Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity

Min-yi QIU; Xiao-yan JIN; Cai-juan ZHANG; Chi ZHANG; Min WANG; Yan-bing LU; Bao-sheng ZHAO; Yuan ZHANG; Xue-yong WANG

doi:10.13422/j.cnki.syfjx.20190130

您当前的位置：

首页 >

文章列表页 >

Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity

Pharmacology | 更新时间：2021-02-09

- Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 149-156(2019)
- 作者机构：
  
  1.北京中医药大学　中药学院，　北京中医药研究院，北京　 100029
  2.北京联合大学　生物化学工程学院，北京　 100023
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190130
  CLC： R28;R285;R285.5
- Received：28 July 2018，
  
  Published Online：17 October 2018，
  
  Published：05 January 2019
- 稿件说明：
移动端阅览
Min-yi QIU, Xiao-yan JIN, Cai-juan ZHANG, et al. Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 149-156.
DOI：

Min-yi QIU, Xiao-yan JIN, Cai-juan ZHANG, et al. Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 149-156. DOI： 10.13422/j.cnki.syfjx.20190130.

摘要

目的：

评价芭蕉芋RS3型抗性淀粉对肥胖型高脂血症小鼠的减肥降脂作用，并进行安全性评价。

方法：

KKAy小鼠高脂饲料喂养20周，建立肥胖型高脂血症模型，随机分为模型组，辛伐他汀组（4 mg·kg

－1

），RS3抗性淀粉高、低剂量组（2，1 g·kg

－1

），并以维持饲料喂养的小鼠为正常组。给药组分别灌胃相应药物，正常组及模型组给予等量去离子水。8周后，处死小鼠，检测血清中总胆固醇（TC），甘油三酯（TG），高密度脂蛋白胆固醇（HDL-C），低密度脂蛋白胆固醇（LDL-C）及天门冬氨酸氨基转移酶（AST），丙氨酸氨基转氨酶（ALT）的含量，精密称量脂肪质量，计算脂/体比、体脂率及Lee's指数，伊红-苏木素（HE）染色观察肝脏及脂肪组织的病理学变化；采用限量法评价RS3抗性淀粉的安全性，连续观察14 d，记录小鼠毒副反应情况。

结果：

RS3抗性淀粉高剂量能显著降低小鼠体质量、脂肪质量、体脂率、脂/体比、Lee's指数，以及血清中TC

LDL-C

AST

ALT水平（

0.05）。组织形态学检测显示，给予RS3抗性淀粉可以明显改善肝脏组织的脂肪病变情况，保肝作用明显，可以抑制脂肪细胞膨大，降低小鼠脂肪累积，以高剂量为优；急毒实验小鼠按36 g·kg

－1

剂量给药后，动物未出现中毒反应及死亡。

结论：

RS3型芭蕉芋抗性淀粉具有良好的减肥降脂作用，且以高剂量组为佳，最大给药量证明无毒副作用，临床常用剂量安全可靠。

Abstract

Objective:

To evaluate effect of

Canna edulis

type 3 resistant starch(RS3) on weight loss and lipid reduction in obese hyperlipidemia mice and acute toxicity in mice.

Method:

KKAy mice were fed with high-fat diet for 20 weeks to establish a hyperlipidemia model and then randomly divided into model group

positive group (4 mg·kg

-1

)

high-dose resistant starch group and low-dose resistant starch group (2

1 g·kg

-1

). Mice in normal group were fed with standard diet. The medication groups received corresponding drugs by gavage. Normal group and high-fat model group were given equal volume of deionized water. After 8 weeks

mice were put to death. The levels of total cholesterol (TC)

triglyceride (TG)

high-density lipoprotein cholesterol (HDL-C)

low-density lipoprotein cholesterol (LDL-C)

aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum of mice were measured

and weigh fat mass

fat/body ratio

body fat rate and Lee's index were calculated accurately. The pathological changes of liver and adipose tissue were observed byhematoxylin-eosin (HE). The acute toxicity of RS3 to mice was evaluated by limit test. The mice were continuously observed for 14 days

and the toxicity of mice was recorded.

Result:

The indicators of high-dose RS3 group were significantly reduced

such as body weight

fat mass

body fat rate

fat/body ratio

Lee's index

and serum TC

LDL-C

AST

ALT levels(

0.05). Histomorphometric examination showed that the administration of RS3 starch could significantly improve the fatty lesions of liver tissue

and the liver-protecting effect was obvious

which could still inhibit the expansion of fat cells and reduce the accumulation of fat in mice.Among them

the high-dose group wsa better; After the maximum dose of 36 g·kg

-1

was administered

no toxic reaction and death occurred in the animals.

