Discussion About Application of Thin Layer Chromatography in Current Quality Standard Control

Zhe DENG; Wen-guang JING; An LIU

doi:10.13422/j.cnki.syfjx.20190202

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Discussion About Application of Thin Layer Chromatography in Current Quality Standard Control

Academic Discussion | 更新时间：2021-02-09

- Discussion About Application of Thin Layer Chromatography in Current Quality Standard Control
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 7, Pages: 201-206(2019)
- 作者机构：
  
  1.中国中医科学院　中药研究所，北京　 100700；
  2.中国中药有限公司，北京　 102600
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190202
  CLC： R22;R28;G353.11
- Received：07 August 2018，
  
  Published Online：02 November 2018，
  
  Published：05 April 2019
- 稿件说明：
移动端阅览
Zhe DENG, Wen-guang JING, An LIU. Discussion About Application of Thin Layer Chromatography in Current Quality Standard Control[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 201-206.
DOI：

Zhe DENG, Wen-guang JING, An LIU. Discussion About Application of Thin Layer Chromatography in Current Quality Standard Control[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(7): 201-206. DOI： 10.13422/j.cnki.syfjx.20190202.

摘要

薄层色谱鉴别在历版《中国药典》中的应用经历了从无到有、从少到多的过程；而薄层扫描含量测定在最近几版《中国药典》中的应用比例逐渐降低。随着对中药质量标准体系要求的进一步提高，薄层色谱法的不足之处陆续显现，如仪器普及率低、设备并不简单、结果重复性和稳定性较差、鉴别速度及准确性不及高效液相色谱法、展开剂毒性大等，逐渐不合时宜。在制定中药质量标准时，研究者不应该墨守成规，薄层色谱鉴别也不应该是雷打不动的定性鉴别必备选项。高效液相色谱法具备完全取代薄层色谱法的可行性，薄层色谱法可作为高效液相色谱法的补充。为充分降低检测成本、缩短检测周期、提高鉴别效率，笔者建议中药质量标准体系应该大幅减少薄层色谱鉴别方法的应用，增加高效液相特征图谱鉴定，尽量做到“一个条件，一张图谱”；除非确有必要，《中国药典》等国家质量标准体系应将薄层色谱鉴别作为推荐方法，而非强制标准。

Abstract

The application of thin layer chromatography(TLC) in

Chinese Pharmacopoeia

experienced from scratch to less to more

but the proportion of thin layer chromatographic scanning used in recent editions of

Chinese Pharmacopoeia

has decreased.With the further improvement of the quality standard system of traditional Chinese medicine(TCM)

the shortcomings of TLC identification methods have been gradually revealed

such as the low penetration rate of instrument

poor repeatability and stability of the results

lower identification speed than high performance liquid chromatography(HPLC) and so on.Thus it gradually became untimely.In the process of formulating quality standard control of TCM

researchers should not be conformist

and TLC identification should not be a necessary choice for the qualitative identification.And HPLC has the possibility to completely replace TLC

but TLC can be used as a supplement to HPLC.In order to fully lower testing cost

shorten testing cycle

improve the efficiency of identification

we suggested quality standard system of TCM should be sharply reduced TLC identification method and increased identification of HPLC characteristic spectrum; and try to do "one condition

one map" . Unless absolutely necessary

national quality standards(such as

Chinese Pharmacopoeia

) should use TLC identification as a recommended method rather than a mandatory standard.

关键词

Keywords

references

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