Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four <italic style="font-style: italic">Euphorbia</italic> Factors Based on Thinking of Self-assembled Micelles <italic style="font-style: italic">in vivo</italic>

Tong SU; Rui YUAN; Chao ZHANG; Hui-hui GAO; Ying-zi WANG; Xiu-mei SUN

doi:10.13422/j.cnki.syfjx.20182302

您当前的位置：

首页 >

文章列表页 >

Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four Euphorbia Factors Based on Thinking of Self-assembled Micelles in vivo

Pharmacy and Processing | 更新时间：2021-02-09

- Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four Euphorbia Factors Based on Thinking of Self-assembled Micelles in vivo
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 160-164(2019)
- 作者机构：
  
  1.山东中医药大学　药学院，济南　 250355；
  2.北京中医药大学，北京　 100102
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182302
  CLC： R22;R94;R28;O657.7
- Received：04 May 2018，
  
  Published Online：12 September 2018，
  
  Published：05 April 2019
- 稿件说明：
移动端阅览
Tong SU, Rui YUAN, Chao ZHANG, et al. Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four Euphorbia Factors Based on Thinking of Self-assembled Micelles in vivo[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 160-164.
DOI：

Tong SU, Rui YUAN, Chao ZHANG, et al. Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four Euphorbia Factors Based on Thinking of Self-assembled Micelles in vivo[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 160-164. DOI： 10.13422/j.cnki.syfjx.20182302.

摘要

目的：

通过模拟人体肠道环境，研究千金子脂肪油与肠液中胆盐所形成混合胶束对千金子素L

，L

的增溶作用。

方法：

制备不同千金子脂肪油加入量的混合胶束，采用透射电子显微镜观察胶束的形态，运用粒度仪测定其粒径和Zeta电位，利用HPLC考察混合胶束对4种千金子素溶解度的影响，同时考察放置时间对混合胶束稳定性的影响。

结果：

体外模拟形成的千金子脂肪油/胆盐混合胶束呈球形且粒径均一，Zeta电位绝对值均

20 mV；固定脱氧胆酸钠质量浓度为4.96 g·L

－1

，4种千金子素在千金子脂肪油质量浓度为0.1～4 g·L

－1

时的溶解度均显著大于不含千金子脂肪油时的溶解度，混合胶束中4种千金子素的溶解度为其在不含脂肪油胶束体系溶解度的1.3～4倍；该混合胶束可稳定存在36 h。

结论：

千金子脂肪油与脱氧胆酸钠所形成的混合胶束对千金子素L

，L

有显著的增溶作用，可为从药剂学角度阐释千金子去油制霜的减毒机制提供参考。

Abstract

Objective:

The solubilization of 4 compounds(

Euphorbia

factor L

and L

)

caused by mixed micelles self-assembled from fatty oil of Euphorbiae Semen and bile salt of intestinal juice

was researched in the simulated human intestinal environment.

Method:

The mixed micelles were prepared with different amounts of fatty oil of Euphorbiae Semen.The transmission electron microscope(TEM) was used to observe the morphology of the micelles.Particle size detector was used to determine the particle size and Zeta potential.HPLC was used to assay the solubility of these 4 compounds.The variation tendency of the total dissolution of these 4 compounds with the change of standing time was observed.

Result:

Particle size of the mixed micelles was uniform and its morphology was spherical.The absolute values of Zeta potential were less than 20 mV.When the amount of sodium deoxycholate was fixed 4.96 g·L

-1

the solubility of these 4 compounds with the concentration of fatty oil at 0.1-4 g·L

-1

were significantly greater than that at the dosage of 0 g·L

-1

.The solubility of these 4 compounds in the micelles formed by fatty oil was 1.3 to 4 times as much as the micelles without fatty oil.The micelles was stable for 36 h.

Conclusion:

The micelles self-assembled from fatty oil of Euphorbiae Semen and bile salt of intestinal juice

have significant solubilization effect on

Euphorbia

factor L

and L

.This research can lay the foundation for clarifying the detoxification mechanism of removing fatty oil and making frostlike powder from the perspective of pharmaceutics.

关键词

Keywords

references

　窦志华，丁安伟．大戟属有毒中药毒性成分及炮制减毒研究进展 [C]// 中华中医药学会．第六届中药炮制学术会议论文集：2006年卷．北京：中国学术期刊（光盘版）电子杂志社， 2006 ： 226 - 232 .

刘玉婷，杨洋，弓佩含，等．千金子化学成分研究进展 [J]. 中国实验方剂学杂志， 2017 , 23 ( 13 ): 220 - 225 .

