Objective:

2

To observe the protective effect of Xiao Chaihutang and Wulingsan on residual renal function in patients with maintenance peritoneal dialysis (PD) and investigate its effect on peritoneal fibrosis and microinflammation.

Method:

2

The 65 patients with PD who were admitted to our hospital from June 2016 to June 2017 were enrolled in the study

and divided into control group (32 cases) and study group (33 cases) according to the random number table. The control group received routine treatment. The study group received routine treatment+ Xiao Chaihutang and Wulingsan. The fasting venous blood was taken before treatment and 3 months after treatment to measure serum creatinine (SCr) and urea nitrogen (BUN). Urine was collected; 24 hour urine volume was recorded; 24 h urine protein (24 h UP) was measured by colorimetry; glomerular filtration rate (eGFR) was calculated; residual renal function (RRF) was expressed with residual renal creatinine clearance. Inflammatory factors were detected by using chemiluminescence

including interleukin-6 (IL-6)

interleukin-8 (IL-8)

and tumor necrosis factor-

α

(TNF-

α

) levels. Serum Janus Kinase (JAK) 2 as well as signal transducer and activator of transcription (STAT) 3 levels were determined by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). E-cadherin and

α

-smooth muscle actin (

α

-SMA) levels were determined by Western blot.

Result:

2

Before treatment

there was no significant difference in the residual renal function between two groups. After treatment

the residual renal function of the study group was significantly better than that of control group (

P

<

0.05). After treatment

24 h UP of study group was lower than that of control group

and the daily average urine volume was higher than that of control group (

P

<

0.05). After treatment

the levels of IL-6

IL-8 and TNF-

α

in study group were lower than those in control group (

P

<

0.05); the levels of JAK2 and STAT3 in study group were significantly lower than those in control group (

P

<

0.01). The expression of E-cadherin protein in the dialysate of control group was significantly decreased after treatment (

P

<

0.01)

and the expression level of

α

-SMA protein was significantly increased after treatment (

P

<

0.01); however

study group had no significant changes in the expression of E-cadherin and

α

-SMA protein after treatment.

Conclusion:

2

Xiao Chaihutang and Wulingsan can protect the residual renal function of PD patients

and the mechanism may be related to the improvement of peritoneal fibrosis and the reduction of micro-inflammation of the body

showing a high application value.