Effect of Rhei Radix et Rhizoma on Gastrointestinal Function of Normal Rats Before and After Simmering

Zhi ZHANG; Ting-xian LI; Liu XU; Jing XIE; De-xuan KONG; Guang-zhong WANG

doi:10.13422/j.cnki.syfjx.20190314

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Effect of Rhei Radix et Rhizoma on Gastrointestinal Function of Normal Rats Before and After Simmering

Pharmacy and Processing | 更新时间：2021-02-09

- Effect of Rhei Radix et Rhizoma on Gastrointestinal Function of Normal Rats Before and After Simmering
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 12, Pages: 140-144(2019)
- 作者机构：
  
  湖北中医药大学　药学院，武汉　 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20190314
  CLC： R22; R943.1; R28; C37; TS193
- Received：04 September 2018，
  
  Published Online：19 November 2018，
  
  Published：20 June 2019
- 稿件说明：
移动端阅览
Zhi ZHANG, Ting-xian LI, Liu XU, et al. Effect of Rhei Radix et Rhizoma on Gastrointestinal Function of Normal Rats Before and After Simmering[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 140-144.
DOI：

Zhi ZHANG, Ting-xian LI, Liu XU, et al. Effect of Rhei Radix et Rhizoma on Gastrointestinal Function of Normal Rats Before and After Simmering[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(12): 140-144. DOI： 10.13422/j.cnki.syfjx.20190314.

摘要

目的：

探讨大黄对正常大鼠胃肠功能的影响及其作用机制，并比较其煨制前后作用差异。

方法：

将健康SD大鼠随机分为空白组、生大黄高、中、低剂量组(1.5，3.0，6.0 g·kg

－1

·d

－1

)以及煨大黄高、中、低剂量组(给药剂量同生品)共7组，每组8只，连续灌胃给药10 d，通过大鼠的体质量、进食量、胃内容物残留率、小肠推进率、胃系数以及胃组织形态观察评价大黄对胃肠功能的影响；采用酶联免疫吸附测定法(ELISA)检测大鼠血浆胃动素(MTL)，血清胃泌素(GAS)，肿瘤坏死因子(TNF)-

，白细胞介素-10(IL-10)的含量，探究大黄对胃肠功能产生影响的作用机制，比较大黄煨制前后对正常大鼠胃肠功能的影响。

结果：

各给药组不同程度的减缓了大鼠体质量增长，抑制胃排空、小肠推进，引发炎症反应，增加胃黏膜损伤。与空白组相比，除煨大黄低、中剂量组外，其他各组大鼠胃内残留率、胃系数和TNF-

含量显著升高(

0.05，

0.01)；IL-10含量显著降低(

0.01)；除煨大黄低剂量组外，其他各组大鼠体质量，小肠推进率，GAS和MTL含量显著降低(

0.05，

0.01)。与等剂量生大黄组相比，煨大黄各剂量组大鼠体质量，小肠推进率，GAS，IL-10，MTL含量均有不同程度的升高；胃系数、胃内容物残留率和TNF-

含量均有不同程度的降低。

结论：

大黄导致胃肠功能紊乱的原因可能与其抑制胃肠激素和促进炎症细胞因子的表达有关；大黄煨制后，大黄“苦寒败胃”的作用减弱，为大黄的临床合理用药提供了实验依据。

Abstract

Objective:

To investigate the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats and explore its therapeutic mechanism

and compare the difference of this herb before and after simmering.

Method:

Healthy SD rats were randomly divided into 7 groups

including the blank group

the high

medium and low dose groups of raw Rhei Radix et Rhizoma(1.5

3.0

6.0 g·kg

-1

·d

-1

) and the high

medium and low dose groups of simmered Rhei Radix et Rhizoma(1.5

3.0

6.0 g·kg

-1

·d

-1

)

eight rats in each group were continuous intragastric administration for 10 days

the effect of Rhei Radix et Rhizoma on gastrointestinal function of rats were evaluated by body weight

food intake

gastric remnant rate

small intestine propelling rate

stomach weight coefficient and gastrointestinal tissue morphology; enzyme-linked immunosorbent assay(ELISA) was used to detect contents of motilin(MTL) in rat plasma and gastrin(GAS)

tumor necrosis factor-

(TNF-

)

interleukin-10(IL-10) in rat serum

the mechanism of effect of Rhei Radix et Rhizoma on gastrointestinal function was explored and the effect of Rhei Radix et Rhizoma on gastrointestinal function of normal rats before and after simmering was compared.

Result:

In each administration group

weight growth of rats was slowed down

gastric emptying and intestinal propulsion were inhibited

inflammatory reaction was triggered

and gastric mucosal injury was increased.Compared with the blank group

except for the low-dose and medium-dose groups of simmered Rhei Radix et Rhizoma

stomach weight coefficient

gastric remnant rate and content of TNF-

of rats were significantly increased(

0.05

0.01)

and the content of IL-10 was significantly reduced

in addition to the low-dose group of simmered Rhei Radix et Rhizoma

the weight

small intestine propelling rate

contents of GAS and MTL of rats were significantly decreased(

0.05

0.01). Compared with the isodose raw Rhei Radix et Rhizoma group

the weight

small intestine propelling rate

contents of GAS

IL-10 and MTL were all increased with different degrees

the gastric weight coefficient

gastric remnant rate and content of TNF-

of rats were all decreased to different degrees.

Conclusion:

Gastrointestinal dysfunction caused by Rhei Radix et Rhizoma is related to inhibiting the expression of gastrointestinal hormones and promoting the expression of inflammatory cytokines

in addition

the effect of " stomach injured by bitterness and cold property" of Rhei Radix et Rhizoma is weakened after simmering

which provides scientific basis for clinical rational use of Rhei Radix et Rhizoma.

关键词

Keywords

references

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