Effect of Ginsenoside Rg on Invasion and Migration of Gastric Cancer BGC-823 Cells via miR-125b/STARD13/NEU1 Signaling Pathway

LI Wei; LIU Wei; ZOU Jun-jun

doi:10.13422/j.cnki.syfjx.20182026

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Effect of Ginsenoside Rg on Invasion and Migration of Gastric Cancer BGC-823 Cells via miR-125b/STARD13/NEU1 Signaling Pathway

更新时间：2024-09-23

- Effect of Ginsenoside Rg on Invasion and Migration of Gastric Cancer BGC-823 Cells via miR-125b/STARD13/NEU1 Signaling Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 22, Pages: 138-142(2018)
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- DOI：10.13422/j.cnki.syfjx.20182026
  CLC： R285
- Published：2018
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LI Wei, LIU Wei, ZOU Jun-jun. Effect of Ginsenoside Rg on Invasion and Migration of Gastric Cancer BGC-823 Cells via miR-125b/STARD13/NEU1 Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 138-142.
DOI：

LI Wei, LIU Wei, ZOU Jun-jun. Effect of Ginsenoside Rg on Invasion and Migration of Gastric Cancer BGC-823 Cells via miR-125b/STARD13/NEU1 Signaling Pathway[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 138-142. DOI： 10.13422/j.cnki.syfjx.20182026.

摘要

目的：研究人参皂苷Rg5对胃癌BGC-823细胞侵袭迁移的影响，并通过微小RNA-125b（microRNA-125b，miR-125b）/类固醇敏感性调节蛋白相关脂类转运体结构域13（StAR-related lipid transfer domain protein 13，STARD13）/唾液酸酶1（neuraminidase 1，NEU1）信号通路探讨人参皂苷Rg5抑制胃癌侵袭迁移的作用机制。方法：人参皂苷Rg5（12.5，25，50 μmol·L-1）处理BGC-823细胞后，采用transwell小室实验检测人参皂苷Rg5对BGC-823细胞侵袭、迁移能力的影响，实时荧光定量PCR（Real-time PCR）检测miR-125b，STARD13，NEU1 mRNA的表达，蛋白免疫印迹法（Western blot）检测STARD13，NEU1蛋白的表达。结果：人参皂苷Rg5（25，50 μmol·L-1）可有效抑制BGC-823细胞的侵袭、迁移。miR-125b，STARD13，NEU1 mRNA的表达，与空白组比较，人参皂苷Rg512.5 μmol·L-1组的miR-125b相对表达水平降低，STARD13，NEU1 mRNA相对表达水平升高，但无统计学差异；人参皂苷Rg5（25，50 μmol·L-1）组miR-125b相对表达水平降低，STARD13，NEU1 mRNA相对表达水平增高（P<0.01）。STARD13，NEU1蛋白的表达，与空白组比较，人参皂苷Rg5（12.5，25 μmol·L-1）组的STARD13蛋白相对表达水平升高，人参皂苷Rg5（12.5 μmol·L-1）组的NEU1蛋白相对表达水平升高，但差异无统计学意义；人参皂苷Rg5（50 μmol·L-1）组的STARD13蛋白相对表达水平升高（P<0.05），人参皂苷Rg5（25，50 μmol·L-1）组NEU1蛋白相对表达水平升高（P<0.01）。结论：人参皂苷Rg5可能通过降低miR-125b的表达，上调miR-125b靶基因STARD13，NEU1的表达，抑制胃癌BGC-823细胞的侵袭、迁移。

Abstract

Objective:To study the effect of ginsenoside Rg5(12.5

50 μmol·L-1) on the invasion and migration and the expressions of microRNA-125b(miR-125b)

StAR-related lipid transfer domain protein 13(STARD13) and neuraminidase 1 (NEU1)in gastric cancer BGC-823 cell

in order to investigate the possible mechanism of ginsenoside Rg5 in inhibiting the invasion and migration of gastric cancer. Method:Transwell was used to detect the invasion and migration of gastric cancer BGC-823 cell

Real-time PCR was used to detect the mRNA expressions of miR-125b

STARD13 and NEU1

and Western blot was used to detect the protein expressions of miR-125b

STARD13 and NEU1. Result:Middle and high-dose ginsenoside Rg5 could effectively inhibit the invasion and migration of BGC-823 cells. Compared with the blank group

the relative expression of miR-125b in low-dose group was decreased

the relative expressions of STARD13 and NEU1 mRNA in the low-dose group were increased; the relative expression of miR-125b in middle and high-dose groups was decreased

the relative expressions of STARD13 and NEU1 mRNA in middle and high-dose groups were increased(P<0.01). Compared with the blank group

the relative expression of STARD13 protein in low and middle-dose groups were increased

and NEU1 protein in low-dose group was increased; the relative expression of STARD13 protein in high-dose group was increased(P<0.05)

and NEU1 protein in middle and high-dose groups was increased(P<0.01). Conclusion:Ginsenoside Rg5 may inhibit the invasion and migration of gastric cancer BGC-823 cells by decreasing the expression of miR-125b and up-regulating the expressions of miR-125b target genes STARD13 and NEU1.

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