Effect of Compatibility of Alcohol Extract of Magnoliae Officinalis Cortex and Alcohol Extract of Polygalae Radix on Fecal Metabolites of Rats

Rong MA; Qian XIE; Jian WANG; Li-hua HUANG; Xiao MA; Nian CHEN; Tai-wei DONG; Xiao-qing GUO; Ya-mei FAN

doi:10.13422/j.cnki.syfjx.20190303

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Effect of Compatibility of Alcohol Extract of Magnoliae Officinalis Cortex and Alcohol Extract of Polygalae Radix on Fecal Metabolites of Rats

Compatibility | 更新时间：2021-02-09

- Effect of Compatibility of Alcohol Extract of Magnoliae Officinalis Cortex and Alcohol Extract of Polygalae Radix on Fecal Metabolites of Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 11, Pages: 1-7(2019)
- 作者机构：
  
  成都中医药大学　药学院，中药材标准化教育部重点实验室，四川省中药资源系统研究与开发利用重点实验室——省部共建国家重点实验室培育基地，成都　 611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190303
  CLC： R22; R24; R28; C37; R969.1
- Received：13 September 2018，
  
  Published Online：19 October 2018，
  
  Published：05 June 2019
- 稿件说明：
移动端阅览
Rong MA, Qian XIE, Jian WANG, et al. Effect of Compatibility of Alcohol Extract of Magnoliae Officinalis Cortex and Alcohol Extract of Polygalae Radix on Fecal Metabolites of Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 1-7.
DOI：

Rong MA, Qian XIE, Jian WANG, et al. Effect of Compatibility of Alcohol Extract of Magnoliae Officinalis Cortex and Alcohol Extract of Polygalae Radix on Fecal Metabolites of Rats[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(11): 1-7. DOI： 10.13422/j.cnki.syfjx.20190303.

摘要

目的：

考察厚朴醇提物、远志醇提物及二者配伍对大鼠粪便代谢物的影响，分析其潜在的代谢通路，为探索厚朴缓解远志所致胃肠动力障碍的机制提供实验依据。

方法：

将40只SD雄性大鼠随机分为空白组，厚朴醇提物组(3.50 g·kg

－1

)，远志醇提物组(1.75 g·kg

－1

)，配伍组(3.50 g·kg

－1

＋1.75 g·kg

－1

)，连续灌胃给药3 d，收集大鼠末次给药24 h内的粪便，采用UPLC-Q-TOF-MS采集粪便代谢物数据，流动相乙腈(A)-0.1%甲酸水溶液(B)梯度洗脱(0～0.5 min，3%A；0.5～12.5 min，3%～70%A；12.5～13 min，70%～90%A；13～16 min，90%～97%A；16～17 min，97%～100%A；17～22 min，100%A；22～23 min，100%～3%A)，电喷雾离子源，正、负离子模式数据采集范围

50～1 200；借助Progenesis QI v2.0，SIMCA-P 14.0，SPSS 20.0，MetaboAnalyst 4.0等软件进行多元分析，筛选特征代谢标志物并分析其相关代谢通路。

结果：

共筛选出5-亚胺甲基四氢叶酸，

-3-羟基犬尿氨酸，7，8-二氢蝶酸等17个特征代谢标志物，其相关代谢通路为不饱和脂肪酸的生物合成、亚油酸代谢、维生素B

代谢、叶酸生物合成等。

结论：

厚朴缓解远志所致胃肠动力障碍的作用机制可能与嘌呤代谢、叶酸生物合成、色氨酸代谢、初级胆汁酸生物合成有关。

Abstract

Objective:

To investigate the effect of alcohol extract of Magnoliae Officinalis Cortex

alcohol extract of Polygalae Radix and their compatibility on fecal metabolites of rats

analyze its potential metabolic pathways

and provide experimental basis for exploring the possible mechanism of Magnoliae Officinalis Cortex relieving gastrointestinal motility disorders induced by Polygalae Radix.

Method:

Forty male SD rats were randomly divided into the normal group

alcohol extract of Magnoliae Officinalis Cortex group(3.50 g·kg

-1

)

alcohol extract of Polygalae Radix group(1.75 g·kg

-1

) and compatibility group (3.5 g·kg

-1

of alcohol extract of Magnoliae Officinalis Cortex+ 1.75 g·kg

-1

of alcohol extract of Polygalae Radix). Fecal samples were collected within 24 h after continuous gavage for 3 days.The fecal metabolites in each group was detected by ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS)

mobile phase was acetonitrile-0.1%formic acid solution for gradient elution

data collection range was

50-1 200 under positive and negative ion mode of electrospray ionization.The characteristic biomarkers and corresponding metabolic pathways were analyzed or screened by Progenesis QI v2.0

SIMCA-P 14.0

SPSS 20.0

MetaboAnalyst 4.0 and other softwares.

Result:

A total of 17 characteristic metabolic markers were screened out

including 5-formiminotetrahydrofolic acid

-3-hydroxykynurenine

8-dihydropteroic acid

etc.The main related pathways included biosynthesis of unsaturated fatty acids

linoleic acid metabolism

vitamin B

metabolism

etc.

Conclusion:

The mechanism of Magnoliae Officinalis Cortex relieving gastrointestinal motility disorders induced by Polygalae Radix may be related to purine metabolism

folate biosynthesis

tryptophan metabolism and primary bile acid biosynthesis.

关键词

Keywords

references

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