Analysis of Zingiberaceae in E'mei Area Using ITS2 Sequences

Yao-yao REN; Nan-ping JIANG; Liang ZHANG; Liang-ke SONG; Rui-ying LIU; Rui TAN

doi:10.13422/j.cnki.syfjx.20182209

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Analysis of Zingiberaceae in E'mei Area Using ITS2 Sequences

Resource and Quality Evaluation | 更新时间：2021-02-09

- Analysis of Zingiberaceae in E'mei Area Using ITS2 Sequences
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 217-223(2019)
- 作者机构：
  
  西南交通大学　生命科学与工程学院，成都　 610031
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182209
  CLC： R284.1;R282.5;R289;R931.5
- Received：20 May 2018，
  
  Published Online：31 August 2018，
  
  Published：05 January 2019
- 稿件说明：
移动端阅览
Yao-yao REN, Nan-ping JIANG, Liang ZHANG, et al. Analysis of Zingiberaceae in E'mei Area Using ITS2 Sequences[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 217-223.
DOI：

Yao-yao REN, Nan-ping JIANG, Liang ZHANG, et al. Analysis of Zingiberaceae in E'mei Area Using ITS2 Sequences[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 217-223. DOI： 10.13422/j.cnki.syfjx.20182209.

摘要

目的：

评价以ITS2序列作为DNA条形码对峨眉山地区姜科药用植物的鉴定。

方法：

于四川省峨眉山地区采集姜科药用植物样本43个，提取其基因组DNA，进行转录间隔区（ITS）2序列PCR扩增、测序，从GenBank上下载40条姜科植物ITS2序列；运用MEGA6.0软件对所有样本序列种间和种内遗传距离进行计算分析，构建neighbor joining（NJ）系统进化树；利用TAXON DNA 软件分析序列种内、种间变异并作 barcoding gap 分析；从ITS2数据库中预测并比较样本ITS2序列的二级结构差异。

结果：

姜科样本种间最小距离大于种内最大距离，有较为明显的barcoding gap；NJ进化树中各属样本分别聚山姜属（

Alpinia

），姜黄属（

Curcuma

），舞花姜属（

Globba

），姜花属（

Hedychium

），姜属（

Zingiber

）五大支，种内之间于各属分支中各聚为小支；各属及各种样本ITS2二级结构存在差异明显。

结论：

以ITS2序列作为DNA条形码，能够对姜科药用植物进行准确、快速的识别和鉴定，准确地弄清物种之间的系统进化关系，为该属药用植物的分布、种群及种群量研究提供指导，对其质量控制、安全用药及资源保护及合理开发利用提供理论依据。

Abstract

Objective:

To evaluate the use of ITS2 sequences as DNA barcode to identify the Zingiberaceae medicinal plants from E'mei area.

Method:

The genomic DNAs were extracted from 43 Zingiberaceae medicinal plant samples from Sichuan E'mei area. The ITS2 sequences of these samples were amplified and bidirectionally sequenced by PCR. 40 ITS2 sequences were downloaded from the GenBank

and then the interspecific and intraspecific genetic distances were calculated and analyzed by using MEGA 6.0 to construct Neighbor-joining (NJ) tree; TAXON DNA software was also used to analyze intraspecific and interspecific variations and barcoding gaps. The differences in secondary structure of the ITS2 sequences were predicted and compared.

Result:

The minimum interspecific distance in Zingiberaceae samples was greater than the maximum intra specific distance

with obvious barcoding gap. The NJ tree showed that the samples were clustered into five different branches

Alpinia

Curcuma

Globba

Hedychium

and

Zingiber

respectively

and further cluster into sub-branches. Significant differences were also present in the secondary structures of ITS2 between different samples.

Conclusion:

ITS2 sequences as DNA barcode can be used to conduct accurate and rapid identification of the Zingiberaceae plants and clearly figure out the phylogenetic relationship among them

providing guidance for the study of the distribution of medicinal plants of this genus

as well as theoretical basis for the quality control

medication safety and rational development of Zingiberaceae medicinal plants in E'mei area.

关键词

Keywords

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