Conclusion:

RS3-type

Canna Edulis

Resistant Starch has a good effect in reducing body weight and serum lipid

with a better effect in the high-dose group and no toxicity. And the commonly used clinical dose is safe and reliable.

关键词

Keywords

references

诸骏仁，高润霖，赵水平，等．中国成人血脂异常防治指南（2016年修订版） [J]．中国循环杂志， 2016 ， 31 （ 10 ）: 937 - 953 .

陈定宇，何凌，肖正华．肥胖伴高甘油三酯血症患者胰岛素抵抗的研究 [J]．中原医刊， 2006 ( 8 ): 1 - 2 .

原萌谦，刘志诚，徐斌，等．针灸治疗1528例肥胖并发高脂血症不同肥胖度患者疗效观察 [J]．中国针灸， 2016 ， 36 ( 8 ): 807 - 811 .

Paramsothy P , Knopp R , Bertoni A G ,et al . Combined hyperlipidemia in relation to race/ethnicity,obesity,and insulin resistance in the multi-ethnic study of atherosclerosis [J]. Metabolism , 2009 , 58 ( 2 ): 212 - 219 .

Garvey W T , Garber A J , Mechanick J I ,et al . American association of clinical endocrinologists and American college of endocrinology position statement on the 2014 adcanced framework for a new diagnosis of obesity as a chronic disease [J]. Endoc Pract , 2014 , 20 ( 9 ): 977 - 989 .

BAO L , BAI S , Borijihan G. Hypolipidemic effects of a new piperine derivative GB-N from piper longum in high-fat diet-fed rats [J]. Pharm Biol , 2012 , 50 ( 8 ): 962 - 967 .

Sajilata M G , Singhal R S , Kulkarni P R. Resistant starch-a review [J]. Compr Rev Food Sci F , 2010 , 5 ( 1 ): 1 - 17 .

Gocmen D , Dundar A N. Resistant starch as a novel food ingredient in human nutrition [J]. FASEB J , 2013 , 27 : 1065.26 .

Birt D F , Boylston T , Hendrich S ,et al . Resistant Starch:promise for improving human health [J]. Adv Nutr:Inter Rev Jour , 2013 , 4 ( 6 ): 587 - 601 .

Fuentes-Zaragoza E , Riquelme-Navarrete M J , Sánchez-Zapata E , et al . Resistant starch as functional ingredient: a review [J]. Food Res Int , 2010 , 43 ( 4 ): 931 - 942 .

余远江，何丽君，谢承孟，等．抗性淀粉的生理功效及其在食品工业中的应用研究进展 [J]．轻工科技， 2018 ， 34 ( 5 ): 43 - 45 .

YAO N , Paez A V , White P J. Structure and function of starch and resistant starch from corn with different doses of mutant amylose-extender and floury-1 alleles [J]. J Agric Food Chem , 2009 , 57 ( 5 ): 2040 - 2048 .

Sanz T , Salvador A , Fiszman S M. Evaluation of four types of resistant starch in muffin baking performance and relationship with batter rheology [J]. Eur Food Res Technol , 2008 , 227 ( 3 ): 813 - 819 .

Furukawa S , Fujita T , Shimabukuro M ,et al . Increased oxidative stress in obesity and its impact on metabolic syndrome [J]. J Clin Invest , 2004 , 114 ( 12 ): 1752 - 1761 .

Chandler P C , Viana J B , Oswald K D ,et al . Feeding response to melanocortin agonist predicts preference for andobesity from a high-fat diet [J]. Physiol Behav , 2005 , 85 ( 2 ): 221 - 230 .

ZHAO Y , PENG L , LU W ,et al . Effect of eclipta prostrata on lipid metabolism in hyperlipidemic animals [J]. Exp Gerontol , 2015 , 62 : 37 - 44 .

胡文兵，赵静，陈婷婷，等．青钱柳多糖对高脂血症小鼠的降血脂作用及机制初探 [J]．现代食品科技， 2015 ， 31 ( 11 ): 39 - 44 .

Aziz A A , Kenney L S , Goulet B ,et al . Dietary starch type affects body weight and glycemic control in freely fed but not energy-restricted obese rats [J]. J Nutr , 2009 , 139 ( 10 ): 1881 - 1889 .

