Effect of Different Processing Methods on Types and Contents of Chemical Constituents in Codonopsis Radix from Shanxi

Rui-yan LI; Li-na LAI; Lei NIE; Xiao-xia YAN

doi:10.13422/j.cnki.syfjx.20190206

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Effect of Different Processing Methods on Types and Contents of Chemical Constituents in Codonopsis Radix from Shanxi

Pharmacy and Processing | 更新时间：2021-02-09

- Effect of Different Processing Methods on Types and Contents of Chemical Constituents in Codonopsis Radix from Shanxi
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 11, Pages: 133-138(2019)
- 作者机构：
  
  1.长治医学院，山西　长治　 046000；
  2.山西振东制药股份有限公司，山西　长治　 047100
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190206
  CLC： R22; R28; O657.7; R943.1
- Received：17 August 2018，
  
  Published Online：26 October 2018，
  
  Published：05 June 2019
- 稿件说明：
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Rui-yan LI, Li-na LAI, Lei NIE, et al. Effect of Different Processing Methods on Types and Contents of Chemical Constituents in Codonopsis Radix from Shanxi[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 133-138.
DOI：

Rui-yan LI, Li-na LAI, Lei NIE, et al. Effect of Different Processing Methods on Types and Contents of Chemical Constituents in Codonopsis Radix from Shanxi[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 133-138. DOI： 10.13422/j.cnki.syfjx.20190206.

摘要

目的：

建立潞党参生品、米炒潞党参和蜜炙潞党参的HPLC指纹图谱并进行化学成分含量测定，分析炮制前后潞党参化学成分种类及含量的变化情况。

方法：

HPLC指纹图谱的建立采用Agilent ZORBAX SB-C

色谱柱(4.6 mm×250 mm，5 μm)，乙腈-0.5%磷酸水溶液为流动相梯度洗脱，柱温30 ℃，检测波长220 nm。

结果：

10批潞党参生品及其炮制品的指纹图谱与相应对照指纹图谱的相似度均

0.90。在潞党参生品、米炒潞党参和蜜炙潞党参中，党参炔苷质量分数分别为(0.33±0.049)，(0.24±0.034)，(0.18±0.047) mg·g

－1

；苍术内酯Ⅲ质量分数分别为(0.20±0.046)，(0.40±0.046)，(0.31±0.060) mg·g

－1

；5-羟甲基糠醛质量分数分别为(0.74±0.16)，(1.45±0.19)，(1.54±0.12) mg·g

－1

。

结论：

不同炮制方法对潞党参中化学成分种类的影响较小，但对有些化学成分含量的影响较大。

Abstract

Objective:

To establish the HPLC fingerprint of raw products

rice-processed products and honey-processed products of Codonopsis Radix from Shanxi

and establish determination of their chemical constituents

which was used to analyze the changes of types and contents of chemical constituents in Codonopsis Radix from Shanxi before and after processing.

Method:

Agilent ZORBAX SB-C

column(4.6 mm×250 mm

5 μm) was adopted

the separation process was carried out using a binary gradient elution system composed of 0.5% phosphoric acid aqueous solution and acetonitrile

the column temperature was 30 ℃ and the detection wavelength was 220 nm.

Result:

Compared with the corresponding reference fingerprint

the similarities of HPLC fingerprint of 10 batches of raw products and processed products were

0.90.In raw products

rice-processed products and honey-processed products of Codonopsis Radix from Shanxi

the contents of lobetyolin were (0.33±0.049)

(0.24±0.034)

(0.18±0.047) mg·g

-1

the contents of atractylenolide Ⅲ were (0.20±0.046)

(0.40±0.046)

(0.31±0.060) mg·g

-1

the contents of 5-hydroxymethylfurfural(5-HMF) were (0.74±0.16)

(1.45±0.19)

(1.54±0.12) mg·g

-1

respectively.

Conclusion:

Different processing methods have little effect on types of chemical constituents in Codonopsis Radix from Shanxi

but have great effect on the contents of some chemical constituents.

关键词

Keywords

references

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