Objective: To analyze the anthocyanins in 12 varieties of blueberries by ultra performance liquid chromatography-tandem triple-quadrupole mass spectrometry (UPLC-MS/MS). Method: The 80% ethanol was used as solvent to extract anthocyanin from blueberry under assistance of ultrasound

and the centrifugated supernatant of the extract was treated in a rotating evaporator and concentrated in 6% formic acid solution to the scale line. After ultrafiltration

the extract anthocyanin was analyzed on ACQUITY UPLC BEH C18 column (2.1 mm×50 mm

1.7 μm)

with 6% formic acid and acetonitrile as the mobile phase for gradient elution. The positive ion mode of electrospray ionization(ESI) was adopted in the course of multiple reactions monitoring (MRM). Result: The anthocyanins of 12 varieties were similar. R26 blueberry had the most abundant anthocyanin species (23 species). N15 blueberry had the least anthocyanin species (20 species). Delphinidin and malvidin were the two main types of anthocyanins in blueberries. The total anthocyanins content was 820.43-2 114.627 μg·g-1 in 12 varieties

with significant differences

R26 > N12 > S28 > R6 > S30 > S7-1 > S12 > S7-2 > S7-3 > N15 > N1 > N14 from high to low. The total anthocyanins content in blueberrys of R series was higher than that in blueberrys of S series. The anothcyanins content in blueberrys of N series was lowest. There were also significant differences in contents of anthocyanins between different varieties of blueberries. Conclusion: The content of total anthocyanins in the 12 varieties of blueberries detected in the experiment showed significant differences

which could provide basic data for the development of health products and drugs with blueberry anthocyanin as a functional component

and also provide reference for the activity test of blueberry anthocyanin as well as the detection and analysis of blueberry anthocyanin.