Changes of CCl<sub>4</sub>-induced of Liver Injury in Rats for 24 Hours

Ping-sheng ZHU; Yu MENG; Shuang-nan FU; Ming-san MIAO; Yan-jie JIAO; Zheng-wang ZHU; Da GAO

doi:10.13422/j.cnki.syfjx.20190222

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Changes of CCl₄-induced of Liver Injury in Rats for 24 Hours

Topic on Changes of Biomarkers in Animal Models of Early Liver Injury | 更新时间：2021-02-09

- Changes of CCl₄-induced of Liver Injury in Rats for 24 Hours
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 2, Pages: 124-128(2019)
- 作者机构：
  
  河南中医药大学，郑州　 450046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190222
  CLC： R2-0; R22; R285.5
- Received：21 July 2018，
  
  Published Online：26 October 2018，
  
  Published：20 January 2019
- 稿件说明：
移动端阅览
Ping-sheng ZHU, Yu MENG, Shuang-nan FU, et al. Changes of CCl₄-induced of Liver Injury in Rats for 24 Hours[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 124-128.
DOI：

Ping-sheng ZHU, Yu MENG, Shuang-nan FU, et al. Changes of CCl₄-induced of Liver Injury in Rats for 24 Hours[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 124-128. DOI： 10.13422/j.cnki.syfjx.20190222.

摘要

目的：

采用四氯化碳(CCl

)复制大鼠肝损伤的动物模型，研究肝损伤早期生物标志物谷氨酸脱氢酶(GLDH)，嘌呤核苷酸磷酸化酶(PNP)，

-谷胱甘肽-

-转移酶(

-GST)，精氨酸酶1(Arg1)的动态变化，为急性肝损伤的早期发现提供实验依据。

方法：

48只Wistar大鼠，随机分为正常组和模型组，模型组腹腔注射10%的CCl

橄榄油溶液10 mL·kg

－1

，禁食不禁水，于3，6，12，24 h后处死各组动物，测定血清肝功能丙氨酸氨基转移酶(ALT)，天门冬氨酸氨基转移酶(AST)，胆红素(TBIL)，碱性磷酸酶(ALP)的水平，

-GST，Arg1，GLDH，PNP水平，肝匀浆超氧化物歧化酶(SOD)，谷胱甘肽(GSH)，丙二醛(MDA)的水平。

结果：

与正常组比较，给药3 h后ALT，AST，TBIL，

-GST，Arg1，GLDH，PNP，MDA水平显著升高，SOD明显下降(

0.01)，给药6，12 h后ALT，AST，

-GST，Arg1，GLDH，TBIL，ALP，MDA水平显著升高，GSH，SOD明显下降(

0.05，

0.01)，给药24 h后ALT，AST，

-GST，Arg1，TBIL，ALP，MDA水平显著升高，GSH，SOD显著下降(

0.01)。

结论：

-GST，Arg1，GLDH，PNP较传统肝功能检测指标具有较好的灵敏性，可用于CCl

致大鼠肝损伤早期检测。

Abstract

Objective:

To replicate the animal model of liver injury in rats by using carbon tetrachloride (CCl

)

investigate the dynamic changes of early biomarkers of liver injury

namely glutamate dehydrogenase (GLDH)

purine nucleotide phosphorylase(PNP)

-dynamic changes of glutathione-S-transferase (

-GST) and arginase 1(Arg1)

and provide experimental evidence for early detection of acute liver injury.

Method:

Forty-eight Wistar rats were randomly divided into a blank group and a model group. The model group was intraperitoneally injected with 10 mL·kg

-1

10% CCl

olive oil solution

fasting but except water. Animals were sacrificed at 3

and 24 h. The serum liver function alanine aminotransferase(ALT)

aspartate aminotransferase (AST)

bilirubin (TBIL)

alkaline phosphatase (ALP) levels

-GST

Arg1

GLDH

PNP levels

and liver homogenate superoxide dismutase (SOD)

glutathione (GSH)

malondialdehyde (MDA) levels were then detected.

Result:

As compared with blank group

the levels of ALT

AST

TBIL

-GST

Arg1

GLDH

PNP and MDA were increased significantly 3 h after administration

and SOD was decreased significantly(

0.01). After 6

12 h

the levels of ALT

AST

-GST

ARG-

GLDH

TBIL

ALP and MDA were increased significantly

while GSH and SOD were decreased significantly (

0.05

0.01). After 24 h

the levels of ALT

AST

-GST

Arg1

TBIL

ALP and MDA were significantly increased

while GSH and SOD were significantly decreased (

0.01).

Conclusion:

-GST

Arg1

GLDH and PNP have better sensitivity than traditional liver function test indicators

and can be used for early detection of liver injury induced by CCl

in rats.

关键词

Keywords

references

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Related Author

Ping-sheng ZHU

Yan-jie JIAO

Shuang-nan FU

Ming-san MIAO

Yu MENG

Da GAO

Zheng-wang ZHU

ZHAO Jinghan

Related Institution

Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-yao

School of Pharmacy， Henan University of Chinese Medicine

The First Clinical Medical College of Henan University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Ningxia Medical University

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⁰

Changes of CCl4-induced of Liver Injury in Rats for 24 Hours

DOI：10.13422/j.cnki.syfjx.20190222

摘要

Abstract

关键词

Keywords

references

Changes of CCl₄-induced of Liver Injury in Rats for 24 Hours