Isolation and Identification of Main Flavonoid Glycosides of <italic style="font-style: italic">Dendrobium officinale</italic> from Danxia Species and Yunnan Guangnan Species

Xiao-feng ZHANG; Chun-hua ZHOU; Long-kai ZHANG; Mei JIANG; Zhen-shan XIE; Yuan YUAN; Yue-chun HUANG; Ying-yi LUO; Gang WEI

doi:10.13422/j.cnki.syfjx.20182417

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Isolation and Identification of Main Flavonoid Glycosides of Dendrobium officinale from Danxia Species and Yunnan Guangnan Species

Topic on Germplasm Resources of Dendrobii Officinalis Caulis | 更新时间：2021-02-09

- Isolation and Identification of Main Flavonoid Glycosides of Dendrobium officinale from Danxia Species and Yunnan Guangnan Species
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 29-34(2019)
- 作者机构：
  
  1.广州中医药大学　中药学院，广州　 510006
  2.广州中医药大学　第一附属医院，广州　 510405
  3.中国中医科学院　中药资源中心，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182417
  CLC： R284.1;R282.6;R289;R22
- Received：22 June 2018，
  
  Published Online：28 September 2018，
  
  Published：05 January 2019
- 稿件说明：
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Xiao-feng ZHANG, Chun-hua ZHOU, Long-kai ZHANG, et al. Isolation and Identification of Main Flavonoid Glycosides of Dendrobium officinale from Danxia Species and Yunnan Guangnan Species[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 29-34.
DOI：

Xiao-feng ZHANG, Chun-hua ZHOU, Long-kai ZHANG, et al. Isolation and Identification of Main Flavonoid Glycosides of Dendrobium officinale from Danxia Species and Yunnan Guangnan Species[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 29-34. DOI： 10.13422/j.cnki.syfjx.20182417.

摘要

目的：

对2个道地种源（丹霞种、云南广南种）铁皮石斛中的黄酮苷成分进行提取分离、纯化及结构鉴定，为确认铁皮石斛的共性黄酮苷成分提供实验依据。

方法：

①采用70%乙醇提取两个产地铁皮石斛叶的总黄酮成分，依次采用有机溶剂石油醚、乙酸乙酯、水饱和正丁醇萃取粗提物，运用大孔吸附树脂AB-8，LH-20型羟丙基葡聚糖凝胶（Sephadex LH-20）以及ODS柱色谱对水饱和正丁醇萃取部位进行分离纯化，通过化合物的理化性质结合HPLC

ESI-MS

，

H-NMR，

C-NMR等现代波谱学方法鉴定黄酮苷结构。②利用HPLC分析比较不同道地种源（丹霞种、云南广南种、广西铁皮兰种、浙江本地种）铁皮石斛的黄酮苷共有成分。

结果：

从2个产地铁皮石斛叶中共分离得到了5个黄酮苷化合物，经鉴定为芦丁，新西兰牡荆苷Ⅱ，新西兰牡荆苷Ⅰ，佛莱心苷，异佛莱心苷。4个不同道地种源（丹霞种、云南广南种、广西铁皮兰种、浙江本地种）铁皮石斛茎中均能检出新西兰牡荆苷Ⅱ，新西兰牡荆苷Ⅰ特征峰，其中新西兰牡荆苷Ⅱ丰度相对较大，而佛莱心苷、异佛莱心苷在云南广南种、广西铁皮兰种中出峰明显，芦丁则在丹霞种中尤为突出。

结论：

新西兰牡荆苷Ⅱ为铁皮石斛不同道地种源（丹霞种、云南广南种、广西铁皮兰种、浙江本地种）的共性黄酮苷成分，可作为铁皮石斛特征图谱的参照物。

Abstract

Objective:

To extract

isolate

purify and identify the structures of the flavonoid glycoside in

Dendrobium officinale

from two different origin places (Danxia species and Yunnan Guangnan species)

and provide experimental reference for confirming the common flavonoid glycoside components in

officinale

Method:

①70% ethanol was applied to extract the total flavonoids in leaves of

officinale

from two different species. Organic solvents petroleum ether

acetic ether and water saturated

-butyl alcohol were used in turn to extract the crude extraction. Then AB-8 Macroporous resin

Sephadex LH-20 and ODS chromatographic column were applied to isolate and purify the water saturated

-butyl alcohol extraction fraction. The structures of flavonoid glycoside were identified by studying physicochemical property

applying modern spectroscopy method like HPLC

ESI-MS

H-NMR

C-NMR

etc. ②HPLC characteristic spectrum technique was used to analyse and compare the common flavonoid glycoside components in

Dendrobium officinale

from different origin places (Danxia species

Yunnan Guangnan species

Guangxi Tiepilan species and Zhejiang native species).

Result:

Five flavonoid glycoside compounds were isolated from the crude extractions of the leaves of

officinale

from two different species

and they were identified as rutin

vicenin Ⅱ

viceninⅠ

violanthin and isoviolanthin. The characteristic spectrum of vicenin Ⅱ and viceninⅠwere detected in stems of

officinale

from four different origin places (Danxia species

Yunnan Guangnan species

Guangxi Tiepilan species and Zhejiang native species)

and vicenin Ⅱ had a better separation degree in the characteristic spectrum. However

the characteristic spectrum of violanthin and isoviolanthin were more obvious in Yunnan Guangnan species and Guangxi Tiepilan species

while rutin was obvious in the Danxia species.

Conclusion:

Vicenin Ⅱis the common flavonoid glycosides component in

officinale

from different origin places (Danxia species

Yunnan Guangnan species

Guangxi Tiepilan species and Zhejiang native species)

and can be used as the internal reference material for the characteristic spectrum of

officinale

关键词

Keywords

references

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Related Author

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Yun-rong LI

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Related Institution

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Isolation and Identification of Main Flavonoid Glycosides of Dendrobium officinale from Danxia Species and Yunnan Guangnan Species

DOI：10.13422/j.cnki.syfjx.20182417

摘要

Abstract

关键词

Keywords

references