Objective: To assess the activity and action modes of Qingre Jiedu prescription in resisting human papillomavirus 16 (HPV16) in vitro by establishing the cell model infected by HPV16 pseudovirus particles

in order to explore the mechanism of action of Qingre Jiedu prescription on transformed HeLa cell HPV. Method: Firstly

HPV16 pseudovirus particles were added to 293T/17 cells to establish an HPV pseudovirus infection model

so as to detect fluorescence intensity in vitro. Then this model was used to evaluate the effect of Qingre Jiedu prescription in resisting HPV16 infection in vitro and its dose-effect relationship of Qingre Jiedu prescription (20~1 000 g·L-1). Secondly

the HPV pseudovirus infection model was used to explore the action modes of Qingre Jiedu prescription (20 g·L-1) against HPV under such different conditions as pre-treating the cells

acting the cells simultaneously with virus and acting the cells after virus treatment. Finally

the mechanism of Qingre Jiedu prescription (3.33~20 g·L-1) was explored by Western blot. In brief

the oncogenic protein E7

extracellular signal-regulated kinase (ERK) and nuclear factor-κB (NF-κB) were extracted from HeLa cells HPV transformed after the action of Qingre Jiedu prescription and detected by Western blot. These proteins were compared with the corresponding protein expression levels in the blank group. Result: The results of experiment on HPV16 pseudovirus infection model showed that Qingre Jiedu prescription could significantly inhibit the infection of HPV16 pseudovirus in cells (P<0.05)

in an obvious concentration-dependent manner. The action modes of Qingre Jiedu prescription in the HPV16 pseudovirus infection model in vitro showed that Qingre Jiedu prescription could protect the cells

inhibit the absorption and post-virus infection process. And the drug can significantly prevent HPV16 infection because it could protect cells from the HPV16 infection (P<0.01). These results show that Qingre Jiedu prescription could protect cells from the HPV16 infection

inhibit the absorption and post-virus infection process. In addition

Qingre Jiedu prescription could obviously inhibit the expressions of oncoprotein E7

and ERK and NF-κB protein

so as to achieve the antitumor effect(P<0.05

P<0.01). Conclusion: Qingre Jiedu prescription has a better effect in inhibiting HPV virus

and the HPV16 pseudovirus infection experiment provides the innovative theory basis for the application of Qingre Jiedu prescription in anti-HPV and cervical cancer therapy.