Determination of Contents of Five Components in Descurainiae Semen Before and After Being Processed by HPLC

Han-yan LUO; Xian-kuo YU; Xiao ZHANG; Ya-qi LU; Ri-xin GUO; Li-ying TANG; Zhu-ju WANG

doi:10.13422/j.cnki.syfjx.20190201

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Determination of Contents of Five Components in Descurainiae Semen Before and After Being Processed by HPLC

Pharmacy and Processing | 更新时间：2021-02-09

- Determination of Contents of Five Components in Descurainiae Semen Before and After Being Processed by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 10, Pages: 115-120(2019)
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.河南中医药大学　药学院，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190201
  CLC： R22; R943.1; R28; O657.7
- Received：03 August 2018，
  
  Published Online：02 November 2018，
  
  Published：20 May 2019
- 稿件说明：
移动端阅览
Han-yan LUO, Xian-kuo YU, Xiao ZHANG, et al. Determination of Contents of Five Components in Descurainiae Semen Before and After Being Processed by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 115-120.
DOI：

Han-yan LUO, Xian-kuo YU, Xiao ZHANG, et al. Determination of Contents of Five Components in Descurainiae Semen Before and After Being Processed by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 115-120. DOI： 10.13422/j.cnki.syfjx.20190201.

摘要

目的：

建立葶苈子中5个指标成分的HPLC含量测定方法，研究炮制前后葶苈子中5个成分的含量变化规律。

方法：

采用HPLC同时测定槲皮素-3-

-葡萄糖-7-

-龙胆双糖苷(QGG)，芥子酸，槲皮素-3-

-吡喃葡萄糖苷(QG)，异鼠李素-3-

-吡喃葡萄糖苷(IG)和1，2-

-二-芥子酰基-

-吡喃葡萄糖苷(SG)共5个成分的含量，分析炮制前后的含量变化规律及其原因。色谱条件为Waters Symmetry

色谱柱(4.6 mm×250 mm，5 μm)，流动相乙腈(A)-1%乙酸水溶液(B)梯度洗脱(0～5 min，5%～10%A；5～15 min，10%～13%A；15～23 min，13%～20%A；23～43 min，20%～25%A；43～46 min，25%A；46～55 min，25%～40%A；55～60 min，40%A)，流速1 mL·min

－1

，检测波长265 nm，进样量10 μL，柱温30 ℃。

结果：

上述5个成分炮制前的质量分数分别为0.114 3%，0.041 6%，0.036 2%，0.022 6%，0.097 6%；炮制后的质量分数分别为0.107 4%，0.011 3%，0.034 2%，0.021 9%，0.058 9%；5个成分的质量分数分别下降了6.04%，72.84%，5.52%，3.10%，39.65%。

结论：

葶苈子中所含的这5个成分含量经炮制后均有不同程度的降低，其中苯丙素类成分炮制后含量明显下降，而黄酮苷类成分的含量变化则不明显。

Abstract

Objective:

HPLC for the determination of five components in Descurainiae Semen was established to investigate the change rule of contents of five components in the herb before and after being processed.

Method:

The contents of quercetin-3-

-glucose-7-

-gentiobioside(QGG)

sinapic acid

quercetin-3-

-glucopyranoside(QG)

isorhamnetin-3-

-glucopyranoside(IG) and 1

2-di-

-sinapoyl-

-glucopyranose(SG) was determined simultaneously by HPLC

the change rule of contents of these components before and after processing and its reasons were analyzed.Waters Symmetry

column(4.6 mm×250 mm

5 μm) was employed

and the mobile phase was acetonitrile(A)-1% acetic acid aqueous solution(B) for gradient elution(0-5 min

5%-10%A; 5-15 min

10%-13%A; 15-23 min

13%-20%A; 23-43 min

20%-25%A; 43-46 min

25%A; 46-55 min

25%-40%A; 55-60 min

40%A). The flow rate was 1 mL·min

-1

.The detection wavelength was set at 265 nm

the injection volume was 10 μL

and the column temperature was 30 ℃.

Result:

Contents of the above five components before processing were 0.114 3%

0.041 6%

0.036 2%

0.022 6% and 0.097 6%; after processing

the contents of these five components turn into 0.107 4%

0.011 3%

0.034 2%

0.021 9% and 0.058 9%; among them

the contents of these five components decreased by 6.04%

72.84%

5.52%

3.10% and 39.65%

respectively.

Conclusion:

The contents of these five components in Descurainiae Semen is reduced to varying degrees after processing.The contents of phenylpropanoids decrease significantly

while the contents of flavonoid glycosides do not change significantly.

关键词

Keywords

references

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