Effect of Kangxianling Decoction on Renal Function and Kidney ECM Expression in Rats with Renal Fibrosis

Jing JI; Li-qun HE

doi:10.13422/j.cnki.syfjx.20190123

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Effect of Kangxianling Decoction on Renal Function and Kidney ECM Expression in Rats with Renal Fibrosis

Topic on Treatment of Renal Fibrosis with Kangxianling Formulas | 更新时间：2021-02-09

- Effect of Kangxianling Decoction on Renal Function and Kidney ECM Expression in Rats with Renal Fibrosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 63-68(2019)
- 作者机构：
  
  1.上海中医药大学，上海　 200123;
  2.上海中医药大学　附属曙光医院，上海　 200021
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190123
  CLC： R2-0;R22;R285.5;R289
- Received：10 August 2018，
  
  Published Online：18 October 2018，
  
  Published：05 January 2019
- 稿件说明：
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Jing JI, Li-qun HE. Effect of Kangxianling Decoction on Renal Function and Kidney ECM Expression in Rats with Renal Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 63-68.
DOI：

Jing JI, Li-qun HE. Effect of Kangxianling Decoction on Renal Function and Kidney ECM Expression in Rats with Renal Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 63-68. DOI： 10.13422/j.cnki.syfjx.20190123.

摘要

目的：

探讨抗纤灵方对5/6肾切除诱导肾纤维化模型大鼠肾功能及细胞外基质（extracellular matrix

ECM）表达的影响。

方法：

将50只SD大鼠随机分为正常组（

=7），假手术组（

=7），手术组（

=36）。手术组建立5/6肾切除肾纤维化模型，将造模成功的大鼠随机分为模型组、抗纤灵组、氯沙坦钾组。氯沙坦钾组、抗纤灵方组每日分别给予氯沙坦钾（33.3 g·kg

－1

）及抗纤灵方（21 g·kg

－1

）灌胃治疗，其余正常组、假手术组、模型组都用等体积的生理盐水干预，24周后测定各组肾功能，24 h尿量及24 h尿蛋白定量，免疫组化测定肾脏组织Ⅰ型胶原（ColⅠ），Ⅲ型胶原（ColⅢ）表达情况，蛋白免疫印迹法（Western blot）测定肾脏组织ColⅠ，ColⅢ蛋白表达水平。

结果：

与正常组和假手术组比较，模型组血肌酐，尿素氮，24 h尿量，24 h尿蛋白定量及肾组织ColⅠ，ColⅢ表达水平均明显升高（

0.01）；与模型组比较，抗纤灵方组和氯沙坦钾组血肌酐，尿素氮，24 h尿蛋白及肾组织ColⅠ，ColⅢ蛋白表达水平显著下降（

0.05，

0.01）。

结论：

抗纤灵方能下调肾脏组织ColⅠ，ColⅢ表达，从而抑制ECM的积聚，发挥延缓肾脏纤维化和保护肾功能的作用。

Abstract

Objective:

To investigate the effect of Kangxianling decoction on renal function and expression of extracellular matrix(ECM) in renal tissue of rats with renal fibrosis induced by 5/6 nephrectomy.

Method:

Totally 50 SD rats were randomly divided into control group (

=7)

sham-operation group (

=7)

operation group (

=36). The 5/6 nephrectomized renal fibrosis model was established in operation group. After successful modeling

rats were randomly divided into model group

Kangxianling group and losartan potassium group. The losartan potassium group and the Kangxianling group were given losartan potassium (33.3 g·kg

-1

) and Kangxianling decoction (21 g·kg

-1

) every day. The control group

sham-operation group and model group were all treated with equal volume of normal saline. Rats were put to death after 24 weeks of consecutive medication

and renal function

24 h urine volume and 24 h-Pro quantitation of each group were measured. The expressions of collagen Ⅰ(ColⅠ) and ColⅢ in renal tissues were determined by immunohistochemistry and Western blot.

Result:

Compared with the normal group and sham-operation group

serum creatinine(SCr)

blood urea nitrogen(BUN)

24 h urine volume

24 h-Pro quantitation

renal tissue ColⅠ and ColⅢ expression levels were significantly increased in the model group (

0.01). Compared with the model group

the levels of SCr

BUN

24 h-Pro quantitation

protein expression levels of renal tissue ColⅠ and ColⅢ were significantly decreased in the Kangxianling group and the losartan potassium group (

0.05

0.01).

Conclusion:

Kangxianling decoction can down-regulate the expressions of ColⅠ and ColⅢ in kidney tissue

there by inhibiting the accumulation of ECM

and exerting the effect in delaying renal fibrosis and protecting renal function.

关键词

Keywords

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