Overall Evaluation on Mutual Detoxication Mechanism of Tripterygii Radix et  Rhizoma and Lysimachiae Herba by Correlation Analysis of “Chemical Composition  Spectrum-attenuation Spectrum-biological Information Spectrum”

Jun-ming WANG; Jin-hua LI; Hong CAI; Jin-yang LI; Yue-yue ZHANG; Jun LI; Ying CUI

doi:10.13422/j.cnki.syfjx.20182206

您当前的位置：

首页 >

文章列表页 >

Overall Evaluation on Mutual Detoxication Mechanism of Tripterygii Radix et Rhizoma and Lysimachiae Herba by Correlation Analysis of “Chemical Composition Spectrum-attenuation Spectrum-biological Information Spectrum”

Compatibility | 更新时间：2021-02-09

- Overall Evaluation on Mutual Detoxication Mechanism of Tripterygii Radix et Rhizoma and Lysimachiae Herba by Correlation Analysis of “Chemical Composition Spectrum-attenuation Spectrum-biological Information Spectrum”
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 3, Pages: 15-20(2019)
- 作者机构：
  
  河南中医药大学　呼吸疾病诊疗与新药研发河南省协同创新中心，药学院，科研实验中心，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182206
  CLC： R22;R24;R28;C37;R943.1
- Received：25 March 2018，
  
  Published Online：28 August 2018，
  
  Published：05 February 2019
- 稿件说明：
移动端阅览
Jun-ming WANG, Jin-hua LI, Hong CAI, et al. Overall Evaluation on Mutual Detoxication Mechanism of Tripterygii Radix et Rhizoma and Lysimachiae Herba by Correlation Analysis of “Chemical Composition Spectrum-attenuation Spectrum-biological Information Spectrum”[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(3): 15-20.
DOI：

Jun-ming WANG, Jin-hua LI, Hong CAI, et al. Overall Evaluation on Mutual Detoxication Mechanism of Tripterygii Radix et Rhizoma and Lysimachiae Herba by Correlation Analysis of “Chemical Composition Spectrum-attenuation Spectrum-biological Information Spectrum”[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(3): 15-20. DOI： 10.13422/j.cnki.syfjx.20182206.

摘要

目的:

整体评价雷公藤(LGT)配伍金钱草(JQC)后在荷瘤状态下的相杀减毒作用机制。

方法:

以配伍前后12个差异特征成分为化学成分谱，以丙氨酸氨基转移酶(ALT)，天门冬氨酸氨基转移酶(AST)，肌酐(Cr)和尿素氮(BUN)，肝丙二醛(MDA)，肾MDA共6指标为减毒作用谱，以肝和肾的谷胱甘肽(GSH)，谷胱甘肽-

-转移酶(GST)，谷胱甘肽过氧化物酶(GPx)，超氧化物歧化酶(SOD)，过氧化氢酶(CAT)，白细胞介素(IL)-10共12个生物指标作为生物信息谱，通过主成分分析(PCA)整体评价LGT-JQC在荷瘤S180和H22肿瘤状态下的相杀减毒作用及其机制，通过对“化学成分谱-减毒作用谱-生物信息谱”灰色关联分析(GCA)评价化学成分和生物指标对其相杀减毒作用的贡献大小。

结果:

与模型组比较，LGT单用后使S180和H22的减毒作用谱得分

值和

值均显著升高(

0.01)；与LGT单用组比较，LGT-JQC在质量配比为4∶1，2∶1，1∶1，1∶2，1∶4配伍时均能显著降低LGT引起的过高水平的

值和

值(

0.01)；LGT-JQC在质量比为4∶1，1∶1，1∶2，1∶4配伍时的

值和

值均明显高于质量比在2∶1时相应的

值和

值(

0.01)。LGT能使S180和H22荷瘤状态下生物信息谱得分

值和

值均显著降低，配伍JQC可使

值和

值显著升高。对二者在S180和H22肿瘤状态下相杀减毒作用贡献最大的化学成分分别为3#和10#特征成分，贡献最大的生物指标分别为肾GPx和肾GSH。

结论:

LGT和JQC在质量比为4∶1~1∶4配伍时，对LGT所致的荷瘤S180和H22肿瘤状态下的毒性均有减毒作用，二者相杀减毒的最佳配比均为2∶1，减毒作用体现了中医“有故无殒”思想，其减毒作用机制涉及肝、肾的抗氧化损伤及抗炎症反应，其中尤以肾GPx(S180)和肾GSH(H22)对减毒作用机制的贡献最大.

