Effect of Shenghuitang on Learning Memory, Levels of IL-6, TNF-α and COX-2 in Hippocampus of Chronic Sleep Deprivation Mice

Chao YIN; Qiu-yun YOU; Mei-ya ZHANG; Li DING

doi:10.13422/j.cnki.syfjx.20190126

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Effect of Shenghuitang on Learning Memory, Levels of IL-6, TNF-α and COX-2 in Hippocampus of Chronic Sleep Deprivation Mice

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Shenghuitang on Learning Memory, Levels of IL-6, TNF-α and COX-2 in Hippocampus of Chronic Sleep Deprivation Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 96-100(2019)
- 作者机构：
  
  湖北中医药大学，武汉　 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190126
  CLC： R2-0;R22;R285.5;R289
- Received：12 June 2018，
  
  Published Online：18 October 2018，
  
  Published：05 January 2019
- 稿件说明：
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Chao YIN, Qiu-yun YOU, Mei-ya ZHANG, et al. Effect of Shenghuitang on Learning Memory, Levels of IL-6, TNF-α and COX-2 in Hippocampus of Chronic Sleep Deprivation Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 96-100.
DOI：

Chao YIN, Qiu-yun YOU, Mei-ya ZHANG, et al. Effect of Shenghuitang on Learning Memory, Levels of IL-6, TNF-α and COX-2 in Hippocampus of Chronic Sleep Deprivation Mice[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 96-100. DOI： 10.13422/j.cnki.syfjx.20190126.

摘要

目的：

通过观察生慧汤对慢性睡眠剥夺小鼠学习记忆及海马白细胞介素-6(interleukin-6，IL-6)，肿瘤坏死因子-

(tumor necrosis factor-

，TNF-

)，环氧化酶-2(cyclooxygenase-2，COX-2) mRNA表达的影响，探索生慧汤改善学习记忆能力的可能机制。

方法：

将实验小鼠随机分为睡眠剥夺模型组，正常组，褪黑素组（7.8×10

－4

g·kg

－1

·d

－1

），生慧汤高、中、低剂量组（54，27，13.5 g·kg

－1

·d

－1

），采用多平台水环境法建立小鼠慢性睡眠剥夺模型，睡眠剥夺28 d并灌胃给药。采用Morris水迷宫检测各组小鼠学习记忆能力；采用实时荧光定量聚合酶链式反应（Real-time PCR）法检测各组小鼠海马IL-6，TNF-

，COX-2 mRNA表达量。

结果：

Morris水迷宫试验结果显示，与正常组比较，模型组找到平台消耗时间与游泳总路程明显延长（

0.01），穿越平台次数与目标象限时间明显减少（

0.01），第1次抵原平台时间明显增加（

0.01）；与模型组比较，各给药组找到平台消耗时间与游泳总路程明显减少（

0.05，

0.01），穿越平台次数与目标象限时间明显增加（

0.05，

0.01），第1次抵原平台时间明显减少（

0.05，

0.01）。Real-time PCR检测结果显示，与正常组比较，模型组小鼠IL-6，TNF-

，COX-2 mRNA表达上调（

0.01）；与模型组比较，各给药组IL-6，TNF-

，COX-2 mRNA表达下调（

0.05，

0.01），生慧汤低剂量组TNF-

，COX-2 mRNA表达无显著性差异。

结论：

生慧汤可能通过降低海马中IL-6，TNF-

，COX-2 mRNA的表达来改善小鼠学习记忆能力。

Abstract

Objective:

To observe the effect of Shenghuitang on learning and memory and expressions of interleukin-6(IL-6)

tumor necrosis factor-

(TNF-

) and cyclooxygenase-2(COX-2) in hippocampus of chronic sleep deprived mice

in order to explore the possible mechanism of Shenghuitang in improving learning and memory ability.

Method:

Mice were randomly divided into sleep deprivation group

blank group

melatonin group(7.8×10

-4

g·kg

-1

·d

-1

)

high

middle and low-dose Shenghuitang groups(54

13.5 g·kg

-1

·d

-1

). The model of chronic sleep deprivation in mice was established using the " multi-platform water environment method" . 28 d sleep deprivation and intragastric administration were provided. Morris water maze was used to detect the learning and memory ability of mice in each group. Real time-PCR was used to detect mRNA expressions of IL-6

TNF-

and COX-2 in the hippocampus of each group.

Result:

The results of Morris water maze test showed that compared with the blank group

the total time spent on finding the platform and the total swimming distance of the model group were significantly prolonged (

0.01)

while the number of crossing platforms and the target quadrant were significantly reduced (

0.01). The time for the original platform was significantly increased (

0.01). Compared with the model group

the total time spent on finding the platform and the total swimming distance decreased significantly in each drug-administered group (

0.05

0.01) reduced

whereas the number of times for crossing the platform and the target quadrant increased significantly (

0.05

0.01). The time for the first arrival of the original platform was significantly reduced (

0.05

0.01). The results of RT-PCR showed that mRNA expressions of IL-6

TNF-

and COX-2 were increased in the model group compared with the blank group. Compared with the model group

mRNA expressions of IL-6

TNF-

and COX-2 were decreased in the treated group. COX-2 mRNA expression was down-regulated.

Conclusion:

Shenghuitang may improve the learning and memory ability of mice by decreasing mRNA expressions of IL-6

TNF-

and COX-2 in hippocampus.

关键词

Keywords

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