Effect of Yangyin Shugan Granule on Expression of Bcl-2, Bax and Caspase-3 Protein in Rats with VCD-induced Diminished Ovarian Function

WANG Ji-ju; LIU Jian; HUANG Xu-chun; ZENG Yu-yan; WANG Xiao-yun

doi:10.13422/j.cnki.syfjx.20182040

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Effect of Yangyin Shugan Granule on Expression of Bcl-2, Bax and Caspase-3 Protein in Rats with VCD-induced Diminished Ovarian Function

更新时间：2024-01-04

- Effect of Yangyin Shugan Granule on Expression of Bcl-2, Bax and Caspase-3 Protein in Rats with VCD-induced Diminished Ovarian Function
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 20, Pages: 159-163(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20182040
  CLC： R285.5
- Published：2018
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WANG Ji-ju, LIU Jian, HUANG Xu-chun, et al. Effect of Yangyin Shugan Granule on Expression of Bcl-2, Bax and Caspase-3 Protein in Rats with VCD-induced Diminished Ovarian Function[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 159-163.
DOI：

WANG Ji-ju, LIU Jian, HUANG Xu-chun, et al. Effect of Yangyin Shugan Granule on Expression of Bcl-2, Bax and Caspase-3 Protein in Rats with VCD-induced Diminished Ovarian Function[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 159-163. DOI： 10.13422/j.cnki.syfjx.20182040.

摘要

目的：探讨养阴舒肝颗粒对卵巢功能下降模型大鼠卵巢功能及；B淋巴细胞瘤-2（Bcl-2），Bcl-2相关X蛋白（Bax），半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）蛋白的影响。方法：选取动情周期规则的雌性SD大鼠，取10只作为正常组，其余大鼠均采用160 mg·kg-1去氧乙烯基环己烯（VCD）腹腔注射诱导卵巢功能下降模型，取造模成功的56只大鼠按体质量随机分为模型组，养阴舒肝颗粒低（2.1 g·kg-1），中（4.2 g·kg-1），高剂量组（8.4 g·kg-1）和坤泰胶囊组，每组11只（模型组12只）。采用养阴舒肝颗粒和坤泰胶囊进行灌胃给药，干预治疗4周后进行卵巢称质量，计算各级卵泡的数目，酶联免疫吸附测定法（ELISA）检测血清性激素水平卵泡刺激素（FSH），雌二醇（E2），抗缪勒氏管激素（AMH），蛋白免疫印迹法（Western blot）检测卵巢组织Bcl-2，Bax，Caspase-3的表达量。结果：与正常组比较，模型组大鼠的卵巢质量和卵巢指数下降（P<0.01），闭锁卵泡数目增多（P<0.01），FSH水平升高（P<0.01），E2和AMH水平下降（P<0.05），卵巢组织Bcl-2蛋白的表达减少（P<0.01），Bax，Caspase-3蛋白的表达（P<0.01）；与模型组比较，养阴舒肝颗粒可以增加模型大鼠的双侧卵巢质量（P<0.05，P<0.01）；养阴舒肝颗粒和坤泰胶囊可以增加卵巢指数（P<0.05，P<0.01）；养阴舒肝颗粒可以减少闭锁卵泡的数目（P<0.05）；养阴舒肝颗粒和坤泰胶囊均可以降低FSH水平（P<0.05），升高E2和AMH水平（P<0.05），增加卵巢组织Bcl-2的表达（P<0.01），同时抑制Bax，Caspase-3的表达（P<0.01）。结论：养阴舒肝颗粒可以提高卵巢功能下降模型大鼠的卵巢功能，其机制可能与抗细胞凋亡有关。

Abstract

Objective: To investigate the effects of Yangyin Shugan granule(YYSG) on ovarian function in rats with diminished ovarian function

and explore its effect on B-cell lymphoma-2 (Bcl-2)

Bcl-2 associated X protein (Bax)

and cysteine-aspartic acid protease-3(Caspase-3) protein expression.Method: SD female rats with estrous cycle rules were selected

10 used as blank group

and the others were used to establish diminished ovarian function model by injecting 160 mg·kg-1 4-vinylcyclohexenediepoxide (VCD) into enterocoelia. 56 model rats with diminished ovarian function were randomly divided into 5 groups:model group

low-

middle-

high-dosage YYSG groups

and Kuntai capsule group

n=11 on each group (n=12 in model group). The YYSG groups (2.1

4.2

8.4 g·kg-1) and Kuntai capsule group had intragastric administration for 4 weeks after the model establishment. Then the ovarian weight was measured and follicle count was calculated. The levels of serum sex hormones[follicule-stimulating hormone (FSH)

estradiol (E2)

and anti-mullerian hormone (AMH)] were detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of Bcl-2

Bax

Caspase-3 in ovarian tissue were detected by Western blot.Result: As compared with the normal group

the ovary weight and ovary index were decreased (P<0.01)

Atresia follicle count was decreased (P<0.01)

FSH level was increased (P<0.01)

E2 and AMH levels were decreased (P<0.05)

Bcl-2 protein expressions were decreased (P<0.01)

and Bax and Caspase-3 protein expressions were increased(P<0.01) in model group. As compared with the model group

the ovary weights of YYSG groups were increased(P<0.05

P<0.01); YYSG granule and Kuntai capsule could increase the ovarian index(P<0.05

P<0.01); YYSG granule could reduce the numbers of atresia follicles(P<0.05); YYSG granule and Kuntai capsule could decrease FSH level but increase E2 and AMH levels(P<0.05)

increase the expression of Bcl-2 protein(P<0.01)

and decrease the expression levels of Bax

Caspase-3 protein(P<0.01).Conclusion: YYSG could improve the ovarian function of rats with diminished ovarian function and the mechanisms may be associated with anti-apoptosis.

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