HPLC Characteristic Spectrum Optimization of Flavonoid Glycosides on <italic style="font-style: italic">Dendrobium officinale</italic> and Characteristics Analysis of Different Provenances

Zhi-yun LIANG; Zhen-shan XIE; Yue-chun HUANG; Yuan YUAN; Chu-juan ZHOU; Ya-wen WANG; Gang WEI

doi:10.13422/j.cnki.syfjx.20190112

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HPLC Characteristic Spectrum Optimization of Flavonoid Glycosides on Dendrobium officinale and Characteristics Analysis of Different Provenances

Topic on Germplasm Resources of Dendrobii Officinalis Caulis | 更新时间：2021-02-09

- HPLC Characteristic Spectrum Optimization of Flavonoid Glycosides on Dendrobium officinale and Characteristics Analysis of Different Provenances
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 22-28(2019)
- 作者机构：
  
  1.广州中医药大学　第一临床医学院，广州　 510006
  2.广州中医药大学　第一附属医院，广州　 510405
  3.中国中医科学院　中药资源中心，北京　 100700
  4.广州中医药大学　中药学院，广州　 510006
  5.韶关市铁皮石斛研究院，广东　韶关　 512005
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190112
  CLC： R284.1;R22;R2-031
- Received：24 August 2018，
  
  Published Online：19 October 2018，
  
  Published：05 January 2019
- 稿件说明：
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Zhi-yun LIANG, Zhen-shan XIE, Yue-chun HUANG, et al. HPLC Characteristic Spectrum Optimization of Flavonoid Glycosides on Dendrobium officinale and Characteristics Analysis of Different Provenances[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 22-28.
DOI：

Zhi-yun LIANG, Zhen-shan XIE, Yue-chun HUANG, et al. HPLC Characteristic Spectrum Optimization of Flavonoid Glycosides on Dendrobium officinale and Characteristics Analysis of Different Provenances[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 22-28. DOI： 10.13422/j.cnki.syfjx.20190112.

摘要

目的：

优化铁皮石斛黄酮苷类成分HPLC特征图谱分析方法，明确不同种源的共性成分与特异性成分。

方法：

色谱条件采用Kromasil 100-5 C

色谱柱；流动相四氢呋喃-乙腈-甲醇（10∶22∶5）-0.05%磷酸水溶液梯度洗脱；检测波长340 nm；柱温30 ℃；流速1.0 mL·min

－1

。

结果：

27批铁皮石斛共标示出13个黄酮类特征峰，鉴别了6个黄酮碳苷（新西兰牡荆苷Ⅱ，新西兰牡荆苷Ⅰ，夏佛塔苷，异夏佛塔苷，佛莱心苷，异佛莱心苷）与1个黄酮氧苷（芦丁）共7个特征峰。不同批次样品检出7～11个特征峰，其中新西兰牡荆苷Ⅱ为不同来源样品相对稳定的共有峰，芦丁、佛莱心苷与异佛莱心苷特征峰差异较大。根据特征峰的相对丰度，可将样品分为3大类，其中第Ⅰ类10批样品主要来源于广东、江西、福建、浙江（武义）等丹霞地貌（即“丹霞种”），以检出明显的芦丁强峰为特征；第Ⅱ类11批样品主要来源于云南、广西等地（包括了“云南广南种”“广西铁皮兰种”），以能检出佛莱心苷与异佛莱心苷特征峰为特征；而第Ⅲ类6批样品主要来源于浙江（即“浙江本地种”），以难以检出佛莱心苷与异佛莱心苷特征峰，检出不同程度的芦丁峰为特征。比较“丹霞种”与“广西铁皮兰种”的仿野生和大棚栽培铁皮石斛HPLC特征图谱，结果仿野生铁皮石斛中的特征峰在大棚栽培石斛中也能稳定检出，验证了铁皮石斛种源的可靠性。

结论：

所建立的分析方法能对铁皮石斛黄酮类特征峰达到较好的分离效果，重复性好，基本明确了不同种源铁皮石斛的共性及特异性黄酮苷类成分。提示新西兰牡荆苷Ⅱ适合作为特征图谱的参照物峰；可根据芦丁相对丰度或佛莱心苷与异佛莱心苷特征峰的检出及相对丰度作为铁皮石斛“丹霞种”“云南广南与广西铁皮兰种”与“浙江本地种”的判断依据。

Abstract

Objective:

To optimize the HPLC characteristic spectrum of flavonoid glycosides of

Dendrobium officinale

and identify the common and specific components of different provenances.