ZHANG J Y , ZHANG C , CHEN H B , et al . Assignments of 1 H and 13 C-NMR signals of Euphorbia factor L 1 and investigation of its anticancer activity in vitro [J]. J Med Plants Res , 2010 , 4 ( 4 ): 335 - 338 .

　郑飞龙．千金子毒性成分的研究 [D]．南昌：南昌大学， 2009 .

张宏伟，金锋，张振凌．泛油对千金子主要有效成分含量的影响 [J]. 中国实验方剂学杂志， 2012 , 18 ( 22 ): 124 - 126 .

孙付军，宋卫国，李英霞．千金子及不同含油量千金子霜急性毒性比较 [J]. 中国药物警戒， 2011 , 8 ( 1 ): 20 - 22 .

张超，朱娟娟，王英姿，等． HPLC法测定千金子不同极性部位中二萜酯类化合物含量 [J]. 山东中医药大学学报， 2014 , 38 ( 5 ): 485 - 488 .

Yeap Y Y , Trevaskis N L , Porter C J. Lipid absorption triggers drug supersaturation at the intestinal unstirred water layer and promotes drug absorption from mixed micelles [J]. Pharm Res , 2013 , 30 ( 12 ): 3045 - 3058 .

姚静，周建平，杨宇欣，等．微乳对难溶性药物增溶机理的研究 [J]. 中国药科大学学报， 2004 , 35 ( 6 ): 495 - 498 .

Hammad M A , Müller B W. Increasing drug solubility by means of bile salt-phosphatidylcholine-based mixed micelles [J]. Eur J Pharm Biopharm , 1998 , 46 ( 3 ): 361 - 367 .

朱娟娟，王英姿，张超，等．千金子石油醚萃取物中油脂成分的GC-MS分析 [J]. 山东中医药大学学报， 2013 , 37 ( 5 ): 439 - 441 .

李杰，孙娥，张振海，等．羊脂油对淫羊藿活性黄酮自组装胶束模拟形成的影响 [J]. 中国中药杂志， 2014 , 39 ( 17 ): 3278 - 3282 .

张琼，李林，魏东芝，等．胆酸盐/脂类混合胶束对疏水性姜黄素的增溶性能 [J]. 华东理工大学学报：自然科学版， 2010 , 36 ( 5 ): 639 - 644 .

Khurana R K , Beg S , Burrow A J , et al . Enhancing biopharmaceutical performance of an anticancer drug by long chain PUFA based self-nanoemulsifying lipidic nanomicellar systems [J]. Eur J Pharm Biopharm , 2017 , doi: 10.1016/j.ejpb.2017.09.001 doi:10.1016/j.ejpb.2017.09.001 .

van Hasselt P M , Janssens G E , Slot T K , et al . The influence of bile acids on the oral bioavailability of vitamin K encapsulated in polymeric micelles [J]. J Controlled Release , 2009 , 133 ( 2 ): 161 - 168 .

张秀婷，王英姿，李韶菁，等．在体单向肠灌流模型研究千金子甾醇在大鼠肠吸收特性 [J]. 北京中医药大学学报， 2015 , 38 ( 9 ): 624 - 627 .

MENG X , ZHAO X , LONG Z , et al . A sensitive liquid chromatography-mass spectrometry method for simultaneous determination of three diterpenoid esters from Euphorbia lathyris L. in rat plasma [J]. J Pharm Biomed Anal , 2013 , 72 : 299 - 305 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of Rancidity Mechanism of Armeniacae Semen Amarum During Storage

Extraction Process of Fatty Oil from Fructus Leonuri by Supercritical Fluid

Advance in Study of Polymer Micelle Drug Carrier Systems

Study on Stability and Solubilization of Oil Hydroxypropyl--cyclodextrin Inclusion Complex

Determination of 3 Kinds of Fatty Acids in Arctium Fructus by GC

Related Author

Jin-qian YU

Kai WANG

Lan-ping GUO

Xiao WANG

Yun-xia CHENG

TAO Gui-bin

LI Yin-qing

GAO Wen-yi

Related Institution

School of Pharmacy，Shandong University of Traditional Chinese Medicine

Qilu University of Technology （Shandong Academy of Sciences）， Shandong Analysis and Test Center

State Key Laboratory Breeding Base of Dao-di Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences

Department of Pharmacy,Zhujiang Hospital,Southern Medical University

Changchun University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Analysis of Solubilization of Fatty Oil from Euphorbiae Semen on Four Euphorbia Factors Based on Thinking of Self-assembled Micelles in vivo

DOI：10.13422/j.cnki.syfjx.20182302

摘要

Abstract

关键词

Keywords

references