Abstract

Objective:

To overall evaluate the mutual detoxication mechanism of Tripterygii Radix et Rhizoma(LGT) compatible with Lysimachiae Herba(JQC) in tumor-bearing state.

Method:

Twelve differentially characteristic components before and after compatibility were used as chemical composition spectrum

six indicators including serum alanine aminotransferase(ALT)

aspartate aminotransferase(AST)

creatinine(Cr) and urea nitrogen(BUN)

malondialdehyde(MDA) levels in the liver and kidney tissues were used as attenuation spectrum

and twelve biological indicators including glutathione(GSH)

glutathione-

-transferase(GST)

glutathione peroxidase(GPx)

superoxide dismutase(SOD)

catalase(CAT) and interleukin(IL)-10 in the liver and kidney were used as the biological information spectrum.Mutual detoxication mechanisms of LGT compatible with JQC in tumor-bearing state were overall evaluated by principal component analysis(PCA)

and the contribution of chemical components and biological indicators to mutual detoxication was further evaluated by gray correlation analysis(GCA) of“chemical composition spectrum-attenuation spectrum-biological information spectrum”.

Result:

Compared with the model group

the attenuation spectrum scores

values of S180(

value) and H22(

value) increased significantly after LGT being used alone(

0.01). Compared with LGT alone group

the compatibility groups of LGT and JQC significantly reduced excessive

value and

value caused by LGT when the ratio of LGT and JQC was 4∶1

2∶1

1∶1

1∶2 and 1∶4(

0.01). The overall efficacy of

values(

value and

value) of LGT-JQC in the mass ratios including 4∶1

1∶1

1∶2 and 1∶4 was significantly higher than that in the ratio of 2∶1(

0.01). LGT also caused a significant decrease in the

values of the bioinformatics scores in the S180(

value) and H22(

value) tumor-bearing state

these two values were significantly increased after compatibility with JQC.The chemical components contributing the most to the attenuating effect of S180 and H22 in tumor-bearing state were 3# and 10#

respectively.The most important biological indicators were kidney GPx and renal GSH.

Conclusion:

LGT combined with JQC in the mass ratio of 4∶1-1∶4 can attenuate LGT-induced subacute toxicity in S180 and H22 tumor-bearing state

and the best ratio of such effect is 2∶1.The attenuating effect reflects the thought of“there is no reason why there is no meteorology”. The mechanism of attenuating action involves antioxidative damage and anti-inflammatory reaction of the liver and kidney

especially the renal GPx(S180) and renal GSH(H22) as the greatest contribution to the detoxication mechanism.

关键词

Keywords

references

刘莹，仲青香，邱辉辉，等．基于肝毒性的雷公藤中药复方配伍减毒的研究进展 [J]. 中国中药杂志， 2017 , 42 ( 16 ): 3044 - 3048 .

CHAN S F , CHEN Y Y , LIN J J , et al . Triptolide induced cell death through apoptosis and autophagy in murine leukemia WEHI-3 cells in vitro and promoting immune responses in WEHI-3 generated leukemia mice in vivo [J]. Environ Toxicol , 2017 , 32 ( 2 ): 550 - 568 .

CHANG H J , Kim M H , Baek M K , et al . Triptolide inhibits tumor promoter-induced uPAR expression via blocking NF- к B signaling in human gastric AGS cells [J]. Anticancer Res , 2007 , 27 ( 5A ): 3411 - 3417 .

Kim M J , Lee T H , Kim S H , et al . Triptolide inactivates Akt and induces caspase-dependent death in cervical cancer cells via the mitochondrial pathway [J]. Int J Oncol , 2010 , 37 ( 5 ): 1177 - 1185 .

LIU J , JIANG Z , XIAO J , et al . Effects of triptolide from Tripterygium wilfordii on ER α and p53 expression in two human breast cancer cell lines [J]. Phytomedicine , 2009 , 16 ( 11 ): 1006 - 1013 .

XIE C Q , ZHOU P , ZUO J , et al . Triptolide exerts pro-apoptotic and cell cycle arrest activity on drug-resistant human lung cancer A549/Taxol cells via modulation of MAPK and PI3K/Akt signaling pathways [J]. Oncol Lett , 2016 , 12 ( 5 ): 3586 - 3590 .

