Mechanism of Salidroside in Improving Glucose and Lipid Metabolism in Liver of Diabetic Rats

Dan HOU; Guang-yuan XU; Zhuo ZHANG; Tong-hua LIU

doi:10.13422/j.cnki.syfjx.20182124

您当前的位置：

首页 >

文章列表页 >

Mechanism of Salidroside in Improving Glucose and Lipid Metabolism in Liver of Diabetic Rats

Pharmacology | 更新时间：2021-02-09

- Mechanism of Salidroside in Improving Glucose and Lipid Metabolism in Liver of Diabetic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 130-134(2019)
- 作者机构：
  
  1.北京中医药大学　中医养生学北京市重点实验室，北京　 100029
  2.首都医科大学　附属复兴医院，北京　 100045
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182124
  CLC： R22;R242;R2-031;R285.5
- Received：09 May 2018，
  
  Published Online：24 August 2018，
  
  Published：05 January 2019
- 稿件说明：
移动端阅览
Dan HOU, Guang-yuan XU, Zhuo ZHANG, et al. Mechanism of Salidroside in Improving Glucose and Lipid Metabolism in Liver of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 130-134.
DOI：

Dan HOU, Guang-yuan XU, Zhuo ZHANG, et al. Mechanism of Salidroside in Improving Glucose and Lipid Metabolism in Liver of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 130-134. DOI： 10.13422/j.cnki.syfjx.20182124.

摘要

目的：

探讨红景天苷对糖尿病大鼠肝脏糖脂代谢作用机制。

方法：

6～7周龄雄性Zucker糖尿病（ZDF）（fa/fa）大鼠24只，按照血糖随机分为模型组，红景天苷低剂量组（0.05 g·kg

－1

），红景天苷高剂量组（0.1 g·kg

－1

），8只/组，另选同周龄ZDF （fa/+）大鼠8只为正常组，连续给药干预6周。实验结束后，检测大鼠空腹血糖（fasting blood glucose

FBG），甘油三酯（triglyceride

TG），总胆固醇（total cholesterol

TC），游离脂肪酸（free fatty acids

FFA），超氧化物酶歧化酶（superoxide dismutase

SOD），丙二醛（malondialdehyde

MDA），过氧化氢酶（catalase

CAT），血清胰岛素水平（fasting insulin

Fins），胰岛素抵抗指数（homeostasis model assessment insulin resistance

HOMA-IR）；蛋白免疫印迹法（Western blot）检测肝脏蛋白激酶样内质网激酶（PKR-like ER kinase

PERK），核转录因子2相关因子2（NF-E2-relatedfactor2，Nrf2），血红素氧化酶-1（heme oxidase-1，HO-1）蛋白表达。

结果：

与正常组比较，模型组大鼠FBG

Fins

HOMA-IR

FFA

MDA明显升高（

0.05，

0.01）；SOD

CAT明显降低（

0.05，

0.01）；肝脏p-PERK

Nrf2，HO-1蛋白表达明显降低（

0.05，

0.01）。与模型组比较，红景天苷高、低剂量组大鼠FBG

Fins

HOMA-IR

FFA

MDA明显降低（

0.05，

0.01）；SOD

CAT明显升高（

0.05，

0.01）；肝脏p-PERK

Nrf2，HO-1蛋白表达明显升高（

0.05，

0.01）。

结论：

红景天苷能够改善糖尿病大鼠糖脂水平、胰岛素抵抗，可能是通过上调p-PERK

Nrf2，HO-1蛋白表达，抑制氧化应激实现的。

Abstract

Objective:

To explore the mechanism of salidroside(SAL) in improving glucose and lipid metabolism in liver of diabetic rats.

Method:

According to blood glucose

24 male ZDF(fa/fa) rats of 6-7 week old were randomly divided into model group

SAL-low group (0.05 g·kg

-1

)

SAL-high group (0.1 g·kg

-1

)

8 rats in each group. Another 8 ZDF (fa/+ ) rats were selected as normal group. All the rats were administered continuously for 6 weeks. After experiment

fasting blood glucose(FBG)

triglyceride(TG)

total cholesterol(TC)

free fatty acids(FFA)

superoxide dismutase (SOD)

malondialdehyde (MDA)

catalase (CAT)

fasting insulin(Fins)

and homeostasis model assessment insulin resistance(HOMA-IR) were measured. Western blot was used to detect phosphorylated PKR-like ER kinase(p-PERK)

NF-E2-relatedfactor2(Nrf2)

heme oxidase-1(HO-1) protein expression levels in liver.

