Objective:To observe the mechanism of Humifuse euphorbia on tumor angiogenesis in H22 liver cancer mice by regulating signal pathway of nuclear factor kappa-B/vascular endothelial growth factor (NF-κB/VEGF). Method:The models of SPF grade Kunming mice with H22 liver cancer were established through armpit injection. The animals with successful modeling were randomly divided into five groups:model control group

cyclophosphamide group (ip

50 mg·kg-1)

high-dose group (ig

4.50 g·kg-1)

middle-dose group (ig

2.25 g·kg-1) and low-dose group (ig

1.13 g·kg-1)

with 10 mice in each group. Their weights and tumor weights were measured after the 14-day drug administration. Index of tumor was calculated. Tumor micro-vessel density

which was marked as platelet endothelial cell adhesion molecule-31

was detected by immunohistochemitry. Tumor tissue NF-κB

VEGF

tumor necrosis factor-α(TNF-α)mRNA expressions were detected by polymerase chain reaction and protein expression by immunohistochemitry. Result:Compared with model group

H. euphorbia could significantly reduce tumor weigh (P<0.01). High-dose group showed an inhibition rate of 59.1% for H22 liver cancer in mice. CD31 expression of the high-dose group was significantly lower than that of the model group

while the expressions of NF-κB

TNF-α

VEGF mRNA and protein in tumor tissue were significantly decreased (P<0.01). Conclusion:H. euphorbia can inhibit the growth of H22 liver cancer mice

and its mechanism is probably related to the inhibition of the growth of tumor angiogenesis

which may activate NF-κB/VEGF signaling pathway.