Objective:To explore the effect and mechanism of olivine (OL) on glucose metabolism of liver in diabetic mice. Method:Totally 40 db/db mice of spontaneous diabetes were randomly divided into model group

metformin group (0.13 g·kg-1)

and high and low-dose olivine group (0.05

0.025 g·kg-1)

with 10 mice in each group

and 10 C57BL/6J mice were set as normal group. After 6 weeks of treatment

fasting blood glucose(FBG)

Fins

HOMA insulin resistance index(HOMA-IR)

oral glucose tolerance test (OGTT)

body weight

serum creatinine (SCr)

blood urea nitrogen(BUN)

aspartate aminotransferase(AST) and liver glycogen content were measured. Real-time PCR was used to detect the mRNA expressions of glucose-6-phosphatase (G-6-P) and phosphoenolpyruvate carboxykinase (p-EPCK). Western blot was used to detect the protein expressions of forehead box-containing protein of the O subfamily 1 (FoxO1) and protein kinase B (Akt). Result:Compared with model group

body weight

FBG

Fins and HOMA-IR in OL group decreased significantly (P<0.01); liver glycogen content increased significantly in OL group (P<0.05

P<0.01); blood glucose significantly decreased at 120 min of OGTT test (P<0.01); the mRNA expressions of p-EPCK

G-6-P in mouse liver decreased significantly (P<0.01)

and the phosphorylation levels of Akt

FoxO1 increased significantly (P<0.05

P<0.01). Conclusion:OL can inhibit gluconeogenesis

decrease FBG

and alliviate insulin resistance

which may be related to the increase of phosphorylation of Akt-FoxO1 and the decrease of transcription of p-EPCK and G-6-P.