Effect of Whole Prescription and Decomposed Recipes of Tiaomai Drink on mRNA Expression of L-type Calcium Channels in Tachyarrthythmia Model of Big Rats

HAN Yao; QIN Tian-yu; LIU Hong-xu; SUN Wen; DAI Mei; ZHANG Da-wei; WEI Zhi-zhen

doi:10.13422/j.cnki.syfjx.20182143

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Effect of Whole Prescription and Decomposed Recipes of Tiaomai Drink on mRNA Expression of L-type Calcium Channels in Tachyarrthythmia Model of Big Rats

更新时间：2024-01-04

- Effect of Whole Prescription and Decomposed Recipes of Tiaomai Drink on mRNA Expression of L-type Calcium Channels in Tachyarrthythmia Model of Big Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 20, Pages: 123-128(2018)
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- DOI：10.13422/j.cnki.syfjx.20182143
  CLC： R285.5
- Published：2018
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HAN Yao, QIN Tian-yu, LIU Hong-xu, et al. Effect of Whole Prescription and Decomposed Recipes of Tiaomai Drink on mRNA Expression of L-type Calcium Channels in Tachyarrthythmia Model of Big Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 123-128.
DOI：

HAN Yao, QIN Tian-yu, LIU Hong-xu, et al. Effect of Whole Prescription and Decomposed Recipes of Tiaomai Drink on mRNA Expression of L-type Calcium Channels in Tachyarrthythmia Model of Big Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 123-128. DOI： 10.13422/j.cnki.syfjx.20182143.

摘要

目的：观察调脉饮全方及其拆方对快速性心律失常模型大鼠心电图及心室肌L型钙通道（L-type calcium channels，LTCC）α1c亚基mRNA表达的影响。方法：把60只Wistar大鼠随机分成6组，每组各10只，分别为模型组，调脉饮全方组（22 g·kg-1），凉血清热组（7 g·kg-1），行气通脉组（7.5 g·kg-1），益气养心组（7.5 g·kg-1）和西药组（胺碘酮，100 mg·kg-1）。除模型组外，每组按相应剂量分别灌胃给药7 d。采用乌头碱舌下静脉注射造模，分别记录室性早搏、室性心动过速和室颤及死亡出现的时间，通过实时荧光定量聚合酶链式反应（Real-time PCR）检测和蛋白免疫印迹法（Western blot）测定各组心室肌组织LTCCα1c亚基mRNA和蛋白表达情况。结果：与模型组比较，全方组、凉血清热组、胺碘酮组大鼠出现室性早搏时间均有延迟，其中全方组、胺碘酮组大鼠出现室性早搏时间延迟明显（P<0.05，P<0.01）；全方组、凉血清热组、胺碘酮组出现室性心动过速时间明显延迟（P<0.05）；全方组、凉血清热组、胺碘酮组出现室颤时间明显延迟（P<0.05，P<0.01）；全方组、凉血清热组、胺碘酮组出现死亡时间明显延迟（P<0.05，P<0.01）。与模型组相比，全方组、凉血清热组、胺碘酮组大鼠心肌LTCCα1c亚基mRNA表达均有所降低，其中全方组和胺碘酮组与模型组相比有显著差异（P<0.05）；与模型组比较，全方组、凉血清热组、胺碘酮组大鼠心肌LTCCα1c亚基蛋白表达均有所降低，有显著差异（P<0.01）。结论：调脉饮全方及其中凉血清热药具有抗实验性快速心律失常的作用，作用机制可能与干预心肌组织LTCC mRNA的表达水平有关。

Abstract

Objective: To observe the effect of the whole prescription and decomposed recipes of Tiaomai drink on the electrocardiogram changes of experimental tachyarrthythmia rats and the mRNA expression levels of α1c sub-units in L-type calcium channels (LTCC) of myocardium.Method: The 60 Wistar rats were randomly divided into the model group

Tiaomai drink whole prescription group (22 g·kg-1)

blood cooling and heat clearing medicine group (7 g·kg-1)

Qi-regulating and blood flow promoting medicine group (7.5 g·kg-1)

Qi and heart tonifying medicine group (7.5 g·kg-1)

and western medicine group (amiodarone

100 mg·kg-1). Except those in model group

corresponding dose of medicines was given in other groups for 7 days. Sublingual intravenous injection of aconitine was used for modeling

and then the time to have ventricular premature beat

ventricular tachycardia

ventricular fibrillation and death was recorded respectively. Quantitative Real-time polymerase chain reaction (Real-time PCR) and Western blot were respectively used to detect the mRNA and protein expression levels of LTCCα1c sub-units in rat myocardial tissue.Result: As compared with the model group

there was a delay in ventricular premature beats in the whole prescription group

blood cooling group and the western medicine group

among which the time delay of ventricular premature beat was obvious in the whole prescription group and the western medicine group (P<0.005

P<0.01); the time of ventricular tachycardia was significantly delayed in the whole prescription group

blood cooling group and western medicine group (P<0.05); the time of ventricular fibrillation was significantly delayed in the whole prescription group

blood cooling group and western medicine group (P<0.05

P<0.01); the death time was significantly delayed in the whole prescription group

blood cooling group and western medicine group (P<0.05

P<0.01). As compared with the model group

the mRNA expression of myocardial LTCCα1c was decreased in the whole prescription group

blood cooling group and the western medicine group

and the whole prescription group and the western medicine group were significantly different from the model group (P<0.01); the protein expression of myocardial LTCCα1c was significantly decreased in the whole prescription group

blood cooling group and the western medicine group.Conclusion: The whole prescription as well as blood cooling and heat clearing medicine were effective for experimental tachyarrhythmia

and the mechanism may be related to the intervention on the mRNA expression level of the myocardial tissue L-type calcium channel.

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