Determination of Stachydrine Hydrochloride Content in Leonurus Granule by HPLC-ELSD

LI Wei; ZHANG Jian-jun; WANG Xiao-hua; WANG Yu-jie; TANG Ru-ying; WANG Lin-yuan

doi:10.13422/j.cnki.syfjx.20182018

您当前的位置：

首页 >

文章列表页 >

Determination of Stachydrine Hydrochloride Content in Leonurus Granule by HPLC-ELSD

更新时间：2024-09-23

- Determination of Stachydrine Hydrochloride Content in Leonurus Granule by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 23, Pages: 100-103(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182018
  CLC： R286.0
- Published：2018
- 稿件说明：
移动端阅览
LI Wei, ZHANG Jian-jun, WANG Xiao-hua, et al. Determination of Stachydrine Hydrochloride Content in Leonurus Granule by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 100-103.
DOI：

LI Wei, ZHANG Jian-jun, WANG Xiao-hua, et al. Determination of Stachydrine Hydrochloride Content in Leonurus Granule by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 100-103. DOI： 10.13422/j.cnki.syfjx.20182018.

摘要

目的：建立HPLC-ELSD测定益母草颗粒中盐酸水苏碱含量的方法。方法：供试品采用732钠型阳离子交换树脂处理；Venusil HILIC丙基酰胺键合硅胶色谱柱（4.6 mm×250 mm，5 μm）；柱温室温；流动相乙腈-0.2%冰乙酸溶液（80∶ 20）；流速1 mL·min-1；进样量10 μL。蒸发光散射检测器参数：漂移管温度79℃；载气体积流量2 L·min-1。结果：盐酸水苏碱在0.498~4.980 μg呈良好的线性关系（r=0.999 5，n=6）；无糖型益母草颗粒平均加样回收率为99.7%，RSD 2.0%（n=6）；有糖型益母草颗粒平均加样回收率为99.3%，RSD 1.9%（n=6）。结论：采用732钠型阳离子交换树脂精制和Venusil HILIC丙基酰胺键合硅胶柱、蒸发光散射检测器的检测方法测定，操作简便，结果准确，可作为益母草颗粒中盐酸水苏碱的含量测定方法，并可为益母草的其他单位制剂中盐酸水苏碱的含量测定提供参考及借鉴。

Abstract

Objective:To establish the methods of content determination of stachydrine hydrochloride in Leonurus granule by high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD). Method:The samples were treated with 732 sodium cation exchange resin. The determination was performed on Venusil HILIC column(4.6 mm×250 mm

5 μm);column temperature:room temperature;with acetonitrile and 0.2% acetic solution as mobile phase (80:20) at the flow rate 1 mL·min-1;sample amount 10 μL;drift tube temperature 79℃;air volume flow 2 L·min-1. Result:The stachydrine hydrochlorideshowed a good linear relationship within the range of 0.498-4.980 μg(r=0.999 5

n=6);and the average recovery was 99.7% and 99.3% respectively for sugar free Leonurus granule and sugar containing Leonurus granule. Conclusion:The method is specific

accurate and simple

which can be applied as a new way for content determination of Leonurus granule

and provide reference for content determination of stachydrine hydrochloride in other unilateral preparations of Leonuri Herb.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Euphol in Slices of and Kongxian Pills by HPLC-ELSD

Content Comparison of Myo-Inositol from of Different Varieties

Determination of Euphol in Slices of Euphorbia kansui and Kongxian Pills by HPLC-ELSD

Comparison of Quality Change During Processing Process of Achyranthis Bidentatae Radix from Different Origins Based on Color-component Correlation Analysis

Effect of Microemulsion on Content of Index Components in Different Phases of Zexietang Extract

Related Author

XIU Yan-feng

SHI Bei

ZHU Cheng

ZHANG Xu-qian

XIAO Feng-xia

DENG Shao-dong

DENG Chao-ming

DENG Tao

Related Institution

College of Traditional Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine

Key Laboratory of Processing Traditional Chinese Material Media Nanjing University of Traditional Chinese Medicine

Third Affiliated Hospital of Henan University of Chinese Medicine

Luohe Medical College

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