Result:

As compared with normal group

FBG

Fins

HOMA-IR

FFA

and MDA were increased significantly in model group (

0.05

0.01)

while SOD

CAT levels were significantly decreased (

0.05

0.01); the protein expression levels of p-PERK

Nrf2

and HO-1 in control group were significantly decreased in model group(

0.05

0.01). As compared with model group

FBG

Fins

HOMA-IR

FFA

and MDA levels were decreased significantly (

0.05

0.01)

while SOD and CAT levels were significantly increased in SAL low and high groups (

0.05

0.01); and the protein expression levels of p-PERK

Nrf2

and HO-1 in SAL group were significantly increased(

0.05

0.01).

Conclusion:

The Salidroside can improve metabolism of glucose and lipid

IR in diabetic rats

and the mechanism may be associated with inhabiting oxidative stress through the up-regulation of p-PERK

Nrf2

and HO-1 protein expression.

关键词

Keywords

references

Cho N H , Shaw J E , Karuranga S ,et al . IDF diabetes atlas: global estimates of diabetes prevalence for 2017 and projections for 2045 [J]. Diabetes Res Clin Pract , 2018 , 138 ( 2 ): 271 - 281 .

Hajifaraji M , Jahanjou F , Abbasalizadeh F , et al . Effect of probiotic supplements in women with gestational diabetes mellitus on inflammation and oxidative stress biomarkers: a randomized clinical trial [J]. Asia Pac J Clin Nutr , 2018 , 27 ( 3 ): 581 - 591 .

王利民，池文婕，王玮，等 . 2型糖尿病患者空腹血糖、血脂、APN及VEGF与氧化应激的相关性 [J]．贵州医科大学学报， 2018 ， 43 ( 1 ): 61 - 66 .

Yamamoto M , Kensler T W , Motohashi H. The KEAP1-NRF2 system: a thiol-based sensor-effector apparatus for maintaining redox homeostasis [J]. Physiol Rev , 2018 , 98 ( 3 ): 1169 - 1203 .

Kumar J , Teoh S L , Das S , et al . Oxidative stress in oral diseases: understanding its relation with other systemic diseases [J]. Front Physiol , 2017 , 14 ( 8 ): 693 .

姜国哲，全贞玉，金润浩，等．红景天和轮叶党参混合提取物戊糖乳杆菌发酵条件及抗氧化作用分析 [J]．食品科学， 2018 ， 39 ( 2 ): 124 - 130 .

杨逸成，吕泰标，梁栋国，等．红景天苷干预疾病的实验研究进展 [J]．医学综述， 2018 ， 24 ( 2 ): 371 - 377 .

ZHENG T , YANG X , WU D , et al . Salidroside ameliorates insulin resistance through activation of a mitochondria-associated AMPK/PI3K/Akt/GSK3 β pathway [J]. Br J Pharmacol , 2015 , 172 ( 13 ): 3284 - 3301 .

Lee S Y , LAI F Y , SHI L S , et al . Rhodiola crenulata extract suppresses hepatic gluconeogenesis via activation of the AMPK pathway [J]. Phytomedicine , 2015 , 22 ( 4 ): 477 - 86 .

CHEN L , LIU P , FENG X , et al . Salidroside suppressing LPS-induced myocardial injury by inhibiting ROS-mediated PI3K/Akt/mTOR pathway in vitro and in vivo [J]. J Cell Mol Med , 2017 , 21 ( 12 ): 3178 - 3189 .

朴敏虎，王程瑜，李香丹，等．红景天苷对糖尿病肾病大鼠的治疗作用及其机制探讨 [J]．山东医药， 2017 ， 57 ( 10 ): 34 - 36 .

黄链莎，刘铜华，孙文，等．桂皮醛对糖尿病小鼠血糖水平的影响及机制 [J]．中国实验方学杂志， 2018 ， 24 ( 10 ): 95 - 100 .

穆晓红，刘铜华，孙文，等．糖痹康对高糖培养雪旺细胞自噬相关蛋白的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 10 ): 90 - 94 .

