Evaluation of Hepatoprotective Effect of Combined Administration of  and  Fructus by Uniform Design

NI Jun; LIU Rui; WANG Xin-zhi; BIAN Hui-min; CHENG Jian-ming; Wang Qing; WU Hao

doi:10.13422/j.cnki.syfjx.20182009

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Evaluation of Hepatoprotective Effect of Combined Administration of and Fructus by Uniform Design

更新时间：2024-09-23

- Evaluation of Hepatoprotective Effect of Combined Administration of and Fructus by Uniform Design
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 22, Pages: 40-44(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182009
  CLC： R289
- Published：2018
- 稿件说明：
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NI Jun, LIU Rui, WANG Xin-zhi, et al. Evaluation of Hepatoprotective Effect of Combined Administration of and Fructus by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 40-44.
DOI：

NI Jun, LIU Rui, WANG Xin-zhi, et al. Evaluation of Hepatoprotective Effect of Combined Administration of and Fructus by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 40-44. DOI： 10.13422/j.cnki.syfjx.20182009.

摘要

目的：筛选并优化五味子与杂色蛤配伍比例，并考察其对小鼠肝损伤的保护作用。方法：采用均匀设计，以小鼠血清中丙氨酸氨基转移酶（ALT）和天门冬氨酸氨基转移酶（AST）活力为指标，考察五味子与杂色蛤不同配比对四氯化碳（CCl4）肝损伤小鼠的保护作用，通过SAS 9.3软件拟合曲线，得到最佳配比，选取最佳配比下的不同剂量，分别通过小鼠CCl4急性肝损伤模型和酒精性急性肝损伤模型进行验证试验。结果：五味子与杂色蛤配伍组均能显著降低血清中AST和ALT活力，经回归分析可知AST和ALT活力随五味子剂量的增加先降低后升高，随杂色蛤剂量增加而降低，但两者未见交互作用。确定五味子与杂色蛤最佳配伍剂量分别为0.30，0.57 g·kg-1，即配比为1：1.9；验证试验结果表明该比例下五味子与杂色蛤的配伍组保肝效果显著且优于二者单用组。结论：五味与杂色蛤配伍后具有保肝作用，为杂色蛤资源的开发与利用提供了实验依据。

Abstract

Objective:To screen and optimize the proper proportion of Ruditapes philippinarum(RP) and Schisandra chinensis fructus(SCF)

and investigate its protective effect on liver injury in mice. Method:Taking activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in mice serum as indexes

uniform design was applied to investigate protective effect of RP and SCF with different proportion on liver injury mice induced by CCl4.The optimum proportion of RP to SCF was selected according to curve fitted by SAS 9.3 software.Validation of optimized proportion was implemented on the model of both CCl4 and alcohol induced mice liver injury at various dose levels. Result:Compatibility group of RP and SCF could significantly decrease the activities of AST and ALT in mice serum.The results of regression analysis showed that the activities of ALT and AST decreased with increasing of dose level of RP

and first decreased and then increased with increasing of dose level of SCF;however

there was no interactive effect between dose levels of RP and SCF.The optimum compatibility doses of RP and SCF were 0.57

0.30 g·kg-1

namely

the ratio of SCF to RP was 1:1.9.The validation experiment also showed that the compatibility group was better than the single group at the optimum dose. Conclusion:The compatibility of RP and SCF can protect liver.The study provides experimental basis for the development and utilization of RP resources.

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