WANG Z , Ravula R , SHI L , et al . Overcoming chemoresistance in prostate cancer with Chinese medicine Tripterygium wilfordii via multiple mechanisms [J]. Oncotarget , 2016 , 7 ( 38 ): 61246 - 61261 .

BAO J , DAI S M. A Chinese herb Tripterygium wilfordii Hook F in the treatment of rheumatoid arthritis: mechanism, efficacy, and safety [J]. Rheumatol Int , 2011 , 31 ( 9 ): 1123 - 1129 .

HUANG L , FENG S , WANG H. Decreased bone mineral density in female patients with systemic lupus erythematosus after long-term administration of Tripterygium wilfordii Hook. F. [J]. Chin Med J (Engl) ， 2000 , 113 ( 2 ): 159 - 161 .

WAN Y G , ZHAO Q , SUN W , et al . Contrasting dose-effects of multi-glycoside of Tripterygium wilfordii HOOK. f. on glomerular inflammation and hepatic damage in two types of anti-Thy1.1 glomerulonephritis [J]. J Pharmacol Sci , 2012 , 118 ( 4 ): 433 - 446 .

LI X X , DU F Y , LIU H X , et al . Investigation of the active components in Tripterygium wilfordii leading to its acute hepatotoxicty and nephrotoxicity [J]. J Ethnopharmacol , 2015 , 162 : 238 - 243 .

钟赣生．中药学 [M]. 北京：中国中医药出版社， 2016 ： 38 .

丁中连．金钱草的药用小方 [J]. 农村百事通， 2010 ( 2 ): 74 - 75 .

王君明，李金花，蔡泓，等．基于主成分分析和灰色关联分析方法评价雷公藤配伍金钱草的相杀减毒作用的化学基础 [J]. 中国全科医学， 2018 , 21 ( 21 ): 2622 - 2626 .

王君明，李金花，李金洋，等．基于主成分分析评价雷公藤配伍金钱草的相杀减毒作用 [J]. 中国实验方剂学杂志， 2018 , 24 ( 21 ): 1 - 6 .

Högberg J , Larson R E , Kristoferson A , et al . NADPH-dependent reductase solubilized from microsomes by peroxidation and its activity [J]. Biochem Biophys Res Commun , 1974 , 56 ( 3 ): 836 - 842 .

于海帅．基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索 [J]. 中国实验方剂学杂志， 2016 , 22 ( 21 ): 27 - 31 .

罗益远，刘娟秀，刘训红，等．不同加工方法何首乌中多元功效物质的测定及主成分分析 [J]. 中草药， 2016 , 47 ( 2 ): 318 - 323 .

梁健钦，王剑，熊万娜，等．基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系 [J]. 中国实验方剂学杂志， 2015 , 21 ( 1 ): 121 - 125 .

刘维，张祎楠，裴瑾，等．川牛膝品种与品质的灰色关联度分析研究 [J]. 中国药学杂志， 2014 , 49 ( 20 ): 1796 - 1801 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Evaluation on Mutual Detoxication of Compatibility of Tripterygii Radix et Rhizoma and Lysimachiae Herba Based on Principal Component Analysis

Meridian Tropism of Components in Bupleuri Radix Based on Nonalcoholic Steatohepatitis Model and Principal Component Analysis

Quality Evaluation of Aurantii Fructus Based on Fingerprint Qualitative Analysis， Multi-component Quantitative Analysis and Chemometrics

Prediction of Disintegration Time of Traditional Chinese Medicine Tablets Based on Generalized Regression Neural Network

Relationship Between Syndrome Elements and Therapy of Ischemic Stroke Based on Scale Hierarchy and Principal Component Analysis

Related Author

WANG Jun-ming

LI Jin-hua

LI Jin-yang

CAI Hong

ZHANG Yue-yue

RONG Chun-lei

LI Jun

CUI Ying

Related Institution

Key Laboratory of Basic and Application Research of Beiyao，Ministry of Education， Key Laboratory of Traditional Chinese Medicine （TCM） Pharmacodynamic Material Base， Heilongjiang University of Chinese Medicine

School of TCM， Guangdong Pharmaceutical University

Key Laboratory of Modern Preparation of Traditional Chinese Medicine，Ministry of Education，Jiangxi University of Chinese Medicine

The First Affiliated Hospital of Nanchang University

Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique， Ministry of Education，Tianjin University of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