Discussion on Theory of "Wine Processing Could Promote Efficacy" Based on Brain Protective Effect of Raw and Wine-processed Products of Rhei Radix et Rhizoma on ICH Rats

Kun WANG; Chang-jiang HU; Xu ZHAO; Ying-lin CUI

doi:10.13422/j.cnki.syfjx.20182405

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Discussion on Theory of "Wine Processing Could Promote Efficacy" Based on Brain Protective Effect of Raw and Wine-processed Products of Rhei Radix et Rhizoma on ICH Rats

Pharmacy and Processing | 更新时间：2021-02-09

- Discussion on Theory of "Wine Processing Could Promote Efficacy" Based on Brain Protective Effect of Raw and Wine-processed Products of Rhei Radix et Rhizoma on ICH Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 6, Pages: 130-135(2019)
- 作者机构：
  
  1.成都中医药大学，成都　 611137；
  2.河南省中医院，郑州　 450002
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182405
  CLC： R22;R285;R283;R943.1;R96
- Received：03 June 2018，
  
  Published Online：2018-27-09，
  
  Published：20 March 2019
- 稿件说明：
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Kun WANG, Chang-jiang HU, Xu ZHAO, et al. Discussion on Theory of "Wine Processing Could Promote Efficacy" Based on Brain Protective Effect of Raw and Wine-processed Products of Rhei Radix et Rhizoma on ICH Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 130-135.
DOI：

Kun WANG, Chang-jiang HU, Xu ZHAO, et al. Discussion on Theory of "Wine Processing Could Promote Efficacy" Based on Brain Protective Effect of Raw and Wine-processed Products of Rhei Radix et Rhizoma on ICH Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(6): 130-135. DOI： 10.13422/j.cnki.syfjx.20182405.

摘要

目的：

研究生大黄、酒大黄提取液对脑出血(ICH)模型大鼠脑保护作用的差异，探讨大黄“酒制升提”理论的内涵。

方法：

Wistar雄性大鼠建立ICH模型，用生大黄、酒大黄提取液进行干预，并随机分成6组（空白组、假手术组、模型组、生大黄组、酒大黄组、安宫牛黄丸组）；脱氧核糖核苷酸末端转移酶介导的缺口末端标记（TUNEL）检测海马组织神经细胞凋亡的情况，免疫组化法检测出血侧脑组织紧密连接附着蛋白-1（ZO-1）和血管内皮钙黏蛋白-2（VE-cadherin-2）蛋白表达情况，蛋白免疫印迹试验（Western blot）检测出血侧脑组织还原型辅酶Ⅱ（NADPH）氧化酶2（NOX2）和肿瘤坏死因子-

刺激基因6（TSG-6）蛋白的表达，全自动酶标仪检测ICH大鼠血清中还原型谷胱甘肽（GSH）含量；研究生大黄、酒大黄对ICH大鼠脑保护作用的差异。

结果：

与假手术组比较，模型组大鼠海马组织神经细胞凋亡指数显著增加(

0.01)，ZO-1表达量显著下降(

0.01)，NOX2蛋白表达量显著升高(

0.01)，TSG-6蛋白表达量显著下降(

0.01)，GSH的含量显著下降(

0.01)；同模型组比较，酒大黄、生大黄均能显著降低ICH大鼠海马组织的神经细胞凋亡指数(

0.01)，均显著升高ZO-1蛋白表达(

0.01)，升高VE-cadherin-2蛋白表达(

0.05)，显著降低NOX2蛋白的表达(

0.01，

0.05)，显著升高TSG-6蛋白的表达(

0.05)，显著升高GSH含量(

0.01)。

结论：

生大黄、酒大黄对ICH大鼠模型均具有脑保护作用，但酒大黄的脑保护作用略优于生大黄，为探讨大黄“酒制升提”理论提供了实验依据。

Abstract

Objective:

To explore the difference of effect of raw and wine-processed products of Rhei Radix et Rhizoma extract on brain protection of rats with intracerebral hemorrhage(ICH) and the connotation of the theory of "wine processing could promote efficacy" of Rhei Radix et Rhizoma.

Method:

Wistar male rats were used to establish the ICH model

extracts of raw and wine-processed products of Rhei Radix et Rhizoma were used for intervention

and these rats were randomly divided into 6 groups(blank group

sham-operated group

model group

raw Rhei Radix et Rhizoma group

wine-processed Rhei Radix et Rhizoma group and Angong Niuhuangwan group). Then the apoptosis of hippocampal neurons was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay. The expression of zonula occludens-1(ZO-1) and vascular endothelial cadherin-2(VE-cadherin-2) in the hemorrhagic brain tissue was detected by immunohistochemical assay. The expression of reduced form of nicotinamide-adenine dinucleotide phosphate(NADPH) oxidase 2(NOX2) and tumor necrosis factor-

stimulated gene-6(TSG-6) in the hemorrhagic brain tissue was detected by Western blot. The content of glutathione(GSH) in the serum of ICH rats was detected by automatic enzyme-linked immunosorbent assay systems. Through these above methods

we investigated the differences between raw products and wine-processed products on brain protection of ICH rats.

Result:

Compared with the sham-operated group

the apoptosis index of the hippocampal neurons in the model group were increased significantly(

0.01)

the expression of ZO-1 was decreased significantly(

0.01)

the expression of NOX2 protein was increased significantly(

0.01)

the expression of TSG-6 protein was decreased significantly(

0.01)

the content of GSH was also decreased significantly(

0.01). Compared with the model group

raw and wine-processed products of Rhei Radix et Rhizoma could reduce the apoptosis index of hippocampal neurons(

0.01)

increase the expression of ZO-1(

0.01) and VE-cadherin-2 protein(

0.05)

decrease the expression of NOX2(

0.05

0.01)

increase the expression of TSG-6 protein(

0.05) and increase the content of GSH(

0.01) in ICH rats.

Conclusion:

The raw and wine-processed products of Rhei Radix et Rhizoma all have brain protective effect on ICH rat model

but the protective effect of wine-processed products is slightly better than that of raw products. The result of this study provides experimental basis for exploring the theory of "wine processing could promote efficacy" of this herb.

关键词

Keywords

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