Preparation of Magnetic Solid Lipid Nanoparticles Co-loaded Quercetin and Resveratrol and Evaluation of Its Antitumor Effect

Qian DU; Yang TANG; Jian-qiu CHEN; Fang-nan LYU; Jin REN

doi:10.13422/j.cnki.syfjx.20190209

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Preparation of Magnetic Solid Lipid Nanoparticles Co-loaded Quercetin and Resveratrol and Evaluation of Its Antitumor Effect

Pharmacy and Processing | 更新时间：2021-02-09

- Preparation of Magnetic Solid Lipid Nanoparticles Co-loaded Quercetin and Resveratrol and Evaluation of Its Antitumor Effect
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 10, Pages: 109-114(2019)
- 作者机构：
  
  徐州医科大学　药学院，江苏　徐州　 221004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190209
  CLC： R22; R24; R28; C37; TS193
- Received：04 July 2018，
  
  Published Online：02 November 2018，
  
  Published：20 May 2019
- 稿件说明：
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Qian DU, Yang TANG, Jian-qiu CHEN, et al. Preparation of Magnetic Solid Lipid Nanoparticles Co-loaded Quercetin and Resveratrol and Evaluation of Its Antitumor Effect[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 109-114.
DOI：

Qian DU, Yang TANG, Jian-qiu CHEN, et al. Preparation of Magnetic Solid Lipid Nanoparticles Co-loaded Quercetin and Resveratrol and Evaluation of Its Antitumor Effect[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 109-114. DOI： 10.13422/j.cnki.syfjx.20190209.

摘要

目的：

制备槲皮素和白藜芦醇共载磁性固体脂质纳米粒(QR-MSLN)，探索合理表征方法并考察其对小鼠肝癌H22移植性肿瘤的抑瘤作用。

方法：

制备油酸表面修饰的磁性Fe

(OA-Fe

)，采用红外光谱分析进行结构确证；采用乳化超声分散法制备QR-MSLN，分别采用透射电子显微镜、纳米粒径-Zeta电位测定仪观测其形态与粒径，邻二氮菲分光光度法测定制剂中铁含量，超滤离心法测定2种成分的包封率，振动样品磁强计测定其饱和磁化强度，透析法研究其体外释药行为；建立小鼠肝癌细胞(H22)移植瘤模型，评价在外加磁场下QR-MSLN对移植瘤的抑制作用。

结果：

QR-MSLN形态圆整，内部可见载有黑色磁性微粒，粒径(171.9±2.2) nm，铁质量浓度(1.40±0.46) g·L

－1

，饱和磁化强度7.75 A·m

·kg

－1

，具有超顺磁性。槲皮素和白藜芦醇的包封率分别为99.10%，80.83%。与槲皮素和白藜芦醇原药、载药固体脂质纳米粒相比，QR-MSLN的肿瘤抑制率显著增高(

0.01)。

结论：

制得的QR-MSLN粒径均匀、磁响应性好，对小鼠H22移植瘤具有明显的抑制作用。

Abstract

Objective:

To prepare magnetic solid lipid nanoparticles co-loaded quercetin and resveratrol(QR-MSLN)

develop the reasonable characterization method

and investigate its inhibitory effect on transplanted hepatocarcinoma H22 tumor in mice.

Method:

Magnetic Fe

particles coated with oleic acid(OA-Fe

) were synthesized and its structure was confirmed by Fourier transform infrared spectrometer(FT-IR). QR-MSLN was prepared by emulsion ultrasonic dispersion method

its morphology was examined by transmission electron microscopy

its particle size was determined by laser particle sizer.Concentration of Fe in the preparation was determined by phenanthroline spectrophotometry.The entrapment efficiency

saturation magnetization

in vitro

release behavior were investigated by ultrafiltration centrifugation

vibrating sample magnetometer(VSM) and dialysis method

respectively.Mouse tumor model transplanted with hepatoma H22 ascites tumor was established and antitumor effect of QR-MSLN on H22 bearing mice were observed in the presence of an applied magnetic field.

Result:

Morphology of QR-MSLN was round

and black magnetic particles could be observed inside it

its particle size was (171.9±2.2) nm

the concentration of Fe was (1.40±0.46) g·L

-1

.The preparation exhibited apparent superparamagnetism and the saturation magnetization was 7.75 A·m

·kg

-1

.The entrapment efficiencies of quercetin and resveratrol in QR-MSLN were 99.10% and 80.83%

respectively.QR-MSLN had a significantly higher effect of tumor inhibition than SLN(containing quercetin and resveratrol) and free drug(

0.01).

Conclusion:

QR-MSLN has uniform particle size and good magnetic response

and shows remarkable antitumor effect on H22 bearing mice.

关键词

Keywords

references

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