TANG Wei-wei, WANG Yan-hui, LEI Jing-wei, et al. Fingerprints of Gardeniae Fructus with Different Fruit Shapes[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 70-76.
DOI:
TANG Wei-wei, WANG Yan-hui, LEI Jing-wei, et al. Fingerprints of Gardeniae Fructus with Different Fruit Shapes[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 70-76. DOI: 10.13422/j.cnki.syfjx.20182208.
Fingerprints of Gardeniae Fructus with Different Fruit Shapes
Objective:To compare the high performance liquid chromatography (HPLC) and infrared spectroscopy (IR) fingerprints of different fruit-shaped Gardenia jasminoides and analyze their differences. Method:The 48 batches of Gardeniae Fructus samples were processed to establish HPLC and IR fingerprints
and then the similarity evaluation and principal components cluster analysis were performed on Fujian
Henan
and Jiangxi six and seven edges Gardeniae Fructus samples. Result:HPLC fingerprints:22 common peaks were identified. The similarity was ranged from 0.789 to 0.896 among 6 edges Gardeniae Fructus in 48 samples
and 0.598 to 0.103 among seven edges Gardeniae Fructus. The similarity was low between six and seven edges Gardeniae Fructus in fruit shape
with great difference in components. In the principal components and cluster analysis
the six and seven edges Gardeniae Fructus were divided into two categories:Fujian six edges and Henan six edges Gardeniae Fructus were classified into one same category; Henan seven edges Gardeniae Fructus and Jiangxi seven edges Gardeniae Fructus were classified into one same category; the same fruit shape in different places was classified into one category. Infrared fingerprinting:In the cluster analysis
when the between-class distance was 1.35
Gardeniae Fructus samples were divided into 2 categories
six edges Gardeniae Fructus and seven edges Gardeniae Fructus; when the between-class distance was 0.875
Fujian 6edges Gardeniae Fructus and Henan six edges Gardeniae Fructus were classified into one category;when the between-class distance was 0.756
Henan seven edges Gardeniae Fructus and Jiangxi seven edges Gardeniae Fructus were classified into one category. Conclusion:This study shows that the contained components are obviously different in different fruit shaped Gardeniae Fructus
and the fingerprinting analysis can be used to evaluate different fruit shaped Gardeniae Fructus materials
providing reference for the clinical application of different fruit shaped Gardeniae Fructus in traditional Chinese medicine.