Method:

Kromasil 100-5 C

column was adopted

with tetrahydrofuran-acetonitrile-methanol (10∶22∶5)-0.05% phosphoric acid as mobile phase (gradient elution). The detection wavelength was 340 nm

the column temperature was 30 ℃

and the flow rate was 1.0 mL·min

-1

Result:

13 flavonoid characteristic peaks were marked in 27 batches of

officinale

and 7 characteristic peaks of 6 flavonoid

-glycosides (vicenin Ⅱ

vicenin Ⅰ

schaftoside

isoschaftoside

violanthin and isoviolanthin) and one flavonoid

-glycosides (rutin) was identified. 7-11 characteristic peaks were detected in different batches of samples. Among them

vicenin Ⅱ was a relatively stable common peak in different source samples

and the characteristic peaks of rutin

schaftoside and isoschaftoside were quite different. According to the relative abundance of the characteristic peaks

the samples could be divided into three categories. Among them

the first category had 10 batches of samples

which mainly came from Danxia landforms of Guangdong

Jiangxi

Fujian and Zhejiang (Wuyi) Province (which called " Danxia landform species" ) and characterized by detection of obvious peak of rutin. The second category had 11 batches of samples

which mainly came from Yunnan and Guangxi Province (which included " Yunnan Guangnan species" and " Guangxi Tiepilan species" ) and characterized by detection of violanthin and isoviolanthin. And the third category had 6 batches of samples

which were mainly derived from Zhejiang Province (which called " native species from Zhejiang" ) and characterized by detection of different degrees of rutin peak

but it was difficult to detect the characteristic peaks of violanthin and isoviolanthin. HPLC characteristic chromatograms of

officinale

in bionics wild cultivation and greenhouse of " Danxia landform species" and " Guangxi Tiepilan species" were compared. The results showed that the characteristic peaks in

officinale

planted in greenhouse could be detected stably

which verified the reliability of the source in

officinale

Conclusion:

The analytical method has a better separation effect on flavonoids of

officinale

with a good reproducibility. The commonness and specificities of flavonoid glycosides components of

officinale

from different categories have basically confirmed. This suggests that Vicenin Ⅱ is suitable to be a reference peak for characteristic chromatogram. Both the relative abundance of rutin and the detection or relative abundance of violanthin and isoviolanthin peaks could be used as a reference to judge the categories of

officinale

in " Danxia landform species" or " Tiepilan species from Yunnan

South Guangdong and Guangxi" or " native species from Zhejiang" .

关键词

Keywords

references

国家药典委员会．中华人民共和国药典．一部 [M]．北京：中国医药科技出版社， 2015 ： 282 .

魏刚，顺庆生，杨明志，等．石斛求真——中国药用石斛之历史、功效、真影与特征指纹图谱 [M]．成都：四川科学技术出版社， 2014 ： 27-32,53-56 .

周桂芬，陈素红，吕圭源．浙江省铁皮石斛黄酮类成分高效液相色谱指纹图谱的建立 [J]．中国药学杂志， 2013 ， 48 ( 15 ): 1261 - 1265 .

周桂芬，吕圭源．铁皮石斛叶中黄酮碳苷类成分的高效液相指纹图谱及指标成分的含量测定 [J]．中国药学杂志， 2012 ， 47 ( 11 ): 889 - 893 .

周桂芬，吕圭源．基于高效液相色谱-二极管阵列光谱检测-电喷雾离子化质谱联用鉴定铁皮石斛叶中8种黄酮碳苷化合物及裂解规律研究 [J]．中国药学杂志， 2012 ， 47 ( 1 ): 13 - 19 .

吕朝耕，杨健，康传志，等．铁皮石斛中10种黄酮类成分UPLC-MS/MS测定与多糖组成含量分析 [J]．中国实验方剂学杂志， 2017 ， 23 ( 17 ): 47 - 52 .

魏刚，顺庆生，黄月纯，等 . 3种铁皮石斛种源HPLC特征图谱比较研究 [J]．中药新药与临床药理， 2014 ， 25 ( 4 ): 467 - 471 .

黄月纯，谢镇山，任晋，等 . 3种种源铁皮石斛叶黄酮类成分HPLC特征图谱比较 [J]．中国实验方剂学杂志， 2015 ， 21 ( 24 ): 37 - 40 .

叶子，卢叶，薛亚甫，等．铁皮石斛专属性成分的分离制备及质量标准研究 [J]．中国中药杂志， 2016 ， 41 ( 13 ): 2481 - 2486 .

YE Z , DAI J R , ZHANG C G ,et al . Chemical differentiation of Dendrobium officinale and Dendrobium devonianum by using HPLC fingerprints,HPLC-ESI-MS,and HPTLC analyses [J]. Evid Based Complement Alternat Med , 2017 , doi: 10.1155/2017/8647212 http://doi.org/10.1155/2017/8647212 .

周春花，李运容，胡东南，等．基于“以广南者为佳”的广西铁皮石斛HPLC特征图谱研究 [J]．广东药科大学学报， 2018 ， 34 ( 3 ): 277 - 281 .

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HPLC Characteristic Spectrum Optimization of Flavonoid Glycosides on Dendrobium officinale and Characteristics Analysis of Different Provenances

DOI：10.13422/j.cnki.syfjx.20190112

摘要

Abstract

关键词

Keywords

references