GB 15193.3-2014，食品安全国家标准急性经口毒性试验 [S]．中华人民共和国国家卫生和计划生育委员会， 2014 ： 3 - 21 .

陈奇．中药药理研究方法学 [M]. 2版．北京：人民卫生出版社， 2006 ： 107 - 110 .

吴培赛，石松利，周红兵，等．蒙古扁桃药材不同提取物对高脂血症大鼠血脂、脂质过氧化和肝功能的影响 [J]．中国实验方剂学杂志， 2015 ， 21 ( 21 ): 113 - 117 .

李慧丽．肥胖、糖尿病、高脂血症与急性胰腺炎关系研究 [D]．济南：山东大学， 2016 .

范婷婷．荷叶生物碱类物质降脂减肥活性研究 [D]．杭州：浙江大学， 2013 .

何明，涂长春，黄起壬，等． Lee's指数用于评价成年大鼠肥胖程度的探讨 [J]．中国临床药理学与治疗学杂志， 1997 ( 3 ): 177 - 179 .

余远江，何丽君，谢承孟，等．抗性淀粉的生理功效及其在食品工业中的应用研究进展 [J]．轻工科技， 2018 ， 34 ( 5 ): 43 - 45 .

HAN K H , Fukushima M , Kato T ,et al . Enzyme-resistant fractions of beans lowered serum cholesterol and increased sterol excretions and hepatic mRNA levels in rats [J]. Lipids , 2003 , 38 ( 9 ): 919 - 924 .

李敏，杨晓光，朴建华．抗性淀粉生理功能的研究进展 [J]．卫生研究， 2008 ， 37 ( 5 ): 640 - 643 .

张蕾，张琪，游云，等．基于代谢组学技术探讨高脂血症及动脉粥样硬化痰瘀证候的演变规律 [J]．中国中西医结合杂志， 2015 ， 35 ( 7 ): 823 - 833 .

唐琪晶，陈素红，潘丹丹，等．白术精提物对代谢性高脂血症大鼠的药效及机制研究 [J]．中国中药杂志， 2015 ， 40 ( 9 ): 1803 - 1807 .

冷雪，贾连群，杨关林，等．化瘀祛痰方对脾虚型高脂血症大鼠肝脏SREBP-2信号通路的干预作用 [J]．中国中西医结合杂志， 2015 ， 35 ( 3 ): 320 - 326 .

陈娟，邓军，张宇燕，等．丹参素对高脂血症大鼠脂代谢调节机制研究 [J]．中国中药杂志， 2015 ， 40 ( 2 ): 313 - 317 .

叶振南，李楠，盛丹丹，等．青钱柳多糖对高脂血症大鼠血脂及抗脂质过氧化作用的影响 [J]．现代食品科技， 2014 ， 30 ( 4 ): 1 - 5,20 .

吴燕丹． TC/HDL-C, LDL-C/HDL-C, TG/HDL-C与冠心病不同程度相关性 [J]．临床误诊误治， 2014 ， 27 ( 6 ): 64 - 67 .

Englyst H N , Cummings J H. Non-starch polysaccharides (dietary fiber) and resistant starch [J]. Adv Exp Med Biol , 1990 , 270 ( 6 ): 205 - 225 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical Efficacy of Zhuyuwan in Treatment of Hyperlipidemia with Syndrome of Phlegm Turbidity and Obstruction

Zhuyuwan in Treatment of Hyperlipidemia Complicated with Carotid Atherosclerosis： A Randomized Controlled Trial

Mechanism of Wendantang in Intervention of ApoE^-/- Hyperlipidemic Mice Based on Liver Metabolomics

Polygonati Odorati Rhizoma Polysaccharides Ameliorate Hyperlipidemia in Mice by Regulating Gut Microbiota

Wendantang Regulates Energy Metabolism in Treatment of Myocardial Ischemia via SIRT3/PGC-1α Pathway

Related Author

YANG Lele

LUO Danmei

CHEN Jiao

ZHANG Xiaobo

SONG Wei

ZHU Wenyu

ZHOU Xin

LI Xueping

Related Institution

Chengdu University of TCM

Hospital of Chengdu University of Traditional Chinese Medicine （TCM）

Hospital of Chengdu University of TCM

Chengdu University of Traditional Chinese Medicine（TCM）

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory/Jiangxi Province Key Laboratory of Biological Etiologies of TCM/First-Level Discipline Platform of Chinese and Western Integrative Medicine/Experimental Animal and Scientific Technology Center of Jiangxi University of Chinese Medicine/School of Pharmacy， Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