Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos

Qin-rong ZHOU; Pan-pan SUN; Lin-yan XIAO; Qi-nan LIU; Li ZHANG

doi:10.13422/j.cnki.syfjx.20182203

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Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos

Pharmacy and Processing | 更新时间：2021-02-09

- Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 2, Pages: 35-39(2019)
- 作者机构：
  
  南京中医药大学　江苏省中药资源产业化过程协同创新中心，中药资源产业化与方剂创新药物国家地方联合工程研究中心，南京　 210023
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182203
  CLC： R22; R24; R28; R943.1
- Received：17 March 2018，
  
  Published Online：28 August 2018，
  
  Published：20 January 2019
- 稿件说明：
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Qin-rong ZHOU, Pan-pan SUN, Lin-yan XIAO, et al. Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 35-39.
DOI：

Qin-rong ZHOU, Pan-pan SUN, Lin-yan XIAO, et al. Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 35-39. DOI： 10.13422/j.cnki.syfjx.20182203.

摘要

目的：

比较醋炙前后芫花二氯甲烷提取物对于大鼠正常肝细胞BRL及大鼠正常肾细胞NRK的细胞毒性变化。

方法：

选择BRL及NRK细胞为研究对象，采用噻唑蓝(MTT)比色法评价生、醋芫花二氯甲烷部位对BRL与NRK活性的影响；测定NRK细胞中尿素氮(BUN)，乳酸脱氢酶(LDH)，谷胱甘肽(GSH)的含量，以及BRL细胞中丙氨酸氨基转移酶(ALT)，天门冬氨酸氨基转移酶(AST)，碱性磷酸酶(ALP)，LDH，GSH的含量，用于评价生、醋芫花二氯甲烷部位对BRL和NRK的氧化损伤作用。

结果：

与空白组相比，生芫花二氯甲烷部位对NRK和BRL细胞具有显著毒性，显著提高NRK细胞中BUN和LDH的含量(

0.05，

0.01)；显著提高BRL细胞中AST，ALT，ALP和LDH的含量(

0.05，

0.01)；降低NRK和BRL细胞中的GSH含量。与生芫花二氯甲烷部位相应剂量组相比，醋芫花二氯甲烷部位相应剂量组能提高NRK和BRL的细胞活性，降低NRK细胞中BUN和LDH的含量，降低BRL细胞中ALT，AST，ALP和LDH的含量；提高NRK和BRL细胞中GSH的含量。

结论：

醋炙可降低芫花二氯甲烷部位对大鼠肝肾细胞的毒性，提高大鼠肝肾细胞功能与抗氧化能力。

Abstract

Objective:

To campare the hepatotoxicity on BRL and nephrotoxicity on NRK caused by dichloromethane site of Genkwa Flos before and after being processed with vinegar.

Method:

BRL of normal hepatocytes and NRK of normal renal cells in rats were selected as the subjects.Thiazolyl blue tetrazolium bromide method(MTT) was adopted to evaluate the effect of dichloromethane sites of raw and vinegar-processed products on cell activity of NRK and BRL.The levels or contents of aspartate aminotransferase(AST)

alanine aminotransferase(ALT)

alkaline phosphatase(ALP)

glutathione(GSH)

lactate dehydrogenase(LDH)

blood urea nitrogen(BUN) were determined in cell culture supernatant and splitting supernatant for evaluation of their oxidative damage effect.

Result:

Compared with the blank group

dichloromethane site of raw products could obviously inhibit the cell activity of NRK and BRL

and increase the levels of AST

ALT

ALP and LDH(

0.05

0.01) in BRL and decrease the content of GSH; meanwhile

it could obviously increase the levels of LDH and BUN(

0.05

0.01) and decrease the content of GSH in NRK.Compared with corresponding dose group of raw products

vinegar-processed products could improve cell activity of NRK and BRL

and decrease the levels of AST

ALT

ALP and LDH in BRL and increase the content of GSH; also

it decreased the levels of LDH and BUN and increased the content of GSH in NRK.

Conclusion:

Processing with vinegar can attenuate the hepatotoxicity and nephrotoxicity on NRK and BRL caused by dichloromethane site of Genkwa Flos

it can improve hepatic and renal function and antioxidant capacity.

关键词

Keywords

references

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