Effect of Different Antisepsis Measures on Endogenous Metabolites in Rat Urine

Huan LIU; Jie GAO; Jing-jing YIN; Li-qun HUANG; Wen-li XU; Hui YUAN; Juan YUE; Xiu-jun QIN; Jian-guo LI; Jun-sheng TIAN

doi:10.13422/j.cnki.syfjx.20182402

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Effect of Different Antisepsis Measures on Endogenous Metabolites in Rat Urine

Drug Metabolism | 更新时间：2021-02-09

- Effect of Different Antisepsis Measures on Endogenous Metabolites in Rat Urine
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 6, Pages: 120-124(2019)
- 作者机构：
  
  1.中国辐射防护研究院　药物安全性评价中心，药物毒理与放射损伤药物山西省重点实验室，中核放射毒理与放射性药物临床前评价重点实验室，太原　 030006
  2.山西大学　中医药现代研究中心，地产中药功效物质研究与利用山西省重点实验室，太原　 030006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182402
  CLC： R22;R969.1;R28;C37;R446.12
- Received：23 May 2018，
  
  Published Online：2018-27-09，
  
  Published：20 March 2019
- 稿件说明：
移动端阅览
Huan LIU, Jie GAO, Jing-jing YIN, et al. Effect of Different Antisepsis Measures on Endogenous Metabolites in Rat Urine[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 120-124.
DOI：

Huan LIU, Jie GAO, Jing-jing YIN, et al. Effect of Different Antisepsis Measures on Endogenous Metabolites in Rat Urine[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 120-124. DOI： 10.13422/j.cnki.syfjx.20182402.

摘要

目的：

在大鼠尿液代谢组学研究中需要对尿液样本的采集过程采取必要的防腐措施，针对尿液收集过程中常用的几种防腐措施，研究其对尿液内源性代谢物的影响。

方法：

在大鼠尿液样本采集时采用冰上收集、加入叠氮化钠以及两者合用进行防腐，利用核磁共振代谢组学技术分析不同防腐措施的差异。

结果：

核磁共振代谢组学结果显示，冰组和叠氮化钠＋冰合用组的主成分分析结果重合较多，且二者与空白组、叠氮化钠组均有较高的分离度；叠氮化钠组和空白组有小部分重叠，但是有分离的趋势。提示冰组与叠氮化钠＋冰合用组的防腐效果相近，与空白组相比尿液中的缬氨酸、甜菜碱、马尿酸上升，丙氨酸和2-酮戊二酸下降。

结论：

在大鼠尿液代谢组学研究中，尿液样本采集时一定要有低温防腐措施，在有防护条件时加入一定量叠氮化钠有助于提高防腐效果。

Abstract

Objective:

In the study of urine metabolomics of rats

necessary antiseptic measures should be taken for collection of urine samples

the effect of several antiseptic measures on the endogenous metabolites in urine was studied.

Method:

The urine samples of rats were collected on ice

sodium azide was added

and both of them were used together to prevent corrosion. Differences of antiseptic measures were analyzed by nuclear magnetic resonance (NMR) metabolomics.

Result:

The results of NMR metabolomics showed that sodium azide+ ice group and ice group had many overlaps

but they clearly separated with the control group and sodium azide group; sodium azide group and the control group had a small part overlap

but there was a tendency of separation. The antiseptic effect of sodium azide+ ice group and ice group was similar; compared with control group

valine

betaine and hippuricacid in these two groups increased

but the alanine and 2-ketoglutaric acid decreased.

Conclusion:

In the study of rat urine metabolomics

low temperature antiseptic measures must be taken when urine samples are collected

and the addition of sodium azide can improve the antiseptic effect slightly under protective conditions.

关键词

Keywords

references

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