黄链莎，刘铜华，孙文，等．芹菜素对肥胖型小鼠脂肪组织AMPK信号通路的作用机制 [J]．中国实验方剂学杂志， 2018 ， 24 ( 10 ): 107 - 111 .

许光远，孙文，郭璇，等．青钱柳总皂苷对游离脂肪酸诱导的H4-ⅡE细胞脂肪代谢的影响及作用机制 [J]．中国实验方剂学杂志， 2017 ， 23 ( 15 ): 124 - 129 .

吴莉娟，孙文，吴丽丽，等．积雪草醇提物对2型糖尿病ZDF大鼠肝脏胰岛素抵抗的影响 [J]．中国实验方剂学杂志， 2017 ， 23 ( 10 ): 104 - 110 .

NI G L , CUI R , SHAO A M , et al . Salidroside ameliorates diabetic neuropathic pain in rats by inhibiting neuroinflammation [J]. J Mol Neurosci , 2017 , 63 ( 1 ): 9 - 16 .

LI F , TANG H , XIAO F , et al . Protective effect of salidroside from Rhodiolae Radix on diabetes-induced oxidative stress in mice [J]. Molecules , 2011 , 16 ( 12 ): 9912 - 9924 .

宋向军，陈晨．红景天苷对链脲佐菌素诱导糖尿病大鼠血糖、血脂和抗氧化能力的影响 [J]．现代药物与临床， 2015 ， 30 ( 8 ): 933 - 937 .

Al-Rashed F , Calay D , Lang M , et al . Celecoxib exerts protective effects in the vascular endothelium via COX-2-independent activation of AMPK-CREB-Nrf2 signalling [J]. Sci Rep , 2018 , 8 ( 1 ): 6271 .

俞天虹，朱琳，赵蓉，等．腺苷酸蛋白激酶活化在红景天苷抑制同型半胱氨酸诱导的人脐静脉内皮细胞内质网应激中的作用 [J]．中华中医药学刊， 2017 ， 35 ( 10 ): 2558 - 2562 .

Sharma R S , Harrison D J , Kisielewski D , et al . Experimental nonalcoholic steatohepatitis and liver fibrosis are ameliorated by pharmacologic activation of Nrf2 (NF-E2 p45-related factor 2) [J]. Cell Mol Gastroenterol Hepatol , 2017 , 5 ( 3 ): 367 - 398 .

Bellezza I , Giambanco I , Minelli A , et al . Nrf2-Keap1 signaling in oxidative and reductive stress [J]. Biochim Biophys Acta , 2018 , 1865 ( 5 ): 721 - 733 .

李涛，张伟，赵俊杰，等．红景天苷通过Nrf2/HO-1通路减轻6-OHDA诱导的SH-SY5Y细胞损伤 [J]．神经解剖学杂志， 2016 ， 32 ( 6 ): 751 - 756 .

Draganski A , Tar M T , Villegas G , et al . Topically applied curcumin-loaded nanoparticles treat erectile dysfunction in a rat model of type-2 diabetes [J]. J Sex Med , 2018 , 15 ( 5 ): 645 - 653 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research Progress on Protective Effect and Mechanism of Salidroside on Cerebral Ischemia Injury： A Review

Protective Effect of Guilu Erxianjiao on Reproductive Injury in Diabetic Rats and Its Mechanism

Liuwei Dihuangtang Alleviates Myelin Injury in vHIP and Depression-like Behavior of Diabetes Mellitus Combined with Comorbid Depression Rats via AMPK/Akt/GSK3β/Nrf2 Pathway

Progress on Hypoglycemic Effect and Mechanism of Momordica charantia

Experimental Study of Reversion of Puerariae Radix Lobatae Flavonoids on Pancreas Injury Induced by Streptozotocin in Mice

Related Author

XU Haoqun

ZHANG Wenjing

LOU Yuan

JIANG Yanan

YUAN Xin

WANG Yuan

Baowei LU

Lumei LIU

Related Institution

Shandong University of Traditional Chinese Medicine（TCM）

Innovation Center of Classic TCM Formula

Hunan University of Chinese Medicine

Affiliated Bao'an Hospital of Traditional Chinese Medicine，The Seventh Clinical Medical College， Guangzhou University of Traditional Chinese Medicine， henzhen

Hunan University of Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