Effect of Bushen Tongluo Prescription on TGF-<italic style="font-style: italic">β</italic><sub>1</sub>/Smads Pathway in Pulmonary Fibrosis Rats

Xiao LIU; Wen-tao YU; He-chao JIN; Jia-xing JING; Feng-zhe SU

doi:10.13422/j.cnki.syfjx.20190229

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Effect of Bushen Tongluo Prescription on TGF-β₁/Smads Pathway in Pulmonary Fibrosis Rats

Pharmacology | 更新时间：2021-02-09

- Effect of Bushen Tongluo Prescription on TGF-β₁/Smads Pathway in Pulmonary Fibrosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 2, Pages: 96-102(2019)
- 作者机构：
  
  1.河北中医学院　基础医学院，石家庄　 050200；
  2.河北省心脑血管病中医药防治重点实验室，石家庄　 050091；
  3.北京市通州区中西医结合医院，北京　 101100
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190229
  CLC： R289; R289.5; R256.1
- Received：16 August 2018，
  
  Published Online：26 October 2018，
  
  Published：20 January 2019
- 稿件说明：
移动端阅览
Xiao LIU, Wen-tao YU, He-chao JIN, et al. Effect of Bushen Tongluo Prescription on TGF-β₁/Smads Pathway in Pulmonary Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 96-102.
DOI：

Xiao LIU, Wen-tao YU, He-chao JIN, et al. Effect of Bushen Tongluo Prescription on TGF-β₁/Smads Pathway in Pulmonary Fibrosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(2): 96-102. DOI： 10.13422/j.cnki.syfjx.20190229.

摘要

目的：

观察补肾通络方对博莱霉素所致肺纤维化大鼠肺组织病理及转化生长因子

(TGF-

)/Smads通路蛋白的影响，揭示该方抑制肺纤维化的作用机制。

方法：

48只清洁级雄性SD大鼠随机分为正常组8只，模型组、补肾通络低、高剂量组、氢化可的松琥珀酸钠组各10只。除正常组外，各组均采用博莱霉素法诱导肺纤维化制备模型。从术后第3天开始，补肾通络高、低剂量组分别给予14.2，7.1 g·kg

－1

补肾通络方药灌胃，氢化可的松琥珀酸钠组给予4.58 g·kg

－1

氢化可的松琥珀酸钠溶液腹腔注射，正常组和模型组给予等体积生理盐水灌胃，共治疗28 d。大鼠处死后测定血清羟脯氨酸(HYP)，透明质酸(HA)，层粘连蛋白(LN)的含量，取肺组织，采用苏木精-伊红染色法(HE)及马松(Masson)三色染色法观察肺组织病理形态变化，采用实时荧光定量PCR法(Real-time PCR)和蛋白免疫印迹法(Western blot)观察各组大鼠肺组织TGF-

，Smad2，Smad3，Smad7等mRNA和蛋白表达变化。

结果：

与正常组相比，模型组大鼠肺纤维化明显，HYP，HA，LN含量升高(

0.05，

0.01)，肺组织TGF-

，Smad2，Smad3蛋白和mRNA表达升高(

0.05，

0.01)，Smad7蛋白和mRNA表达降低(

0.01)；与模型组相比，补肾通络高剂量组血清HYP，HA，LN的含量下降(

0.05，

0.01)肺组织TGF-

，Smad2，Smad3蛋白和mRNA表达降低(

0.05)，Smad7蛋白和mRNA表达升高(

0.01)。

结论：

补肾通络方可有效改善肺纤维化的病变进程，其机制可能与调节TGF-

/Smads通路有关。

Abstract

Objective:

To observe the effect of Bushen Tongluo prescription on the pathogenesis of lung fibrosis induced by bleomycin and the transforming growth factor

(TGF-

)/Smads pathway protein in rats

and reveal the mechanism of this formula in inhibiting pulmonary fibrosis.

Method:

Totally 48 male SD rats were randomly divided into five groups: normal group(8 rats)

model group(10 rats)

high and low-dose Bushen Tongluo group(10 rats)and hydrocortisone sodium succinate group(10 rats). The model of pulmonary fibrosis was made with bleomycin except for normal group.Since day 3 after surgery

14.2

7.1 g·kg

-1

Bushen Tongluo prescription were given by

in high and low-dose Bushen Tongluo groups

and 4.58 g·kg

-1

hydrocortisone sodium succinate was given by gavage(

) in normal group and model group.All of the rats were put to death after 28 days.The contents of hydroxyproline (HYP)

hyaluronic acid (HA) and laminin (LN) were measured after the death of the rats.Hematoxylin-eosin staining (HE) and Masson tricolor staining were used to observe the pathological changes of lung tissue. Real-time PCR and Western blot were used to observe the changes of mRNA and protein expressions of lung tissues TGF-

Smad2

Smad3

Smad7 in each group.

Result:

Compared with normal group

the level of pulmonary fibrosis was more significant in model group

and the serum HYP

HA and LN were increased obviously(

0.05

0.01). The expressions of TGF-

Smad2

Smad3 proteins and mRNA in lung tissues were increased obviously(

0.05

0.01). The expressions of Smad7 protein and mRNA were decreased significantly(

0.01). Compared with model group

the contents of HYP

HA and LN in high-dose Bushen Tongluo group were decreased obviously(

0.05

0.01)

the expressions of lung tissues TGF-

Smad2

Smad3 protein and mRNA were decreased obviously(

0.05)

and the expressions of Smad7 protein and mRNA was increased significantly(

0.01).

Conclusion:

Bushen Tongluo prescription can effectively improve the pathological process of pulmonary fibrosis

and the mechanism may be related to the regulation of TGF-

/Smads pathway.

关键词

Keywords

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⁰

Effect of Bushen Tongluo Prescription on TGF-β1/Smads Pathway in Pulmonary Fibrosis Rats

DOI：10.13422/j.cnki.syfjx.20190229

摘要

Abstract

关键词

Keywords

references

Effect of Bushen Tongluo Prescription on TGF-β₁/Smads Pathway in Pulmonary Fibrosis Rats