Chemical Constituents of Lipid-soluble Parts of Tinospora sinensis

Xiao-fang ZHU; Ya-juan MA; Wen-ting BAI; Li-ping HUANG; Yu-hui PING; Yi-hui XIE

doi:10.13422/j.cnki.syfjx.20190216

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Chemical Constituents of Lipid-soluble Parts of Tinospora sinensis

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- Chemical Constituents of Lipid-soluble Parts of Tinospora sinensis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 2, Pages: 163-168(2019)
- 作者机构：
  
  江西中医药大学　药学院，南昌　 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190216
  CLC： R284.2; R289; R22
- Received：16 March 2018，
  
  Published Online：26 October 2018，
  
  Published：20 January 2019
- 稿件说明：
移动端阅览
Xiao-fang ZHU, Ya-juan MA, Wen-ting BAI, et al. Chemical Constituents of Lipid-soluble Parts of Tinospora sinensis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 163-168.
DOI：

Xiao-fang ZHU, Ya-juan MA, Wen-ting BAI, et al. Chemical Constituents of Lipid-soluble Parts of Tinospora sinensis[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 163-168. DOI： 10.13422/j.cnki.syfjx.20190216.

摘要

目的：

对藏族药宽筋藤脂溶性部位的化学成分进行分离，并对分离得到的单体化合物进行结构鉴定。

方法：

宽筋藤干燥根茎80 kg，用生药量5倍80%乙醇浸泡1 h，然后沸水回流1 h，滤过，再重复上述步骤，合并2次提取液，回收溶剂得到总浸膏。将所得浸膏用传统溶剂萃取、回收溶液，分别得到石油醚浸膏，二氯甲烷浸膏，乙酸乙酯浸膏，正丁醇浸膏，水浸膏。对其中二氯甲烷萃取部位采用硅胶柱色谱、半制备液相色谱和LH-20型羟丙基葡聚糖凝胶(SephadexLH-20)色谱，重结晶等方法进行分离纯化，综合质谱(ESI-MS)，核磁共振谱(

H-NMR和

C-NMR)等波谱数据并结合文献鉴定化学结构。

结果：

从二氯甲烷萃取部位中分离得到15个化合物，分别鉴定为正十二烷醇(1)，正二十六烷醇(2)，棕榈酸(3)，邻苯二甲酸二丁酯(4)，香草酸(5)，香草醛(6)，夹竹桃素(7)，tinocordifolioside(8)，杜仲树脂酚(9)，异落叶松脂素(10)，橙黄胡椒酰胺(11)，橙黄胡椒乙酰胺(12)，小檗碱(13)，胡萝卜苷(14)，

-谷甾醇(15)。

结论：

其中，化合物除3，4，6，14，15外，其他10个化合物均为首次从该植物中分离得到。

Abstract

Objective:

To isolate the chemical constituents from lipid-soluble parts of Tibetan

Tinospora sinensis

and identify the structure of the monomer compounds.

Method:

Eighty kg of dried rhizomes of

sinensis

were soaked by 80% ethanol (5 times of the dose of crude drug) for 1 hour

boiled under reflux for 1 hour

then and filtered. The above steps were repeated. The two extracts were combined

and the solvent was recovered to obtain a total extract. The obtained extract was extracted with a conventional solvent

and the solution was recovered to obtain petroleum ether extract

methylene chloride extract

ethyl acetate extract

-butanol extract and water extract. Methylene chloride extract was isolated and purified by silicagel column chromatography

semi-preparative liquid chromatography and SephadexLH-20 chromatography; the chemical structure was identified on the basis of ESI-MS

nuclear magnetic resonance (

H-NMR and

C-NMR) spectroscopy data and literatures.

Result:

Fifteen compounds were isolated and identified respectively as

-dodecanol (1)

-hexacosanol (2)

palmitic acid (3)

dibutyl phthalate (4)

vanillic acid (5)

vanillin (6)

apocynin (7)

tinocordifolioside (8)

medioresinol (9)

isolariciresinol (10)

aurantiamide (11)

aurantiamide acetate (12)

berberine (13)

daucosterol (14)

-sitosterol (15).

Conclusion:

Except for 3

the other 10 compounds were isolated from the plant for the first time.

关键词

Keywords

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