Effect of Wendantang on PI3K, Akt and GSK3β of Hippocampus in Rats with Schizophrenia

Jin-hua ZHU; Yi-yong XU; Hong-jiao WAN; Hao-xin SUN; Jia XU; Fan WU; Guo-pei ZHOU

doi:10.13422/j.cnki.syfjx.20190127

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Effect of Wendantang on PI3K, Akt and GSK3β of Hippocampus in Rats with Schizophrenia

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- Effect of Wendantang on PI3K, Akt and GSK3β of Hippocampus in Rats with Schizophrenia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 101-106(2019)
- 作者机构：
  
  江西中医药大学，南昌　 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190127
  CLC： R2-0;R22;R285.5;R289
- Received：29 June 2018，
  
  Published Online：19 October 2018，
  
  Published：05 January 2019
- 稿件说明：
移动端阅览
Jin-hua ZHU, Yi-yong XU, Hong-jiao WAN, et al. Effect of Wendantang on PI3K, Akt and GSK3β of Hippocampus in Rats with Schizophrenia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 101-106.
DOI：

Jin-hua ZHU, Yi-yong XU, Hong-jiao WAN, et al. Effect of Wendantang on PI3K, Akt and GSK3β of Hippocampus in Rats with Schizophrenia[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 101-106. DOI： 10.13422/j.cnki.syfjx.20190127.

摘要

目的：

研究温胆汤对精神分裂症模型鼠海马组织磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（Akt）/糖原合成酶激酶3

（GSK3

）信号通路的影响。

方法：

将60只大鼠随机分为6组，分别为正常组、模型组、氯氮平组、温胆汤高、中、低剂量组，每组10只。正常组和模型组灌胃生理盐水，氯氮平组灌胃氯氮平原药20 mg·kg

－1

；温胆汤高、中、低剂量组分别灌胃温胆汤生药40，20，10 g·kg

－1

；1次/d，共21 d。在最后1次给药2 h后，除正常组外，其余各组采用一次性左侧腹腔注射MK801 0.6 mg·kg

－1

，以诱发精神分裂症。观察并记录大鼠的刻板行为，3 d后，处死并取其海马组织待测。依照Sams Dodd和Hoffman标准对大鼠的刻板行为评分；蛋白免疫印迹法（Western blot）测定海马组织PI3K

Akt和GSK3

蛋白表达；实时荧光定量聚合酶链式反应（Real-time PCR）检测海马组织PI3K

Akt

GSK3

mRNA表达。

结果：

与正常组比较，模型组大鼠刻板行为评分显著升高（

0.01），海马组织PI3K

Akt

GSK3

蛋白和mRNA表达水平显著下降（

0.01）。与模型组比较，温胆汤给药组能明显降低大鼠刻板行为评分（

0.05），升高海马组织PI3K

Akt

GSK3

蛋白和mRNA表达水平（

0.05，

0.01）。

结论：

温胆汤通过改善精神分裂症模型鼠刻板行为、升高其海马组织PI3K

Akt

GSK3

的表达，调控PI3K/Akt/GSK3信号通路，从而达到治疗精神分裂症的目的。

Abstract

Objective:

To research the effect of Wendantang on phosphatidylinositol-3-kinases(PI3K)/protein kinase B(Akt)/glycogen synthase kinase 3

(GSK3

) signaling pathway of hippocampus in rats with schizophrenia.

Method:

Sixty SD rats were randomly divided six groups: normal group (A)

model group (B)

Clozapine group (C)

high-dosage group Wendantang (D)

medium-dosage Wendantang group (E) and low-dosage Wendantang group (F)

with 10 rats in each group. The rats of normal group and model group were given normal saline. The rats of Clozapine group were given 20 mg·kg

-1

Clozapine. The rats of high-dosage

medium-dosage

low-dosage Wendantang groups were respectively given 40

10 g·kg

-1

Wendantang

once a day

for 21 days.Two hours later after the last administration with Wendantang or saline

except for group A

groups B

F were intraperitoneally given MK-801 0.6 mg·kg

-1

to establish the rat schizophrenia model. After modeling

the changes in stereotyped behavior of each group were observed and recorded. After three days

the rats were put to death

and the hippocampus tissue were tested. According to Sams Dodd and Hoffman's standard

the stereotyped behavior of rats was scored. The protein expressions of PI3K

Akt and GSK3

of hippocampus were determined by Western blot. The mRNA expressions of PI3K

Akt and GSK3

of hippocampus were tested by Real-time PCR.

Result:

Compared with A

the stereotyped behavior score of the B was significantly increased (

0.01). The expressions of PI3K

Akt

GSK3

protein and mRNA of hippocampus were significantly decreased (

0.01) in rats of the B. Compared with the B

Wendantang administration groups could reduce the stereotyped behavior score(

0.05)

increased significantly the expressions of PI3K

Akt

GSK3

protein and PI3K

Akt and GSK3

mRNA of hippocampus (

0.05

0.01).

Conclusion:

Wendantang could regulate the PI3K/Akt/GSK3 signaling pathway by improving the stereotyped behavior and increasing the expressions of PI3K

Akt

GSK3

of hippocampus in rats with schizophrenia

so as to achieve the purpose of treating schizophrenia.

关键词

Keywords

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