HPLC Fingerprint Analysis of Notopterygii Rhizoma et Radix with Different Commercial Specifications

Hui-qing GUO; Ya-qi LI; Zi-xuan WANG; Ze-kun ZHANG; Chang-hua MA

doi:10.13422/j.cnki.syfjx.20182490

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HPLC Fingerprint Analysis of Notopterygii Rhizoma et Radix with Different Commercial Specifications

Resource and Quality Evaluation | 更新时间：2021-02-09

- HPLC Fingerprint Analysis of Notopterygii Rhizoma et Radix with Different Commercial Specifications
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 184-188(2019)
- 作者机构：
  
  北京中医药大学　中药学院，北京　 102488
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182490
  CLC： R22;R28;C37;O657.7;R943.1
- Received：29 May 2018，
  
  Published Online：28 September 2018，
  
  Published：05 April 2019
- 稿件说明：
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Hui-qing GUO, Ya-qi LI, Zi-xuan WANG, et al. HPLC Fingerprint Analysis of Notopterygii Rhizoma et Radix with Different Commercial Specifications[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 184-188.
DOI：

Hui-qing GUO, Ya-qi LI, Zi-xuan WANG, et al. HPLC Fingerprint Analysis of Notopterygii Rhizoma et Radix with Different Commercial Specifications[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 184-188. DOI： 10.13422/j.cnki.syfjx.20182490.

摘要

目的：

建立不同商品规格羌活药材的HPLC指纹图谱，为羌活商品等级的划分和羌活药材质量的控制提供实验依据。

方法：

通过市场调查和文献研究，了解羌活药材商品状况，按照基原结合药材形态将羌活划分为蚕羌、条羌和大头羌3种商品规格。采用高效液相色谱-光电二极管阵列检测技术(HPLC-PDA)，以乙腈-0.3%乙酸水溶液为流动相进行梯度洗脱，建立不同商品规格羌活药材的指纹图谱；采用“中药色谱指纹图谱相似度评价系统”(2004A版)进行共有峰确认及相似度评价；通过SPSS 19.0软件采用主成分分析对指纹图谱进行模式识别。

结果：

分别建立了蚕羌、条羌和大头羌3种商品规格羌活药材的指纹图谱，从蚕羌中标定了22个共有峰，条羌中标定了23个共有峰，大头羌中标定了29个共有峰。相似度评价结合主成分分析结果显示相同商品规格羌活质量较为稳定，不同商品规格羌活的化学成分组成和含量差异显著。

结论：

所建立的指纹图谱方法可以很好地区别不同商品规格的羌活，可为羌活商品等级的划分及其质量控制提供参考。

Abstract

Objective:

To establish HPLC fingerprint of Notopterygii Rhizoma et Radix with different commercial specifications and to provide the basis for the division of commercial grades and the quality control of Notopterygii Rhizoma et Radix on the market.

Method:

The market investigation and literature research were used to understand the existing situation of Notopterygii Rhizoma et Radix goods.Notopterygii Rhizoma et Radix goods were divided into three commercial specifications according to the source and appearance

such as Canqiang

Tiaoqiang and Datouqiang.Fingerprint of Notopterygii Rhizoma et Radix with different commercial specifications was established by HPLC-PDA

the mobile phase was consisted of acetonitrile-0.3% acetic acid in a gradient elution mode.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(version of 2004A) was used to confirm the common peaks and evaluate the similarity.SPSS 19.0 statistical software was used to make principal component analysis(PCA) for HPLC fingerprint pattern.

Result:

The common mode of fingerprint for Canqiang

Tiaoqiang and Datouqiang were established separately.A total of 22 common peaks were marked in Canqiang

23 common peaks were marked in Tiaoqiang

29 common peaks were marked in Datouqiang.The result of similarity evaluation and PCA showed that the quality of Notopterygii Rhizoma et Radix with the same commercial specification was stable.There were great differences in chemical compositions and their contents among Notopterygii Rhizoma et Radix with different commercial specifications.

Conclusion:

The fingerprint method can well distinguish commercial specifications of Notopterygii Rhizoma et Radix

and it can provide the basis for the division of commercial grades and the quality control of Notopterygii Rhizoma et Radix.

关键词

Keywords

references

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