Establishment of DNA Fingerprints and Genetic Similarities Analysis of <italic style="font-style: italic">Dendrobium officinale</italic>

Wen-xia YU; Zhou-xi LEI; Yuan YUAN; Yu-lin WU; Qing-sheng SHUN; Gang WEI; Liu-ping HU; Ju-ting HU; Lu-qi HUANG

doi:10.13422/j.cnki.syfjx.20190161

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Establishment of DNA Fingerprints and Genetic Similarities Analysis of Dendrobium officinale

Topic on Germplasm Resources of Dendrobii Officinalis Caulis | 更新时间：2021-02-09

- Establishment of DNA Fingerprints and Genetic Similarities Analysis of Dendrobium officinale
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 16-21(2019)
- 作者机构：
  
  1.广州中医药大学　中药学院，广州　 510006
  2.广东省中药研究所，广州　 510006
  3.中国中医科学院　中药资源中心，北京　 100700
  4.上海健康医学院，上海　 200237
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190161
  CLC： R284.1;R282.5;R289;R22
- Received：31 August 2018，
  
  Published：05 January 2019
- 稿件说明：
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Wen-xia YU, Zhou-xi LEI, Yuan YUAN, et al. Establishment of DNA Fingerprints and Genetic Similarities Analysis of Dendrobium officinale[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 16-21.
DOI：

Wen-xia YU, Zhou-xi LEI, Yuan YUAN, et al. Establishment of DNA Fingerprints and Genetic Similarities Analysis of Dendrobium officinale[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 16-21. DOI： 10.13422/j.cnki.syfjx.20190161.

摘要

该研究利用中国中医科学院中药资源中心筛选出的4对简单重复序列（simple sequence repeats

SSR）引物对铁皮石斛主产区9个产地29个居群的铁皮石斛样品进行检测，4对SSR引物分别扩增出5，7，4，3条多态性带，根据不同居群的SSR指纹图谱构建DNA身份证。聚类分析可将来自29个居群样本分成四大类，且表现出较好的地域相关性，其中来自云南、贵州、四川的铁皮石斛样品聚为一类，来自安徽和广西的铁皮石斛单独聚为一类，来自广东丹霞、浙江永康、浙江乐清及泰宁的样品聚为一类。采用PopGene（version 1.32）软件包对29个铁皮石斛居群进行遗传相似性分析，相似系数变异在0.403 4～1.0。基于遗传相似系数可将不同居群铁皮石斛的遗传一致性分成A

C共3个等级，地理位置相近或生长地貌相似的居群遗传相似性系数较高，表明其遗传背景较为一致。本研究为铁皮石斛的品种鉴别及优良品种选育提供参考信息。

Abstract

This study adopted the 4 pairs of simple sequence repeats(SSR)primers selected by National Resource Center for Chinese Meteria Medica (CACMS) to detect 29 populations of

Dendrobium officinale

samples from 9 main places of production; 5

4 and 3 polymorphism bands were amplified from these 4 pairs of SSR primers. The DNA identities of different populations were constructed by SSR. The 29

officinale

populations could be divided into 4 classes. The clustering result was related to the places of production. Samples from Yunnan

Guizhou

Sichuan provinces were classified into one category

while samples from Anhui and Guangxi provinces were classified into another category. Samples from Guangdong Danxia

Zhejiang Yongkang

Zhejiang Leqing and Taining belonged to a category. PopGene (version 1.32) software was applied to calculate the genetic similarity of the 29

officinale

populations. The similarities was between 0.403 4 and 1.0.Based on the genetic similarity

the genetic consistency included three classes

B and C. Samples with a similar geographical location and landform environment have higher genetic similarities

which indicate the same genetic background. This paper provides reference information to study the identification

selection and breeding of good varieties.

关键词

Keywords

references

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Related Author

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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Nanyang Vocational College of Agriculture

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Establishment of DNA Fingerprints and Genetic Similarities Analysis of Dendrobium officinale

DOI：10.13422/j.cnki.syfjx.20190161

摘要

Abstract

关键词

Keywords

references