Research on Profile Differences of Chemical Components in Magnoliae Officinalis Cortex from Different Varieties and Habitats by LC-TOF-MS

Fang LIU; Qi-juan LI; Qiao LIU; Ming FANG; Ya-ji XU; Yu-lian GAO; Hui-ling HU; Ying-fang WEI; Zhan-guo WANG

doi:10.13422/j.cnki.syfjx.20190210

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Research on Profile Differences of Chemical Components in Magnoliae Officinalis Cortex from Different Varieties and Habitats by LC-TOF-MS

Pharmacy and Processing | 更新时间：2021-02-09

- Research on Profile Differences of Chemical Components in Magnoliae Officinalis Cortex from Different Varieties and Habitats by LC-TOF-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 10, Pages: 121-126(2019)
- 作者机构：
  
  1.成都中医药大学　药学院，中药材标准化教育部重点实验室，中药资源系统研究与开发利用省部共建国家重点实验室培育基地，成都　 611137；
  2.成都大学　医学院，护理学院，代谢组学协同创新实验室，成都　 610106
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190210
  CLC： R22; R931; R28; C37; O657
- Received：15 May 2018，
  
  Published Online：29 October 2018，
  
  Published：20 May 2019
- 稿件说明：
移动端阅览
Fang LIU, Qi-juan LI, Qiao LIU, et al. Research on Profile Differences of Chemical Components in Magnoliae Officinalis Cortex from Different Varieties and Habitats by LC-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 121-126.
DOI：

Fang LIU, Qi-juan LI, Qiao LIU, et al. Research on Profile Differences of Chemical Components in Magnoliae Officinalis Cortex from Different Varieties and Habitats by LC-TOF-MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(10): 121-126. DOI： 10.13422/j.cnki.syfjx.20190210.

摘要

目的：

对不同品种和不同产地厚朴的化学物质进行轮廓谱差异研究，筛选并鉴定影响该药材质量差异的特征性成分。

方法：

采用液相色谱-飞行时间-质谱联用技术(LC-TOF-MS)分析并获得不同品种和不同产地厚朴的色谱图数据集，运用变量统计分析方法比较不同品种和不同产地厚朴的化学轮廓差异，筛选出导致这些差异的特征性化学成分，并对其进行质谱分析和比对。

结果：

分析并鉴定出了11个特征峰，包括厚朴酚、和厚朴酚、石竹厚朴酚、厚朴碱、木兰花碱、鹅掌楸碱、罗默碱、海罂粟碱、阿西米洛宾、塔斯品碱、观音莲明碱。

结论：

LC-TOF-MS可简便、快速地对不同产地和不同品种厚朴中化学物质进行轮廓差异性研究，为后期厚朴品质评价和药效物质基础研究提供了一定的实验依据。

Abstract

Objective:

To study on the differences of profile spectra among chemical components in Magnoliae Officinalis Cortex from different varieties and habitats

and to screen and identify the characteristic components affecting the quality difference of this herb.

Method:

The chromatogram data sets of Magnoliae Officinalis Cortex from different varieties and habitats were obtained by liquid chromatography-time-of-flight-mass spectrometry(LC-TOF-MS). Principal component analysis

partial least squares-discriminant analysis and cluster analysis were used to compare the differences in chemical profiles among Magnoliae Officinalis Cortex from different varieties and habitats

and adopted to screen out the characteristic chemical constituents that resulted in these differences and to perform mass spectrometry analysis and comparison.

Result:

Eleven characteristic peaks were identified by LC-TOF-MS chromatographic data and reported in the literature.The use of chemical profile could distinguish different habitats of Magnoliae Officinalis Cortex

but could not completely distinguish different varieties of this herb.

Conclusion:

LC-TOF-MS can easily and quickly study on the profile differences of chemical substances in Magnoliae Officinalis Cortex from different varieties and different habitats

the results of this study can provide a theoretical basis for the quality evaluation and pharmacodynamic material basis of this herb.

关键词

Keywords

references

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