Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia

SU Shan; WANG Rong; ZHAO Min; WU Chen; GAO Hui-qin

doi:10.13422/j.cnki.syfjx.20181933

您当前的位置：

首页 >

文章列表页 >

Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia

更新时间：2024-01-04

- Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 19, Pages: 159-165(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181933
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
SU Shan, WANG Rong, ZHAO Min, et al. Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 159-165.
DOI：

SU Shan, WANG Rong, ZHAO Min, et al. Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 159-165. DOI： 10.13422/j.cnki.syfjx.20181933.

摘要

目的：观察秦艽不同配伍药对对风寒湿痹型类风湿性关节炎（rheumatoid arthritis，RA）模型大鼠血清类风湿因子（rheumatoid factor，RF），C-反应蛋白（C-reactive protein，CRP）及踝关节核转录因子-κB（nuclear factor kappa B，NF-κB）的影响。方法：选取80只SD大鼠并随机分为正常组，Ⅱ型胶原组，风寒湿病证模型组，雷公藤多苷组，单味秦艽组，秦威组（秦艽配伍威灵仙），秦桑组（秦艽配伍桑寄生），秦防组（秦艽配伍防己），每组10只。注射Ⅱ型胶原乳剂并施加风寒湿刺激制备风寒湿RA大鼠模型。模型建立完成后正常组，Ⅱ型胶原组，风寒湿病证模型组给予生理盐水灌胃，各给药组给予相应药液。实验过程中，每3 d测量一次大鼠左后足跖厚度，计算足跖肿胀度；实验第38天对各组大鼠进行关节炎指数（arthritis index，AI）评分；酶联免疫吸附测定法（ELISA）检测大鼠血清RF，CRP含量；蛋白免疫印迹技术检测踝关节NF-κB的表达；实时荧光定量聚合酶链式反应（Real-time PCR）技术检测踝关节NF-κB mRNA表达。结果：与正常组比较，Ⅱ型胶原组及风寒湿病证模型组足跖肿胀度，AI评分，血清RF，CRP水平，踝关节NF-κB蛋白表达水平及NF-κB mRNA水平显著升高（P<0.01）；与风寒湿病证模型组比较，各中药组足跖肿胀度，AI评分，血清RF，CRP水平，踝关节NF-κB蛋白表达水平及NF-κB mRNA水平均有所降低（P<0.05，P<0.01），其中秦威组降低最明显。结论：平温相配的秦威组对于风寒湿痹型类风湿关节炎的疗效优于平平相配的秦桑组、平寒相配的秦防组及单味秦艽组，实验结果与中医"疗寒以热药"的原则基本相符。

Abstract

Objective: To observe the effects of differences combined administrations of Gentianae Macrophyllae Radix (GMR) on serum rheumatoid factor (RF)

C-reactive protein (CRP) and ankle nuclear factor kappa B (NF-κB) in rheumatoid arthritis (RA) model rats with wind-cold-dampness arthralgia. Method: Eighty healthy SD rats were randomly divided into blank control group

collage Ⅱ model group

wind-cold-dampness syndrome model group

positive control group

single-taste GMR group

GMR-Clematidis Radix et Rhizoma(CRR) group (GC group)

GMR-Taxilli Herba group (GT group)

GMR-Stephaniae Tetrandrae Radix group (GS group)

with 10 rats in each group. Wind-cold-dampness Arthralgia model rats was induced in each group except for the blank group; collage Ⅱ model group was injected with collage Ⅱ and exposed to a wind-cold-dampness environment to prepare the wind-cold-dampness RA model of rats. After the establishment of the model

blank control group

collage Ⅱ model group and wind-cold-dampness syndrome model group were given normal saline

and the corresponding liquid medicines were given to each administration group. In the experiment

the thickness of the left posterior metatarsal of rats was measured every 3 days

and the swelling degree of the metatarsal was calculated. The arthritis index (AI) was evaluated on the 38th day of the experiment. The serum RF and CRP contents of rats were detected by enzyme linked immunosorbent assay (ELISA). The expression of NF-κB in ankle joint was detected by Western blot. The NF-κB mRNA expression in ankle joint was detected by Real-time fluorescence quantitative PCR. Result: Compared with the blank group

the swelling degree

AI score of foot

serum RF and CRP levels

of NF-κB protein and mRNA expressions in the ankle joint of collage Ⅱ model group and wind-cold-dampness syndrome model group were significantly increased (P<0.01). Compared with the wind-cold-dampness syndrome model group

the swelling degree

AI score of foot

serum RF and CRP levels

NF-κB protein and mRNA expressions in the ankle joint of the traditional Chinese medicine group were decreased(P<0.05

P<0.01)

especially in GC group. Conclusion: The therapeutic effect of mild and warm traditional Chinese medicine (TCM) is better than that of the combined administration of mild and cold TCM or mild TCM drugs for rheumatoid arthritis with wind-cold-dampness arthralgia. The experimental results basically conform to the principle of ‘treating cold diseases with hot medicine’ in TCM.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Component Compatibility of Gentianae Macrophyllae Radix and Clematidis Radix et Rhizoma on Serum Inflammatory Factors and Expression of NF-κB and VEGF in Ankle Joint of Rats with Rheumatoid Arthritis

Effect of Different Combinations of Gentianae Macrophyllae Radix on MMP-3 and TIMP-1 in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia

Editorial Explanation of Guidelines for Establishing Animal Models of Rheumatoid Arthritis with Cold-dampness Obstruction Syndrome and Dampness-heat Obstruction Syndrome

Guidelines for Establishing Animal Models of Rheumatoid Arthritis with Cold-dampness Obstruction Syndrome and Dampness-heat Obstruction Syndrome

Related Author

GUO Congjia

WU Guotai

GAO Huiqin

ZHENG Lixia

LI Sa

WANG Liming

HAO Qianying

Shan SU

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Gansu University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Southern Medical University Hospital of Integrated Chinese and Western Medicine

Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