Analysis on Changes of Chemical Components in Enzymolysis Process of Raphani Semen Pieces Based on HPLC-DAD Characteristic Spectra

Li-qiao ZHU; Shao-hua YU; Xi ZHANG; Hong-lei ZHOU; Hua-gang SHENG

doi:10.13422/j.cnki.syfjx.20182304

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Analysis on Changes of Chemical Components in Enzymolysis Process of Raphani Semen Pieces Based on HPLC-DAD Characteristic Spectra

Pharmacy and Processing | 更新时间：2021-02-09

- Analysis on Changes of Chemical Components in Enzymolysis Process of Raphani Semen Pieces Based on HPLC-DAD Characteristic Spectra
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 140-145(2019)
- 作者机构：
  
  山东中医药大学　药学院，济南　 250355
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182304
  CLC： R22;O657.7;R28;R943.1
- Received：27 May 2018，
  
  Published Online：12 September 2018，
  
  Published：20 February 2019
- 稿件说明：
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Li-qiao ZHU, Shao-hua YU, Xi ZHANG, et al. Analysis on Changes of Chemical Components in Enzymolysis Process of Raphani Semen Pieces Based on HPLC-DAD Characteristic Spectra[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 140-145.
DOI：

Li-qiao ZHU, Shao-hua YU, Xi ZHANG, et al. Analysis on Changes of Chemical Components in Enzymolysis Process of Raphani Semen Pieces Based on HPLC-DAD Characteristic Spectra[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 140-145. DOI： 10.13422/j.cnki.syfjx.20182304.

摘要

目的：

分析莱菔子饮片酶解过程中化学成分的动态变化。

方法：

采用HPLC分析莱菔子不同酶解时间的特征图谱，色谱条件为流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0～15 min，5%～10%A；15～17 min，10%～12.5%A；17～27 min，12.5%～14%A；27～55 min，14%～25%A；55～57 min，25%～70%A；57～70 min，70%～100%A)，检测波长225 nm。标定其特征峰，同时提取各特征峰的UV光谱，比较不同特征峰UV光谱的异同和HPLC特征图谱特征峰峰面积的变化，分析莱菔子中特征成分的动态变化规律。

结果：

莱菔子不同酶解时间的特征图谱中共标定11个特征峰，归属了萝卜苷和芥子碱硫氰酸盐2个色谱峰，同时发现莱菔子中萝卜苷会快速被黑芥子酶酶解，先被酶解为一中间体，继而被分解为其他成分。芥子碱硫氰酸盐随着酶解时间没发生明显的变化。芥子酸苷类成分也会被酶解。

结论：

莱菔子的酶解不单是萝卜苷的酶解，还包括芥子酸苷类成分的酶解，可为莱菔子“生熟异治，生升熟降”的药性变化提供参考。

Abstract

Objective:

To analyze the dynamic changes of components in the enzymolysis process of raw products of Raphani Semen.

Method:

HPLC was employed to analysis of characteristic spectra of Raphani Semen at different enzymolysis time with mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution and detection wavelength at 225 nm.The characteristic peaks were calibrated

meanwhile

the UV spectra of characterstic peaks were extracted

and the difference between UV spectra and the changes of peak areas were compared

and the dynamic changes of characterstic components in Raphani Semen were analyzed.

Result:

Eleven characteristic peaks were marked from the characteric spectra of raw products of Raphani Semen at different enzymolysis time

and glucoraphenin and sinapine thiocyanate were assigned.Glucoraphenin was enzymatically hydrolyzed fastly by myrosinase

and an intermediate was generated

and then continue to be decomposed into other components.Sinapine thiocyanate did not change significantly during the enzymolysis process

and sinadiosides was also enzymatically degraded.

Conclusion:

The enzymolysis of Raphani Semen is not only the glucoraphenin

but also the sinadiosides.This paper can provide reference for the property change of Raphani Semen in processing.

关键词

Keywords

references

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