Effect of Buyang Huanwu Tang on MPO, NE mRNA Expression, TNF-, IL-1, IL-6 and IL-17 in Mice with Rheumatoid Arthritis

ZHAN Jun-ping; MENG Qing-liang; MENG Wan-ting; GU Hui-min; WANG Hui-lian; ZUO Rui-ting; MA Jun-fu; MIAO Xi-yun; FAN Wei

doi:10.13422/j.cnki.syfjx.20182431

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Effect of Buyang Huanwu Tang on MPO, NE mRNA Expression, TNF-, IL-1, IL-6 and IL-17 in Mice with Rheumatoid Arthritis

更新时间：2024-09-23

- Effect of Buyang Huanwu Tang on MPO, NE mRNA Expression, TNF-, IL-1, IL-6 and IL-17 in Mice with Rheumatoid Arthritis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 24, Pages: 158-163(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20182431
  CLC： R285.5
- Published：2018
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ZHAN Jun-ping, MENG Qing-liang, MENG Wan-ting, et al. Effect of Buyang Huanwu Tang on MPO, NE mRNA Expression, TNF-, IL-1, IL-6 and IL-17 in Mice with Rheumatoid Arthritis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(24): 158-163.
DOI：

ZHAN Jun-ping, MENG Qing-liang, MENG Wan-ting, et al. Effect of Buyang Huanwu Tang on MPO, NE mRNA Expression, TNF-, IL-1, IL-6 and IL-17 in Mice with Rheumatoid Arthritis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(24): 158-163. DOI： 10.13422/j.cnki.syfjx.20182431.

摘要

目的：观察不同剂量的补阳还五汤对类风湿关节炎模型小鼠膝和踝关节滑膜中性粒细胞标志物髓过氧化物酶（myeloperoxidas，MPO），中性粒细胞弹性蛋白酶（neutrophil elastase，NE）mRNA表达及血清和关节液中肿瘤坏死因子-α（tumor necrosis factor-a

TNF-α），白细胞介素-1β（interleukin-1β

IL-1β），白细胞介素-6（interleukin-6

IL-6），白细胞介素-17（interleukin-17

IL-17）的影响。方法：将小鼠随机分为正常组，模型组，甲氨喋呤组（0.001 5 g·kg-1），补阳还五汤2.5，1.25，0.625 g·kg-1组。采用胶原诱导的方法制备类风湿关节炎小鼠模型，连续灌胃给药28 d后使用病理学方法分析小鼠治疗效果，使用酶联免疫吸附测定法（ELISA）检测小鼠血清及关节液中炎性因子的表达，使用实时荧光定量聚合酶链式反应（Real-time PCR）检测膝和踝关节滑膜中性粒细胞标志物蛋白MPO及NE mRNA表达。结果：与正常组比较，模型组小鼠关节明显肿胀（P<0.01），关节病理积分、血清及关节液中炎性因子水平，膝及踝关节中MPO，NE mRNA表达水平较明显升高（P<0.05，P<0.01）。与模型组比较，补阳还五汤组（2.5 g·kg-1）治疗28 d后小鼠小鼠关节肿胀明显减轻（P<0.05，P<0.01），关节病理积分、血清及关节液中炎性因子水平，膝及踝关节中MPO，NE mRNA表达水平较明显下降（P<0.05，P<0.01）。结论：适宜剂量的补阳还五汤可下调小鼠膝和踝关节滑膜中性粒细胞标志物蛋白MPO及NE mRNA表达水平，从而抑制中性粒细胞活性，降低血清和关节液中炎性因子TNF-α，IL-1β，IL-6和IL-17的水平。

Abstract

Objective: To explore the effect of Buyang Huanwu Tang with different doses on the expression of myeloperoxidas (MPO) and neutrophil elastase (NE) mRNA in synovial neutrophils of the knee and ankle

as well as the inflammatory factors tumor necrosis factor-α (TNF-α)

interleukin-1β(IL-1β)

interleukin-6 (IL-6)

and interleukin-17 (IL-17) in the serum and joint fluid of gouty arthritis (GA) mice. Method: The mice were randomly divided into normal control group

model group

the methotrexate group (0.001 5 g·kg-1)

Buyang Huanwu Tang high dose group (2.5 g·kg-1)

Buyang Huanwu Tang middle dose group (1.25 g·kg-1)

and Buyang Huanwu Tang low dose group(0.625 g·kg-1). Rheumatoid arthritis model was induced by collagen. After continuous lavage for 28 days

pathology method was used to analyze the treatment effects in mice. Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of inflammatory cytokines in serum and joint fluid; real time polymerase chain reaction (Real-time PCR) method was used to detect the mRNA expression of neutrophils marker protein MPO and NE in knee and ankle synovium. Result: As compared with normal group

joints were markedly swollen (P<0.01); joint pathological scores and inflammatory factor levels in serum and joint fluid

MPO

NE mRNA expression levels in knee and ankle joints were higher in model group (P<0.05

P<0.01). As compared with model group

joint swelling in mice was significantly relieved (P<0.05

P<0.01); joint pathological scores

inflammatory factor levels in serum and joint fluid

and MPO

NE mRNA expression levels in knee and ankle joints were obviously decreased in Buyang Huanwu Tang high dose group(P<0.05

P<0.01). Conclusion: Proper dose of Buyang Huanwu Tang may inhibit mRNA expression levels of knee and ankle synovial neutrophils marker protein MPO and NE

thus inhibiting activity of neutrophils

decreasing the inflammatory factors TNF-α

IL-1β

IL-6 and IL-17 in the serum and joint.

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