Effect of Kangxianling Decoction on TGF-β<sub>1</sub>/Smad Signal Pathway in Renal Fibrosis Rats

Jing JI; Li-qun HE

doi:10.13422/j.cnki.syfjx.20182036

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Effect of Kangxianling Decoction on TGF-β₁/Smad Signal Pathway in Renal Fibrosis Rats

Topic on Treatment of Renal Fibrosis with Kangxianling Formulas | 更新时间：2021-02-09

- Effect of Kangxianling Decoction on TGF-β₁/Smad Signal Pathway in Renal Fibrosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 69-75(2019)
- 作者机构：
  
  1.上海中医药大学，上海　 200123;
  2.上海中医药大学　附属曙光医院，上海　 200021
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182036
  CLC： R2-0;R22;R285.5;R289
- Received：23 April 2018，
  
  Published Online：30 July 2018，
  
  Published：05 January 2019
- 稿件说明：
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Jing JI, Li-qun HE. Effect of Kangxianling Decoction on TGF-β₁/Smad Signal Pathway in Renal Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 69-75.
DOI：

Jing JI, Li-qun HE. Effect of Kangxianling Decoction on TGF-β₁/Smad Signal Pathway in Renal Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 69-75. DOI： 10.13422/j.cnki.syfjx.20182036.

摘要

目的：

研究抗纤灵方对5/6肾切除所致肾纤维化大鼠的治疗效果，并探讨其分子作用机制。

方法：

将50只SD大鼠随机分为正常组（

=10），假手术组（

=10），5/6肾切除肾纤维化造模组（

=30）。将造模成功的大鼠随机分为模型组、抗纤灵组、氯沙坦钾组。氯沙坦钾组、抗纤灵方组每日分别给予氯沙坦钾（33.3 g·kg

－1

）及抗纤灵方（21 g·kg

－1

）灌胃治疗，其余组都用等体积的生理盐水。持续8周灌胃后处死，检测各组大鼠血肌酐（SCr），尿素氮（BUN），24 h尿蛋白（24 h-Pro）；苏木素-伊红（HE）染色观察肾脏组织病理学改变；马松（Masson）染色观察肾脏纤维化程度；蛋白免疫印迹法（Western blot）及实时荧光定量聚合酶链式反应（Real-time PCR）分别检测肾脏转化生长因子-

（TGF-

），Smad2，Smad3，Smad7蛋白和mRNA在肾脏组织中的表达。

结果：

与正常组和假手术组比较，模型组大鼠的SCr

BUN，24 h-Pro上升，纤维化面积和肾脏损伤评分增加，TGF-

，Smad2，Smad3 mRNA与蛋白表达升高，Smad7 mRNA与蛋白表达显著下降（

0.01）。与模型组相比，氯沙坦钾组、抗纤灵方组可减少纤维化面积，降低肾脏损伤评分，SCr

BUN，24 h-Pro

TGF-

，Smad2，Smad3 mRNA与蛋白的表达水平，Smad7 mRNA与蛋白表达显著上调（

0.01），但低于正常组与假手术组的水平。

结论：

中药抗纤灵方可通过抑制TGF-

/Smad通路来延缓肾纤维化。

Abstract

Objective:

To study the therapeutic effect of Kangxianling Decoction on renal fibrosis induced by 5/6 nephrectomization in rats

and discuss its action mechanism.

Method:

Totally 50 SD rats were randomly divided into normal control group (

=10)

sham-operation group (

=10)

and 5/6 nephrectomized renal fibrosis model group (

=30). After successful modeling

rats were randomly divided into the model group

Kangxianling group

and Losartan Potassium group. Rats in the Losartan Potassium group were administered with Losartan Potassium by gastrogavage; rats in the Kangxianling group were administered with Kangxianling by gastrogavage. Equal volume of distilled water was administered to rats in the other groups. Rats were sacrificed after 8 weeks of consecutive medication

and then serum creatinine (SCr)

urea nitrogen (BUN)

24 hour urine protein (24 h-Pro) were measured in each group. Hematoxylin-eosin (HE) staining was used to observe the renal pathological changes and the score of Kidney damage was made. Masson staining was used to observe the degree of renal fibrosis. Western blot and real-time fluorescent quantitative polymerase chain reaction(Real-time PCR)were used to detect the protein and mRNA expression levels of transforming growth factor (TGF-

)

Smad2

Smad3

and Smad7 in kidneys.

Result:

As compared with the normal control and sham-operation group

the renal tissue fibrosis

score of kidney damage

SCr

BUN and 24 h-Pro levels were higher in model group (

0.01)

with increased protein and mRNA expression levels of TGF-

Smad2

and Smad3 (

0.01)

and significantly reduced protein and mRNA expression levels of Smad7 in the model group (

0.01). As compared with the model group

the renal tissue fibrosis

the score of kidney damage

SCr

BUN

24 h-Pro level were significantly decreased (

0.01)

protein and mRNA expression levels of TGF-

Smad2

Smad3 were reduced (

0.05)

while protein and mRNA expression levels of Smad7 were elevated in the Losartan Potassium group and Kangxianling group (

0.01)

but lower than those of normal control group and sham-operation group.

Conclusion:

Kangxianling decoction could alleviate renal fibrosis by inhibiting TGF-

/Smad signal pathway.

关键词

Keywords

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⁰

Effect of Kangxianling Decoction on TGF-β1/Smad Signal Pathway in Renal Fibrosis Rats

DOI：10.13422/j.cnki.syfjx.20182036

摘要

Abstract

关键词

Keywords

references

Effect of Kangxianling Decoction on TGF-β₁/Smad Signal Pathway in Renal Fibrosis Rats