Objective: To study on the effect of Xiaoyaowan on the endogenous metabolites in the urine of rats with nonalcoholic fatty liver disease(NAFLD) and liver depression and spleen deficiency syndrome

and to explore the therapeutic mechanism of this compound. Method: SD rats were randomly divided into 3 groups

namely blank group

model group and Xiaoyaowan group(3.24 g·kg-1·d-1).The high-fat and high-fat diet combined with irregular diet

chronic restraint stress stimulation

and tail clipping method were employed to replicate the NAFLD model for 10 weeks.Xiaoyaowan was given for 4 weeks.The levels of serum alanine aminotransferase

total cholesterol and triglycerides in the hepatic tissue

5-hydroxytryptamine and norepinephrine in brain tissue of model rats were detected by enzyme-linked immunosorbent assay.The morphology of liver tissue was observed by hematoxylin-eosin(HE) staining.The nucleus magnetic data of model rat urine was collected by metabolomics and combined with multivariate statistical analysis to explore the therapeutic mechanism of Xiaoyaowan. Result: Xiaoyaowan could make acetic acid

creatinine

glycine and other nine markers in the urine of rats with NAFLD and syndrome of liver depression and spleen deficiency have varying degrees of callback.It mainly involved the regulation of metabolic pathways

such as energy metabolism

glucose metabolism

insulin resistance

etc. Conclusion: Xiaoyaowan may play a role in the treatment of NAFLD by regulating lipid metabolism

glucose and amino acid metabolism disorders.