HPLC Fingerprint Analysis and Chemistry Pattern Recognition of Wild and Cultivated Notholirion bulbuliferum from Different Regions

Bing-yue YANG; Ben-xiang HU; Lin ZHANG; Gang ZHANG; Yong-gang YAN; Yi-min LI; Jing GAO; Wei WANG; Mei WANG; Liang PENG

doi:10.13422/j.cnki.syfjx.20190215

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HPLC Fingerprint Analysis and Chemistry Pattern Recognition of Wild and Cultivated Notholirion bulbuliferum from Different Regions

Resource and Quality Evaluation | 更新时间：2021-02-09

- HPLC Fingerprint Analysis and Chemistry Pattern Recognition of Wild and Cultivated Notholirion bulbuliferum from Different Regions
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 5, Pages: 187-191(2019)
- 作者机构：
  
  陕西中医药大学　药学院，西安　 712046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190215
  CLC： R284.1;R289;R22
- Received：27 April 2018，
  
  Published Online：02 November 2018，
  
  Published：05 March 2019
- 稿件说明：
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Bing-yue YANG, Ben-xiang HU, Lin ZHANG, et al. HPLC Fingerprint Analysis and Chemistry Pattern Recognition of Wild and Cultivated Notholirion bulbuliferum from Different Regions[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 187-191.
DOI：

Bing-yue YANG, Ben-xiang HU, Lin ZHANG, et al. HPLC Fingerprint Analysis and Chemistry Pattern Recognition of Wild and Cultivated Notholirion bulbuliferum from Different Regions[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(5): 187-191. DOI： 10.13422/j.cnki.syfjx.20190215.

摘要

目的：

建立野生和栽培太白米的HPLC指纹图谱，结合化学模式识别方法对二者进行判别，以期为不同来源太白米药材的质量评价和驯化栽培提供依据。

方法：

利用高效液相色谱法，测定10批野生品和10批栽培品共计20批太白米药材的指纹图谱，建立指纹图谱共有模式，采用中药色谱指纹图谱相似度评价系统（2012A版）计算相似度，运用主成分分析(PCA)，聚类分析(HCA)和最小偏二乘法-判别分析(PLS-DA)对野生与栽培品太白米进行模式识别研究。

结果：

建立了野生与栽培太白米的HPLC指纹图谱共有模式，标定了26个共有峰，20批太白米样品的指纹图谱与对照指纹图谱相似度>0.9。主成分分析、聚类分析和最小偏二乘法-判别分析能对野生与栽培太白米进行明确的区分，表明导致差异的主要化学标记物为对香豆酸等8个成分。

结论：

该文建立的太白米HPLC指纹图谱具有较强的特征性和重复性，与模式识别方法结合起来可有效的评价太白米质量以及区分其野生与栽培品，为太白米的质量控制和驯化栽培提供参考依据。

Abstract

Objective:

To establish the HPLC fingerprint of wild and cultivated

Notholirion bulbuliferum

and recognize them according to the chemical pattern

in the expectation of providing the basis for the quality control and domestication cultivation of

bulbuliferum

of origins.

Method:

Twenty samples of wild and cultivated

bulbuliferum

collected from different origins were detected by HPLC

and a common mode of fingerprint was established. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012A edition) was used to evaluate the similarity of the samples. The differences among the samples were identified by chemical pattern recognition methods

including principal component analysis (PCA)

cluster analysis (HCA) and partial least squares discriminate analysis (PLS-DA).

Result:

The HPLC fingerprint of

bulbuliferum

was obtained

and 26 common peaks were found in the chromatograph. Similarities of all samples were over 0.9

PCA

and HCA and PLS-DA results demonstrated obvious distinctions between wild and cultivated

bulbuliferum

. Eight constituents

such as pcoumaric acid were identified as biomarkers

representing major differences between the two varieties.

Conclusion:

The HPLC chromatogram of

bulbuliferum

developed in this paper has strong characteristics and repeatability. After being combined with the pattern recognition mode

it can be used as an effective method for evaluating the quality of

bulbuliferum

and distinguishing wild and cultivated

bulbuliferum

and provide a reference for the quality control and domestication introduction of

bulbuliferum

关键词

Keywords

references

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