xin WEI. Analysis of Chemical Constituents in Oroxyli Semen by UPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 196-200.
DOI：

Yin WU, Wan-jun BAI, Xin WEI. Analysis of Chemical Constituents in Oroxyli Semen by UPLC-Q-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 196-200. DOI： 10.13422/j.cnki.syfjx.20181916.

摘要

目的：

中药木蝴蝶中主要含有黄酮类化学成分，具有抗炎、抗菌、抗氧化等药理作用。该研究采用超高效液相色谱串联四级杆飞行时间质谱(UPLC-Q-TOF-MS)技术对木蝴蝶中种类繁多的黄酮和黄酮苷类成分进行了快速全面的分析。

方法：

采用Kinetex-XB C

色谱柱(2.1 mm × 50 mm，1.7 μm)，以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱，流速设定为0.25 mL·min

－1

；进样量3 μL。质谱使用ESI离子源，负离子模式扫描采集数据。通过Peakview 2.2和Masterview 1.0软件进行目标及非目标筛查化合物，根据精确质量数和同位素峰度比及二级谱图比对完成目标化合物识别，通过裂解规律分析，结合已有文献报道，确定非目标化合物结构式。

结果：

根据精确相对分子质量和碎片离子分析，并结合相关文献检索及质谱裂解规律，共鉴定出木蝴蝶中36个黄酮类化学成分，其中包括10个黄酮苷元和26个黄酮苷类成分。

结论：

采用UPLC-Q-TOF-MS技术建立了快速分析木蝴蝶黄酮类化学成分的方法，该法快速、准确，为木蝴蝶的化学成分鉴定提供一种新的策略，并为木蝴蝶进一步质量控制和物质基础研究提供了技术支持。

Abstract

Objective:

Flavonoids

as the major constituents in Oroxyli Semen

had a variety of pharmacological effects against inflammation

infections

and oxidation. In this study

an ultra-high performance liquid chromatography (UPLC-Q-TOF-MS) method was established to analyze and identify the various flavonoids in Oroxyli Semen.

Method:

The separation was performed on an Kinetex-XB C

column(2.1 mm × 50 mm

1.7 μm)with gradient elution

which was carried out with 0.1%formic acid acetonitrile as mobile phase. The flow rate was 0.25 mL·min

-1

and the injection volume was 3 μL. Electrospray ionization source was in the negative ion mode. Target and non-target screenings were carried out by Peakview 2.2 and Masterview 1.0 software. Then the identification of target compounds were completed based on accurate mass

isotopic abundance ratio and MS/MS fragment

and the non-target compounds were determined according to literature reports and MS cleavage mechanism.

Result:

According to the high resolution MS spectra data

fragmentation ion information and retention time

36 peaks of Oroxyli Semen were identified

including 10 flavonoids and 26 flavonoid glycosides according to literature reports and MS cleavage mechanism.

Conclusion:

The study comprehensively analyzes the chemical composition of Oroxyli Semen

which is significant to study of the chemical constituents

quality control and material basis of Oroxyli Semen.

关键词

Keywords

references

文景兵，张庆文，殷志琦，等．木蝴蝶种子中黄酮类化学成分研究 [J]. 中国药学杂志， 2011 , 46 ( 3 ): 170 - 173 .

殷文光，李曼玲，康琛．木蝴蝶的研究进展 [J]. 中国中药杂志， 2007 , 32 ( 19 ): 1965 - 1970 .

王锐，何嵋，袁晓春，等．木蝴蝶总黄酮的抗氧化活性 [J]. 中国实验方剂学杂志， 2012 , 18 ( 23 ): 102 - 105 .

YAN R Y , CAO Y Y. Antioxidant flavonoids from the seed of Oroxylum indicum [J]. Fitoterapia , 2011 , 82 ( 6 ): 841 - 848 .

ZHANG B W , SANG Y B , SUN W L , et al . Combination of flavonoids from Oroxylum indicum seed extracts and acarbose improves the inhibition of postprandial blood glucose: in vivo and in vitro study [J]. Biomed Pharmacother , 2017 , 91 : 890 - 898 .

李楠楠，孟宪生，包永睿，等．木蝴蝶挥发性成分对H-(22)荷瘤小鼠实体瘤抑制作用及其机制初步研究 [J]. 中国药理学通报， 2017 , 33 ( 11 ): 1600 - 1605 .

李慧，魏梅，陈向东． HPLC法同时测定木蝴蝶配方颗粒中木蝴蝶苷B和黄芩苷的含量 [J]. 广东药学院学报， 2014 , 30 ( 6 ): 713 - 716 .

曹阳阳，晏仁义，杨滨，等． HPLC测定广西木蝴蝶中木蝴蝶苷B和黄芩苷的含量 [J]. 中国实验方剂学杂志， 2012 , 18 ( 4 ): 82 - 84 .

张昌壮．木蝴蝶化学成分的研究 [D]．长春：吉林大学， 2011 .

陈亮亮，宋晓凯，侯文彬，等．木蝴蝶化学成分的研究 [J]. 中草药， 2007 , 38 ( 2 ): 186 - 187 .

杨维，王树瑶，吴思玚，等．基于PREC-IDA-EPI技术的黄芩中黄酮成分研究 [J]. 中国中药杂志， 2018 , 43 ( 2 ): 345 - 352 .

Nair A G , Romachandran , Joshi B S. Oroxindin-a new flavones glueuronide from Oroxylum indicum [J]. Proc Indian Acad Sci,Sect A , 1979 , 88 ( 5 ): 323 - 327 .

文景兵，张庆文，殷志琦，等 . 木蝴蝶种子中黄酮类化学成分研究 [J]. 中国药学杂志， 2011 , 46 ( 3 ): 170 - 173 .

冯阳，陈玉梅，辛华．金樱子黄酮类成分的UPLC-Q-TOF-MS分析 [J]. 中国实验方剂学杂志， 2017 , 23 ( 12 ): 71 - 76 .

翟翔宇，萧伟，杨彪，等．木蝴蝶种子的化学成分研究 [J]. 中国中药杂志， 2015 , 40 ( 15 ): 3013 - 3016 .

李云贵．木蝴蝶总黄酮提取纯化及药理活性的研究 [D]．衡阳：南华大学， 2014 .

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