Analysis of Chemical Constituents in Codonopsis Radix and Its  Antioxidant Activities

ZHANG Xin; LI Jian-kuan; ZHAO Yu-jing; GAO Jian-ping

doi:10.13422/j.cnki.syfjx.20182105

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Analysis of Chemical Constituents in Codonopsis Radix and Its Antioxidant Activities

更新时间：2024-09-23

- Analysis of Chemical Constituents in Codonopsis Radix and Its Antioxidant Activities
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 24, Pages: 53-59(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182105
  CLC： R284;R285
- Published：2018
- 稿件说明：
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ZHANG Xin, LI Jian-kuan, ZHAO Yu-jing, et al. Analysis of Chemical Constituents in Codonopsis Radix and Its Antioxidant Activities[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(24): 53-59.
DOI：

ZHANG Xin, LI Jian-kuan, ZHAO Yu-jing, et al. Analysis of Chemical Constituents in Codonopsis Radix and Its Antioxidant Activities[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(24): 53-59. DOI： 10.13422/j.cnki.syfjx.20182105.

摘要

目的：研究党参甲醇提取物中的化学成分，并对分离获得的化学成分进行体外抗氧化活性检测。方法：利用D101型大孔吸附树脂，MCI树脂，硅胶，十八烷基硅烷键合硅胶（ODS），羟丙基葡聚糖凝胶（Sephadex）LH-20等柱色谱及制备硅胶薄层色谱进行化合物分离，采用核磁共振（NMR）和质谱（MS）等方法进行化合物结构鉴定；采用1

1-二苯基-2-三硝基苯肼（DPPH），2

2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）和超氧阴离子自由基清除能力检测各化合物的抗氧化活性，以维生素C为阳性对照。结果：从党参的甲醇提取物中分离得到7个化合物，分别鉴定为7R/7S

8S-guaiacylglycerol-8-O-4'-（coniferyl alcohol）ether（1/2），党参炔苷（3），苍术内酯Ⅲ（4），紫丁香苷（5），党参苷Ⅰ（6），党参吡咯烷鎓B（7）。化合物1和2具有一定的DPPH和ABTS+·自由基清除能力，而化合物1~7对O2-·自由基的清除能力较弱。结论：化合物1和2为木脂素类中的新木脂素结构类型，为首次从党参属中分离得到，并具有一定的体外抗氧化活性。

Abstract

Objective: To study on the chemical constituents in Codonopsis Radix

and to investigate their in vitro antioxidant activities of isolated compounds. Method: The chemical constituents were isolated and purified by D101 macroporous resin

MCI resin

silica gel

octadecyl silane bonded silica(ODS)

Sephadex LH-20 column chromatography and preparative silica gel thin layer chromatography.Nuclear magnetic resonance(NMR) and mass spectrometry(MS) were used to elucidate the structures of obtained compounds.The antioxidant effects of isolated compounds were evaluated by radical scavenging ability of 1

1-diphenyl-2-picrylhydrazyl(DPPH)

2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS) and superoxide anion with vitamin C as positive control. Result: Seven compounds were obtained and identified as 7R/7S

8S-guaiacylglycerol-8-O-4'-(coniferyl alcohol) ether(1/2)

lobetyolin(3)

atractylenolide Ⅲ(4)

syringin(5)

tangshenoside Ⅰ(6)

codonopyrrolidium B(7).Among them

compound 1 and 2 showed potential capacity for DPPH and ABTS+·radical scavenging

compound 1-7 showed relatively low capacity for O2-·radical scavenging. Conclusion: Compound 1 and 2

identified as neolignans

are isolated from the genus Codonopsis for the first time and exhibit certain in vitro antioxidant activities.

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