UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration

Xiao SHEN; Hua-zhu ZHENG; Ying-jiao MENG; Xiang-li YAN; Sheng-xin WANG; Ai-ming YU; Li-sheng WANG

doi:10.13422/j.cnki.syfjx.20182309

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UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration

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- UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 2, Pages: 8-13(2019)
- 作者机构：
  
  广州中医药大学　中药学院，广州　 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182309
  CLC： R22; R945; R28; O657.6
- Received：25 February 2018，
  
  Published Online：12 September 2018，
  
  Published：20 January 2019
- 稿件说明：
移动端阅览
Xiao SHEN, Hua-zhu ZHENG, Ying-jiao MENG, et al. UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 8-13.
DOI：

Xiao SHEN, Hua-zhu ZHENG, Ying-jiao MENG, et al. UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 8-13. DOI： 10.13422/j.cnki.syfjx.20182309.

摘要

目的：

对大鼠灌胃补阳还五汤提取物后的吸收入脑及入血成分进行分析，为该复方的临床应用提供参考。

方法：

采用UPLC-Q-TOF-MS/MS对正常大鼠脑组织、血浆和脑缺血再灌注大鼠脑组织、血浆进行分析，色谱条件为流动相甲醇-0.1%甲酸水溶液梯度洗脱，流速0.3 mL·min

－1

，进样量3 μL；质谱条件为电喷雾离子源，正、负离子模式扫描，扫描范围

50～1 000。根据保留时间、精确相对分子质量、一级和二级质谱数据等鉴定补阳五汤中的原型成分。

结果：

补阳还五汤提取物给药后，发现5个化合物能透过血脑屏障进入正常脑组织，鉴定为毛蕊异黄酮苷、芍药内酯苷、芒柄花素-7-

-葡萄糖苷-6″-

-乙酰基、红花黄色素C和黄芪甲苷；2个化合物能透过血脑屏障进入造模脑组织，鉴定为毛蕊异黄酮苷和芒柄花素-7-

-葡萄糖苷-6″-

-乙酰基；7个化合物进入正常血浆中，经鉴定为毛蕊异黄酮苷、芍药内酯苷、羟基红花黄色素A等；3个化合物进入造模血浆中，鉴定为毛蕊异黄酮-7-

-葡萄糖苷-6″-

-乙酰基，6″-

-乙酰基-(6

αR

，11

αR

)-9，10-二甲氧基紫檀烷-3-

-葡糖苷和芒柄花素-7-

-葡萄糖苷-6″-

-乙酰基。

结论：

补阳还五汤在正常与脑缺血损伤大鼠中的药效物质基础不尽相同，可为该复方的作用机制研究奠定基础。

Abstract

Objective:

To analyze and identify the brain and blood absorption components of rats after intragastric administration of Buyang Huanwu Tang(BYHWT).

Method:

The brain tissue

plasma of normal rats and the cerebral ischemia-reperfusion rats were analyzed by UPLC-Q-TOF-MS/MS.The prototype components in BYHWT were identified according to retention time

accurate relative molecular weight

primary and secondary mass spectrometry data.

Result:

After the administration of BYHWT

five compounds were found to enter the normal brain tissue through the blood-brain barrier and identified as calycosin-7-glucoside

albiflorin

formononetin-7-

-glucoside-6″-

-acetyl

safflower yellow A and astragaloside A; two compounds penetrated the blood-brain barrier and entered modeling brain tissue

and they were identified as calycosin-7-glucoside and formononetin-7-

-glucoside-6″-

-acetyl; seven compounds entered normal plasma and were identified as calycosin-7-glucoside

albiflorin

hydroxysafflor yellow A

et al; three compounds entered model plasma and identified as calycosin-7-

-glucoside-6″-

-acetyl

6″-

-acetyl-(6

αR

)-9

10-dimetho-xypterocarpan-3-

-glucoside and formononetin-7-

-glucoside-6″-

-acetyl.

Conclusion:

BYHWT has different pharmacological material basis in normal and cerebral ischemia-reperfusion rats.

关键词

Keywords

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