Spectrum-effect Relationship of Anti-inflammatory Active Components from Nonvolatile Fraction of Blumea balsamifera Based on Gray Correlation Analysis

Yang WANG; Xiao PENG; Yan-ling HE; Xu SUN; Xue MA; Guang-cheng WANG; Yan-yu LAN; Yong-jun LI

doi:10.13422/j.cnki.syfjx.20182492

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Spectrum-effect Relationship of Anti-inflammatory Active Components from Nonvolatile Fraction of Blumea balsamifera Based on Gray Correlation Analysis

Resource and Quality Evaluation | 更新时间：2021-02-09

- Spectrum-effect Relationship of Anti-inflammatory Active Components from Nonvolatile Fraction of Blumea balsamifera Based on Gray Correlation Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 4, Pages: 180-186(2019)
- 作者机构：
  
  贵州医科大学　民族药与中药开发应用教育部工程研究中心，药用植物功效与利用国家重点实验室，贵州省药物制剂重点实验室，药学院，贵阳　 550004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20182492
  CLC： R22;R24;R28;C37;O657.7
- Received：10 May 2018，
  
  Published Online：28 September 2018，
  
  Published：20 February 2019
- 稿件说明：
移动端阅览
Yang WANG, Xiao PENG, Yan-ling HE, et al. Spectrum-effect Relationship of Anti-inflammatory Active Components from Nonvolatile Fraction of Blumea balsamifera Based on Gray Correlation Analysis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 180-186.
DOI：

Yang WANG, Xiao PENG, Yan-ling HE, et al. Spectrum-effect Relationship of Anti-inflammatory Active Components from Nonvolatile Fraction of Blumea balsamifera Based on Gray Correlation Analysis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(4): 180-186. DOI： 10.13422/j.cnki.syfjx.20182492.

摘要

目的：

研究艾纳香非挥发性部位UPLC指纹图谱与抗炎药效之间的相关性，为明确艾纳香抗炎药效物质基础提供实验依据。

方法：

采用UPLC建立12批艾纳香非挥发性部位的指纹图谱，运用UPLC-Q-TOF-MS对艾纳香非挥发性部位的共有指纹峰进行指认，通过二甲苯致小鼠耳廓肿胀炎症模型获取相应的药效数据，通过灰色关联分析建立其谱效关系。

结果：

UPLC指纹图谱显示艾纳香非挥发性部位有14个共有峰，与抗炎药效的关联度处于0.717 1～0.550 5，各特征峰所代表的化学成分对其抗炎药效贡献的大小顺序为3号峰

9号峰

4号峰

11号峰

2号峰

1号峰

14号峰

7号峰

6号峰

5号峰

12号峰

8号峰

10号峰

13号峰，并指认了其中9个共有峰，对抗炎药效贡献较大的前2个共有峰(3号和9号)对应的化学成分分别为3-

-咖啡酰基奎宁酸和3，5-

-二咖啡酰基奎宁酸。

结论：

通过谱效关系研究获得了艾纳香非挥发性部位的抗炎药效物质群，其抗炎药效是多种成分共同作用的结果，明确咖啡酰基奎宁酸类化合物为艾纳香非挥发性部位发挥抗炎药效的主要物质基础。

Abstract

Objective:

To study the correlation between UPLC fingerprint of anti-inflammatory effect of active components from nonvolatile fraction of

Blumea balsamifera

and to provide the basis for clarifying the anti-inflammatory material basis of

balsamifera

Method:

UPLC was used to establish fingerprint of nonvolatile fraction of 12 batches of

balsamifera

and their common fingerprint peaks were identified by UPLC-Q-TOF-MS.The corresponding pharmacodynamic data were obtained by auricle swelling and inflammation model mice induced by xylene

and spectrum-effect relationship was established by gray correlation analysis.

Result:

A total of 14 common peaks in nonvolatile fraction of

balsamifera

were established by UPLC fingerprint and 9 common peaks of them were identified.The correlation between UPLC fingerprint and the anti-inflammatory activity was from 0.717 1 to 0.550 5.The contribution of chemical compositions represented by each characteristic peak to the anti-inflammatory efficacy was in the order of peak 3

peak 9

peak 4

peak 11

peak 2

peak 1

peak 14

peak 7

peak 6

peak 5

peak 12

peak 8

peak 10

peak 13

and the top two peaks with strong contribution to anti-inflammatory effect were peak 3 and peak 9

they were 3-

-caffeoylquinic acid and 3

5-di-

-caffeoylquinic acid identified by contrast reference substances

respectively.

Conclusion:

The active substances in nonvolatile fraction of

balsamifera

are obtained through the study on the relationship between spectrum and efficiency

and the anti-inflammatory efficacy of the nonvolatile fraction is the result of combination of various components.It is clear that the caffeoylquinic acid derivates act as predominant anti-inflammatory active substance of nonvolatile fraction of

balsamifera

关键词

Keywords

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