Chinese medicinal materials are varied and multi-source

large difference in quality makes it difficult to ensure the safety and efficacy of clinical medications.Traditional identification methods have certain limitations

in some cases difficult to accurately identify. In order to ensure the safe and effective of traditional Chinese medicine (TCM)

TCM identification has become one of the most important research topics

which is a key technical requirement urgently needed to be solved in the current Chinese medicine business. DNA gene identification is a rapid and accurate identification of Chinese medicinal materials with the genetic level

includes DNA molecule genetic markers

DNA hybridization and DNA barcoding molecular identification method particularly the most widely used one is DNA barcoding molecular identification method.DNA barcode is a technique for identifying species by using standard DNA regions. The 2015 edition of the Chinese Pharmacopoeia has record the DNA barcode technical guidelines

which has established identify genes with COI gene-dominated and ITS2 gene-supplemented animal medicine DNA barcoding system

identify genes with ITS2/ITS gene-dominated and chloroplast psbA-trnH gene-supplemented plant medicine DNA barcoding system. The application of DNA Barcoding provides a more efficient and rapid method for the identification of TCM

and effectively applied in the identification of multi-primary Chinese herbal medicines. This article briefly outlines the principles

purpose and significance

selection of standard gene fragments of DNA barcodes

focuses on the application in identification of TCM

as well as the limitations. At last

the application forecast was prospected

which was expected to provide reference for Chinese medicine identification.