Correlation Analysis Between HPLC Fingerprint and Free Radical-scavenging Activity of  Leaves

WANG Le; HE Ting; CHANG Yan-li; FAN Shu-sheng; WANG Xiu-huan; WANG Yan; LI Xiao; WANG Xiao-ping; XU Xiao; SUN Zhi-rong; SHE Gai-mei

doi:10.13422/j.cnki.syfjx.20182024

您当前的位置：

首页 >

文章列表页 >

Correlation Analysis Between HPLC Fingerprint and Free Radical-scavenging Activity of Leaves

更新时间：2024-09-23

- Correlation Analysis Between HPLC Fingerprint and Free Radical-scavenging Activity of Leaves
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 23, Pages: 93-99(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182024
  CLC： O657.72;R284.1
- Published：2018
- 稿件说明：
移动端阅览
WANG Le, HE Ting, CHANG Yan-li, et al. Correlation Analysis Between HPLC Fingerprint and Free Radical-scavenging Activity of Leaves[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 93-99.
DOI：

WANG Le, HE Ting, CHANG Yan-li, et al. Correlation Analysis Between HPLC Fingerprint and Free Radical-scavenging Activity of Leaves[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 93-99. DOI： 10.13422/j.cnki.syfjx.20182024.

摘要

目的：研究女儿茶Rhamnus heterophylla HPLC指纹图谱与其清除自由基活性的关系。方法：采用高效液相色谱法建立8批不同产地、采收期女儿茶指纹图谱，在传统相似度评价基础上，采用Origin Pro 2017软件进行层次聚类分析（hierarchical cluster analysis，HCA）和主成分分析（principal component analysis，PCA）对其指纹图谱中的共有峰进行评价；1，1-二苯基-2-三硝基苯肼（DPPH）法研究其清除自由基活性；偏最小二乘回归分析（partial least squares regression，PLSR）和灰色关联度分析（grey relational analysis，GRA）研究谱效关系。结果：建立了8批女儿茶HPLC指纹图谱，确定了11个共有峰，相似度在0.948～0.976。采用对照品比对方法指认了其中6个峰：x1为原儿茶酸，x2为木犀草苷，x5为槲皮素，x8为山柰酚，x10为大黄素8-O-α-L-鼠李糖苷，x11为大黄素。样本可聚为4类。8批女儿茶均有不同程度清除自由基活性。灰色关联度分析结果显示共有峰与清除自由基活性的关联度大小为x11 > x10 > x8 > x1 > x9 > x4 > x3 > x2 > x6 > x5 > x7。偏最小二乘回归分析建立的回归方程为Y=99.769 17-0.357 49x1-0.001 36x2-0.002 65x3+0.059 63x4+0.011 81x6+0.010 63x7-0.006 99x8-0.009 14x9+0.054 27x10+0.022 75x11。结论：女儿茶清除自由基活性是多组分联合效应的结果。

Abstract

Objective:To explore the correlation between high performance liquid chromatography (HPLC)fingerprint and free radical-scavenging activity of Rhamnus heterophylla leaves. Method:Fingerprint of eight batches of R. heterophylla leaves from different regions and harvest times was established by HPLC. Origin Pro 2017 software was used for hierarchical cluster analysis(HCA) and principal component analysis (PCA) to evaluate the common peaks in the fingerprint on the basis of traditional similarity evaluation method. The free radical-scavenging activity was determined by 1

1-diphenyl-2-picrylhydrazyl radical 2

2-diphenyl-1-(2

6-trinitrophenyl)hydrazyl (DPPH) assay. Partial least squares regression (PLSR) and grey relational analysis (GRA) were used to study the relationship between HPLC fingerprint and free radical-scavenging activity. Result:The HPLC fingerprint of eight batches of R. heterophylla leaves was established

and 11 common peaks were determined

with the similarity of 0.948-0.976.Six peaks among common peaks were identified by comparing to reference substances:x1

x10

x11 referred to protocatechic acid

luteoloside

quercetin

kaempferol

emodin-8-O-α-L-rhamnoside

and emodin

respectively. The samples were clustered into four categories. All of the samples had different degrees of scavenging free radical activity. The result of GRA showed that the order of correlation was x11 > x10 > x8 > x1 > x9 > x4 > x3 > x2 > x6 > x5 > x7.The regression equation established by PLSR analysis was Y=99.769 17-0.357 49x1-0.001 36x2-0.002 65x3+0.059 63x4+0.011 81x6+0.010 63x7-0.006 99x8-0.009 14x9+0.054 27x10+0.022 75x11. Conclusion:The free radical-scavenging activity of R. heterophylla leaves was the result of the combined effects of multiple components.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Spectrum-effect Relationship of Bupleuri Radix Processed with Trionyx sinensis Blood for Yin Deficiency Based on Saponins

Exploration of Spectrum-effect Relationship of Zhuriheng Dropping Pills Against Macrophage Foaming Based on UPLC-Q-Exactive Orbitrap MS

Material Basis and Mechanism of Epimedii Folium Against Osteoporosis Based on Spectrum-Effect Relationship and Network Pharmacology

Analysis on Spectrum-effect Relationship of Anti-inflammatory Effect of Zhideke Granules Based on Chemical Pattern Recognition

Investigation of Spectrum-effect Relationship of Brain Protection of Shaoyao Gancaotang on Epileptic Mice Based on Grey Correlation Analysis

Related Author

HOU Mengyu

ZHAO Xia

GUO Zhiyu

LIU Ting

MA Yuexing

YE Yaohui

ZHAI Qiong

LIANG Fangyuan

Related Institution

Nanchang Medical College

Jiangxi University of Chinese Medicine

School of Basic Medicine，School of Pharmacy，Inner Mongolia Medical University

Affiliated Hospital of Nanjing University of Chinese Medicine

Multi-component of Traditional Chinese Medicine （TCM） and Microecology Research Center， Jiangsu Province Academy of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