Anti-atherosclerosis Mechanism of Sanhuang Xiexintang in Activating Blood and Resolving Stasis Formula

YIN Xiao-jie; MA Xiao-jing; WANG Lan; GONG Lei-lei; FAN Mo-qi; LI Li; XIAO Yong-qing; LIANG Ri-xin; WANG Yan-li

doi:10.13422/j.cnki.syfjx.20182215

您当前的位置：

首页 >

文章列表页 >

Anti-atherosclerosis Mechanism of Sanhuang Xiexintang in Activating Blood and Resolving Stasis Formula

更新时间：2024-09-23

- Anti-atherosclerosis Mechanism of Sanhuang Xiexintang in Activating Blood and Resolving Stasis Formula
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 22, Pages: 83-88(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182215
  CLC： R285
- Published：2018
- 稿件说明：
移动端阅览
YIN Xiao-jie, MA Xiao-jing, WANG Lan, et al. Anti-atherosclerosis Mechanism of Sanhuang Xiexintang in Activating Blood and Resolving Stasis Formula [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 83-88.
DOI：

YIN Xiao-jie, MA Xiao-jing, WANG Lan, et al. Anti-atherosclerosis Mechanism of Sanhuang Xiexintang in Activating Blood and Resolving Stasis Formula [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 83-88. DOI： 10.13422/j.cnki.syfjx.20182215.

摘要

目的：研究三黄泻心汤活血化瘀优势方对氧化低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞（HUVECs）损伤的影响并探讨其抗动脉粥样硬化的作用机制。方法：采用体外培养的HUVECs，分为空白组，ox-LDL损伤组（模型组），三黄泻心汤活血化瘀优势方15.63，31.25，62.50 mg·L-1组；用200 mg·L-1ox-LDL孵育24 h，造成HUVECs损伤模型；细胞存活率采用噻唑蓝（MTT）比色法测定，放射免疫法检测细胞上清液中内皮素（ET-1），6-酮-前列腺素F1α（6-keto-PGF1α），血栓烷素B2（TXB2）含量，半自动生化分析仪检测抗氧化应激指标超氧化物歧化酶（SOD），丙二醛（MDA），一氧化氮（NO）水平。采用试剂盒荧光定量法检测活性氧（ROS）含量，检测细胞间黏附分子-1（ICAM-1），血管细胞黏附分子-1（VCAM-1）及单核细胞趋化因子-1（MCP-1）水平酶联免疫吸附测定。细胞凋亡率及线粒体膜电位变化采用流式细胞术，采用蛋白免疫印迹法（Western blot）检测凋亡相关蛋白B淋巴细胞瘤-2（B-cell lymphoma-2，Bcl-2），Bcl-2相关X蛋白（Bcl-2-associated X protein，Bax），半胱氨酸蛋白酶-3（cysteine aspartate-specific protease-3，Caspase-3），Caspase-9表达。结果：与空白组比较，模型组HUVECs存活率降低；MDA，MCP-1，ICAM-1，VCAM-1的水平升高，SOD和NO含量降低，ROS的分泌增加，细胞凋亡率升高，Bax，Caspase-3和Caspase-9的表达量增加，Bcl-2的表达量减少（P<0.05，P<0.01）。与模型组比较，不同质量浓度的三黄泻心汤活血化瘀优势方使HUVECs存活率明显上升，显著降低MDA，MCP-1，ICAM-1，VCAM-1的水平；提高SOD和NO含量，且减少ROS的分泌，降低细胞凋亡率，降低Bax，Caspase-3和Caspase-9的表达量，增加Bcl-2的表达量（P<0.05，P<0.01）。结论：在ox-LDL诱导损伤的HUVECs模型上，三黄泻心汤活血化瘀优势方具有抑制细胞凋亡，减轻炎性反应和抗氧化的作用，可能与抗动脉粥样硬化有密切关系。

Abstract

Objective:To investigate the anti-atherosclerosis mechanism of Sanhuang Xiexintang in activating blood and resolving stasis (SXTAR) in vitro. Method:The cell injury model was established with oxidized law-density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells live (HUVECs) in vitro

and divided into control group

model group

and SXTAR 15.63

31.25

62.50 mg·L-1 groups. The HUVECs livability and the concerning markers in cell supernatant

like malondialdehyde(MDA)

monocyte chemoattractant protein-1(MCP-1)

intercellular adhesion molecule-1(ICAM-1)

vascular cell adhesion molecule-1(VCAM-1)

superoxide dismutase(SOD) and nitric oxide(NO)

were detected; reactive oxygen species(ROS) was measured by fluorescence quantitation. Apoptosis rate and mitochondrial membrane potential were determined by flow cytometry. Western blot was employed for testing expressions of B-cell lymphoma-2(Bcl-2)

Bcl-2 associated X protein(Bax)

cysteine aspartate-specific protease-3(Caspase-3). Result:The survival rate of HUVECs in model group was significantly lower than that of control group. In the model group

MDA

MCP-1

ICAM-1

VCAM-1 were significant increases

SOD and NO content were decreased

apoptosis rate

ROS and expressions of Bax

Caspase-3

Caspase-9 were up-regulated

and expression of Bcl-2 was down(P<0.05

P<0.01)-regulated. SXTAR significantly increased the survival rate of HUVECs. Compared with the model group

SXTAR significantly decreased contents of MDA

MCP-1

ICAM-1 and VCAM-1.SOD and NO content were increased

apoptosis rate

ROS and expressions of Bax

Caspase-3

Caspase-9 was down-regulated

and expression of Bcl-2 was up-regulated(P<0.05

P<0.01). Conclusion:SXTAR can protect HUVECs injured by ox-LDL

which may be related with inhibiting the endothelial cell apoptosis

reducing inflammation and resisting oxidation.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Hei Xiaoyaosan Modulates Oxidative Stress and Apoptosis to Exert Neuroprotective Effect in Alzheimer's Disease Rats

Effect of Linggui Zhugantang on Mitochondrial Fusion-fission and Sirt3/ AMPK Signaling Pathway in Chronic Heart Failure Rats After Myocardial Infarction

Protective Effect of Shentong Zhuyutang-containing Serum on Chondrocytes in Rats with Knee Osteoarthritis Based on AMPK/Sirt1 Signaling Pathway

Role of p38 MAPK Pathway in Diabetic Peripheral Neuropathy and Chinese Medicine Intervention： A Review

Effect of Ferroptosis on Atherosclerosis and Chinese Medicine Intervention： A Review

Related Author

CHEN Yiqin

YANG Jiao

PEI Wenli

HAN Yumei

WANG Huping

YAO Juan

DING Rui

LI Xiangyang

Related Institution

Gansu University of Chinese Medicine

Gansu Key Laboratory of Traditional Chinese Medicine（TCM）Excavation and Innovative Transformation

Gansu Engineering Laboratory for New Products of TCM

School of Integrated Chinese Medicine and Western Medicine，Anhui University of Chinese Medicine

School of Chinese Medicine，Anhui University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