Effect of Polysaccharides from Eucommiae Cortex on Expressions of Genes of Ⅰ, Ⅲ Collagen, MMP-1, TIMP-1 and TGF- from Hepatic Fibrosis in Rat Models

WANG Qian-yu; WANG Wen-jia; XI Jing; CAI Kun; Wang Ping; LIANG Jian-dong; HAN Jie; HE Guang-zhi

doi:10.13422/j.cnki.syfjx.20182334

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Effect of Polysaccharides from Eucommiae Cortex on Expressions of Genes of Ⅰ, Ⅲ Collagen, MMP-1, TIMP-1 and TGF- from Hepatic Fibrosis in Rat Models

更新时间：2024-09-23

- Effect of Polysaccharides from Eucommiae Cortex on Expressions of Genes of Ⅰ, Ⅲ Collagen, MMP-1, TIMP-1 and TGF- from Hepatic Fibrosis in Rat Models
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 23, Pages: 153-158(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182334
  CLC： R-332;R285.5
- Published：2018
- 稿件说明：
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WANG Qian-yu, WANG Wen-jia, XI Jing, et al. Effect of Polysaccharides from Eucommiae Cortex on Expressions of Genes of Ⅰ, Ⅲ Collagen, MMP-1, TIMP-1 and TGF- from Hepatic Fibrosis in Rat Models[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 153-158.
DOI：

WANG Qian-yu, WANG Wen-jia, XI Jing, et al. Effect of Polysaccharides from Eucommiae Cortex on Expressions of Genes of Ⅰ, Ⅲ Collagen, MMP-1, TIMP-1 and TGF- from Hepatic Fibrosis in Rat Models[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 153-158. DOI： 10.13422/j.cnki.syfjx.20182334.

摘要

目的：探讨杜仲多糖治疗肝纤维化（HF）作用，并探讨其相关的作用机制。方法：将60只健康SD大鼠随机分成2组，正常组（10只）和肝纤维化造模组（50只）。造模组采用40%四氯化碳（CCl4）腹腔注射制备HF动物模型，造模成功后将其随机分为5个组，分别为模型组，杜仲多糖高、中、低剂量组及秋水仙碱组每组10只。秋水仙碱组（1.0×10-4g·kg-1），杜仲多糖高、中、低剂量组（0.14，0.07，0.035 g·kg-1）分别连续灌胃给药8周后收集样本，测定法大鼠体质量及计算肝脏系数；采用酶联免疫吸附测定（ELISA）检测血清白细胞介素-6（IL-6），高迁移率族蛋白B1（high-mobility group box 1，HMGB1），脂多糖（LPS），肝组织基质金属蛋白酶-1（MMP-1）和金属蛋白酶组织抑制因子-1（TIMP-1）水平；逆转录聚合酶链式反应（RT-PCR）技术分析各组大鼠肝组织Ⅰ，Ⅲ型胶原蛋白，MMP-1，TIMP-1及转化生长因子-β1（TGF-β1）mRNA表达情况。结果：与正常组比较，CCl4能显著降低实验大鼠体质量（P<0.01），提高肝脏系数（P<0.01）；杜仲多糖能显著增加HF模型的体质量（P<0.01），显著降低HF模型肝脏系数（P<0.01）；ELISA检测表明，杜仲多糖能显著降低肝纤维化大鼠血清IL-6，HMGB1及LPS含量（P<0.01）；RT-PCR检测结果显示，杜仲多糖及秋水仙碱能显著降低HF肝脏中Ⅰ，Ⅲ型胶原蛋白，TIMP-1及TGF-β1 mRNA含量（P<0.05），明显提高MMP-1含量（P<0.05，P<0.01），且呈量效关系。结论：杜仲多糖具有显著的抗肝纤维化作用，其作用机制可能与抑制降低HF肝脏中Ⅰ，Ⅲ型胶原蛋白，TIMP-1及TGF-β1 mRNA表达有关。

Abstract

Objective:To investigate the therapeutic effect of polysaccharides from Eucommiae Cortex on liver fibrosis

in order to provide the experimental basis for the development of new anti-liver fibrosis drugs. Method:The 60 healthy SD rats were randomly divided into 2 groups

namely the normal control group (n=10) and the hepatic fibrosis(HF) group (n=50). The model of liver fibrosis was prepared through intraperitoneal injection with 40%CCl4 in the model group. The model was randomly divided into 5 groups:the model control group

the high

middle and low-dose polysaccharides from Eucommiae Cortex groups and the colchicine group

with 10 rats in each group. After successful modeling

colchicine group (1×10-4g·kg-1)

and high

medium and low-dose polysaccharides from Eucommiae Cortex groups (0.14

0.07

0.035 g·kg-1) were given drugs by gavage for 8 weeks

and then samples were collected to determine the mass of rats and calculate the liver coefficient. The levels of serum interleukin-6 (IL-6)

high-mobility group box 1 (HMGB1)

lipopolysaccharide (LPS) and the levels of matrix metalloproteinse-1(MMP-1) and tissue inhibitors of metalloproteinase-1(TIMP-1) in liver tissues were detected by enzyme-linked immunosorbent assay(ELISA). The expressions of Ⅰ

Ⅲ type collagen

MMP-1

TIMP-1 and transforming growth factor-β1(TGF-β1) in liver tissues of rats were analyzed by reverse transcription-polymerase chain reaction(RT-PCR) technique. Result:CCl4 can significantly reduce the body mass (P<0.01) of experimental rats and increase the liver coefficient (P<0.01). Polysaccharides from Eucommiae Cortex can significantly increase the body mass of the HF model (P<0.01)

and significantly lower the HF model liver coefficient (P<0.01). ELISA detection showed that polysaccharides from Eucommiae Cortex can significantly reduce IL-6

HMGB1 and LPS content (P<0.01) in the serum of liver fibroid rats. Polysaccharides from Eucommiae Cortex and colchicine could significantly reduce the mRNA content of Ⅰ

Ⅲ type collagen

TIMP-1 and TGF-β1 (P<0.05

P<0.01) in rat liver

and significantly increase the content of MMP-1 (P<0.05

P<0.01)

with a dose-effect relationship. Conclusion:Polysaccharides from Eucommiae Cortex had a significant anti-hepatic fibrosis effect. The mechanism may be related to the inhibition of the expressions of I

type Ⅲ collagen

TIMP-1 and TGF-β1 in HF liver.

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